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16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting BJM
Navarro-Noya,Yendi; Hernández-Rodríguez,César; Zenteno,Juan C; Buentello-Volante,Beatriz; Cancino-Díaz,Mario E; Jan-Roblero,Janet; Cancino-Díaz,Juan C.
Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophthalmitis; Staphylococcus epidermidis; PCR; DGGE.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100033
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Stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl ether Electron. J. Biotechnol.
Alfonso-Gordillo,Guadalupe; Cristiani-Urbina,Eliseo; Flores-Ortiz,César Mateo; Peralta,Humberto; Cancino-Díaz,Juan Carlos; Cruz-Maya,Juan Antonio; Jan-Roblero,Janet.
Background: Methyl tert-butyl ether (MTBE) is a pollutant that causes deleterious effects on human and environmental health. Certain microbial cultures have shown the ability to degrade MTBE, suggesting that a novel bacterial species capable of degrading MTBE could be recovered. The goal of this study was to isolate, identify and characterize the members of a bacterial consortium capable of degrading MTBE. Results: The IPN-120526 bacterial consortium was obtained through batch enrichment using MTBE as the sole carbon and energy source. The cultivable fraction of the consortium was identified; of the isolates, only Stenotrophomonas maltophilia IPN-TD and Sphingopyxis sp. IPN-TE were capable of degrading MTBE. To the best of our knowledge, this report is the...
Tipo: Journal article Palavras-chave: Bacterial consortium; Biodegradation; MTBE; Sphingopyxis sp; Stenotrophomonas maltophilia.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500003
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