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Chilean IPNV isolates: Robustness analysis of PCR detection Electron. J. Biotechnol.
Jorquera,Esteban; Morales,Paz; Tapia,David; Torres,Pamela; Eissler,Yoanna; Espinoza,Juan C; Conejeros,Pablo; Kuznar,Juan.
Background: The genomes of several infectious pancreatic necrosis viruses (IPNVs) isolated in Chile were sequenced with a single amplification approach for both segments A and B. The resulting sequences were then used to determine the conservation of the primer-binding regions used in polymerase chain reaction (PCR)-based diagnostic methods proposed in the literature. Thus, the robustness of each technique was studied, particularly the eventual effect of further mutations within the primer-binding sites. Results: On analysis, most methods currently used to detect Chilean IPNV varieties were deemed adequate. However, the primers were designed to be genogroup specific, implying that most detection methods pose some risk of detecting all strains prevalent in...
Tipo: Journal article Palavras-chave: IPNV detection; Mismatch's Tm analysis; QPCR.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000200005
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