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| Ueno, S.; Le Provost, G.; Léger, V.; Klopp, C.; Noirot, C.; Frigerio, J.M.; Salin, F.; Salse, J.; Abrouk, M.; Murat, F.; Brendel, O.; Derory, J.; Abadie, P.; Léger, P.; Cabane, C.; Barré, A.; De Daruvar, A.; Couloux, A.; Wincker, P.; Reviron, M.-P.; Kremer, A.; Plomion, C.. |
| Background: The Fagaceae family comprises about 1,000 woody species worldwide. About half belong to the Quercus family. These oaks are often a source of raw material for biomass wood and fiber. Pedunculate and sessile oaks, are among the most important deciduous forest tree species in Europe. Despite their ecological and economical importance, very few genomic resources have yet been generated for these species. Here, we describe the development of an EST catalogue that will support ecosystem genomics studies, where geneticists, ecophysiologists, molecular biologists and ecologists join their efforts for understanding, monitoring and predicting functional genetic diversity.Results: We generated 145,827 sequence reads from 20 cDNA libraries using the Sanger... |
| Tipo: Journal Article |
Palavras-chave: POLYMORPHISME NUCLÉOTIDIQUE SIMPLE; RESSOURCE GÉNOMIQUE; EXTRACTION D'ARN; CLONAGE D'ADN; OUTIL D'ANALYSE. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011fe822ff&uri=/notices/prodinra1/2011/03/ |
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| Lapegue, Sylvie; Harrang, Estelle; Heurtebise, Serge; Flahauw, Emilie; Donnadieu, C.; Gayral, P.; Ballenghien, M.; Genestout, L.; Barbotte, L.; Mahla, R.; Haffray, P.; Klopp, C.. |
| Use of SNPs has been favored due to their abundance in plant and animal genomes, accompanied by the falling cost and rising throughput capacity for detection and genotyping. Here, we present in vitro (obtained from targeted sequencing) and in silico discovery of SNPs, and the design of medium-throughput genotyping arrays for two oyster species, the Pacific oyster, Crassostrea gigas, and European flat oyster, Ostrea edulis. Two sets of 384 SNP markers were designed for two Illumina GoldenGate arrays and genotyped on more than 1000 samples for each species. In each case, oyster samples were obtained from wild and selected populations and from three-generation families segregating for traits of interest in aquaculture. The rate of successfully genotyped... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; GoldenGate technology; Ostrea edulis; Oysters; SNP genotyping. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00172/28369/26677.pdf |
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| Fleury, E.; Huvet, A.; Lelong, C.; de Lorgeril, J.; Boulo, V.; Gueguen, Y.; Bachère, E.; Tanguy, A.; Moraga, D.; Fabioux, C.; Lindeque, P.; Shaw, J.; Reinhardt, R.; Prunet, P.; Davey, G.; Lapègue, S.; Sauvage, C.; Corporeau, C.; Moal, J.; Gavory, F.; Wincker, P.; Moreews, F.; Klopp, C.; Mathieu, M.; Boudry, P.; Favrel, P.. |
| Tipo: Journal Article |
Palavras-chave: COQUILLAGE; HUITRE; CRASSOSTREA GIGAS; EXPRESSION DES GENES; BASE DE DONNEES; GIGASDATABASE. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009dfd43c72&uri=/notices/prodinra1/2009/11/ |
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| Désert, C.; Duclos, M.; Blavy, P.; Lecerf, F.; Moreews, F.; Klopp, C.; Aubry, M.; Herault, F.; Le Roy, P.; Berri, C.; Douaire, M.; Diot, C.; Lagarrigue, S.. |
| Tipo: Journal Article |
Palavras-chave: FOIE. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20098325a42&uri=/notices/prodinra1/2009/07/ |
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