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Identification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivars. Repositório Alice
MOFATTO, L. S.; CARNEIRO, F. de A.; VIEIRA, N. G.; DUARTE, K. E.; VIDAL, R. O.; ALEKCEVETCH, J. C.; COTTA, M. G.; VERDEIL, J-L.; LAPEYRE-MONTES, F.; LARTAUD, M.; LEROY, T.; DE BELLIS, F.; POT, D; RODRIGUES, G. C.; ALSARELLA CARAZZOLLE, M.; PEREIRA, G. A. G.; ANDRADE, A. C.; MARRACCINI, P..
BACKGROUND: Drought is a widespread limiting factor in coffee plants. It affects plant development, fruit production, bean development and consequently beverage quality. Genetic diversity for drought tolerance exists within the coffee genus. However, the molecular mechanisms underlying the adaptation of coffee plants to drought are largely unknown. In this study, we compared the molecular responses to drought in two commercial cultivars (IAPAR59, drought-tolerant and Rubi, drought-susceptible) of Coffea arabica grown in the field under control (irrigation) and drought conditions using the pyrosequencing of RNA extracted from shoot apices and analysing the expression of 38 candidate genes. RESULTS: Pyrosequencing from shoot apices generated a total of 34.7...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Candidate gene; Coffee; Differential gene expression; RNA-Seq; Real-time PCR (RT-qPCR); Drought; Coffea arabica.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1043710
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Reactive oxygen species and cellular interactions between Mycosphaerella fijiensis and banana. Repositório Alice
CAVALCANTE, M. de J. B.; ESCOUTE, J.; MADEIRA, J. P.; ROMERO, R. E.; NICOLE, M. R.; OLIVEIRA, L. C. de; HAMELIN, C.; LARTAUD, M.; VERDEIL, J. L..
Globally, the banana plant (Musa spp) is the fourth most important crop after rice, wheat and corn (based on production in tons). It is cultivated in more than 100 tropical and subtropical countries, mainly by small producers and is a fundamental food source for millions of people. Black leaf streak disease (BLSD), caused by Mycosphaerella fijiensis Morelet (sexual phase) or Paracercospora fijiensis (Morelet) Deighton (asexual phase), is the main disease affecting the world?s banana culture. This disease has a wide geographical distribution accounting for losses exceeding 50% of global banana production. We conducted a comparative histocytological study on the kinetics of the infection process using three banana genotypes with phenotypes that differ in...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Doença de planta; Banana; Musa sp; Fungo; Sigatoka negra; Mycosphaerella fijiensis; Interação genética; Musa acuminata; Variedade resistente; Análise comparativa; Peroxidase; Plant diseases and disorders; Bananas; Fungi; Black sigatoka; Genetic techniques and protocols; Enfermedades y desórdenes de las plantas; Bananos; Peroxidasa; Técnicas y protocolos genéticos.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/873336
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