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Registros recuperados: 9
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A multibiomarker approach on the Atlantic tomcod (Microgadus tomcod) in the St. Lawrence Estuary ArchiMer
Dupuy, Celie; Couillard, Catherine M.; Laroche, Jean; Nellis, Pierre; Brousseau, Pauline; Fournier, Michel.
A multibiomarker approach was developed on juvenile Atlantic tomcod (Microgadus tomcod) to evaluate the pertinence of this approach for low-cost screening assessment of the environmental quality of various coastal sites within estuaries. Several biometric indices and biomarkers (ethoxyresorufin-O-deethylase (EROD) activity, metallothionein concentration, and immune responses) were investigated on immature and maturing tomcods (a parts per thousand currency sign31 months) collected in four environmentally contrasted sites in the St. Lawrence Estuary (SLE). Simultaneous examination of various age classes provides the opportunity to detect short-term responses in sensitive young-of-the-year fish (e.g., EROD induction) and longer-time effects associated with...
Tipo: Text Palavras-chave: Biometric indexes; EROD; Metallothionein; Immunotoxicity; Pollution; Fish.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00125/23578/21501.pdf
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Biologie et pêche traditionnelle des crevettes pénéides dans l'embouchure d'un fleuve en milieur semi-aride (sud-ouest de Madagascar) ArchiMer
Laroche, Jean; Benoely,; Siaviri, A.
Traditional fishing using beach seines was studied over a period of one year at the mouth of the Onilahy river. The catches/efforts were recorded on a daily basis, biological sampling and the measurement of hydrological parameters being carried out twice a month. The moderate variations of salinity and temperature (14 < salinity < 35; 25 degrees C < temperature < 30 degrees C) which were observed in the estuary can be explained by the semiarid climate of the area. Four species of penaeid shrimps are to be found on the Onilahy: Metapenaeus monoceros, Panaeus indicus, Penaeus monodon and Penaeus japonicus, which were mainly caught at the subadult/adult stage with a Carapace length between 10 and 50 mm. The presence of these important sizes at the...
Tipo: Text Palavras-chave: Pêche; Estuaire; Dynamique population; Croissance; Taille corporelle; Cycle évolutif; Zone semi aride; Madagascar; Penaeus japonicus; Penaeus monodon; Penaeus indicus; Metapenaeus monoceros; Rivière Onilahy; Afrique; Milieu saumâtre; Macrura; Decapoda; Crustacea; Arthropoda; Invertebrata; Fishing; Estuaries; Population dynamics; Growth; Body size; Life history; Semi arid zone; Malagasy Republic; Penaeus japonicus; Penaeus monodon; Penaeus indicus; Africa; Brackish water environment; Macrura; Decapoda; Crustacea; Arthropoda; Invertebrata.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00097/20828/42822.pdf
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Effects of bioactive extracellular compounds and paralytic shellfish toxins produced by Alexandrium minutum on growth and behaviour of juvenile great scallops Pecten maximus ArchiMer
Borcier, Elodie; Morvezen, Romain; Boudry, Pierre; Miner, Philippe; Charrier, Gregory; Laroche, Jean; Hegaret, Helene.
Dinoflagellates of the genus Alexandrium are a major cause of harmful algal blooms (HABs) that have increasingly disrupted coastal ecosystems for the last several decades. Microalgae from the genus Alexandrium are known to produce paralytic shellfish toxins (PST) but also bioactive extracellular compounds (BEC) that can display cytotoxic, allelopathic, ichtyotoxic or haemolytic effects upon marine organisms. The objective of this experimental study was to assess the effects of PST and BEC produced by A. minutum upon juvenile great scallops Pecten maximus. Scallops were exposed for one week to two different strains of A. minutum, the first producing both PST and BEC and the second producing only BEC. Escape response to starfish, daily shell growth,...
Tipo: Text Palavras-chave: Harmful Algal Bloom (HAB); Alexandrium minutum; Pecten maximus; Paralytic Shellfish Toxins (PST); Extracellular compounds; Behaviour; Histology.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00368/47878/47893.pdf
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Genetic structure of a commercially exploited bivalve, the great scallop Pecten maximus, along the European coasts ArchiMer
Morvezen, Romain; Charrier, Gregory; Boudry, Pierre; Chauvaud, Laurent; Breton, Florian; Strand, Oivind; Laroche, Jean.
The great scallop Pecten maximus is harvested in several European countries and fisheries targeting this species are severely regulated by fishing quotas. In addition, hatchery-based population enhancement has been developed in some countries to provide alternative or complementary production. The genetic structure of wild populations of P. maximus and the potential impact of aquaculture on the genetic diversity of this species remains poorly documented. In this study, we explored the genetic structure of P. maximus using 12 microsatellite markers, considering 14 populations sampled from Galicia (Spain) to the North of Norway, and one population of Pecten jacobaeus (L., 1758) from the Lion Gulf (Mediterranean Sea). Results indicated a clear differentiation...
Tipo: Text Palavras-chave: Great scallop; Pecten maximus; Microsatellites; Population genetics; Aquaculture.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00311/42251/41692.pdf
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GenoPopTaille. Moving stock assessment into the future ArchiMer
Marandel, Florianne; Biais, Gerard; Bidault, Adeline; Charrier, Grégory; Cornette, Florence; Lapegue, Sylvie; Laroche, Jean; Le Cam, Sabrina; Stephan, Eric; Trenkel, Verena; Lorance, Pascal.
At the ecosystem level, sustainable exploitation of fisheries resources depends not only on the status of target species but also on that of bycatch species, some of which are even more sensitive to exploitation. This is the case for a number of elasmobranchs (skates, rays and sharks) species whose abundance declined during the 20th century. Further, the biology of elamobranchs is still poorly known and traditional fisheries stock assessment methods using fisheries catches and scientific survey data for estimating abundance are expensive or even inapplicable due to the small numbers observed. The GenoPopTaille project attempts to apply to the case of the thornback ray (Raja clavata) recent genetic-based methods for absolute population abundance estimation...
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Ano: 2016 URL: http://archimer.ifremer.fr/doc/00343/45439/44960.pdf
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Histopathological lesions and DNA adducts in the liver of European flounder (Platichthys flesus) collected in the Seine estuary versus two reference estuarine systems on the French Atlantic coast ArchiMer
Cachot, Jerome; Cherel, Yan; Larcher, Thibaut; Pfohl-leszkowicz, Annie; Laroche, Jean; Quiniou, Louis; Morin, Jocelyne; Schmitz, Julien; Burgeot, Thierry; Pottier, Didier.
An epidemiological survey was conducted in the Seine estuary and in two smaller and relatively preserved estuaries on the French Atlantic coast in order to estimate the occurrence of liver lesions in European flounder, Platichthys flesus, and also to seek putative risk factors for the recorded pathologies. Four hundred and seventy-eight fish of both sexes and of different size ranges were sampled in the three studied areas, 338 of which in the Seine estuary. All fish were examined for histopathological liver lesions, while DNA adducts and otoliths were analyzed on a subsample. Five categories of hepatic lesions were recorded with the following prevalence for the Seine estuary: 36.7 % inflammations, 8 % parasites (mainly encysted nematodes), 6.5 % foci of...
Tipo: Text Palavras-chave: Epidemiological study; European flounder; Seine estuary; Liver histopathology; Necrosis; FCA; Tumors; Inflammations; Parasites; DNA adducts; Pollution.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00125/23576/21498.pdf
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Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas ArchiMer
Tanguy, Arnaud; Boutet, Isabelle; Boudry, Pierre; Degremont, Lionel; Laroche, Jean; Moraga, Dario.
Phosphoglucomutase is a key enzyme in glycolysis and has been widely studied in vertebrates and some invertebrates but no molecular information is available in marine invertebrates despite the importance of this marker in ecological and genetical studies. In this work, we isolated a cDNA and the corresponding genomic sequence that encode PGM-2 locus in the Pacific oyster Crassostrea gigas. We used sequences drawn from the database to construct an evolutionary framework for examining the position of mollusc PGM sequences among prokaryotic and eukaryotic homologues and showed that oyster PGM gene organization was closer to vertebrates PGM genes than other invertebrates as previously found in other Lophotrochozoa species. We also investigated PGM mRNA...
Tipo: Text Palavras-chave: Gene expression; Xenobiotics; Mollusc; Phosphoglucomutase.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2220.pdf
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Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment ArchiMer
Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean.
We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Tipo: Text Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf
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Responses of the European flounder (Platichthys flesus) to a mixture of PAHs and PCBs in experimental conditions ArchiMer
Dupuy, Celie; Galland, Claire; Devaux, Alain; Bony, Sylvie; Loizeau, Veronique; Danion, Morgane; Pichereau, Vianney; Fournier, Michel; Laroche, Jean.
A multibiomarker approach was developed to evaluate the juvenile European flounder responses to a complex mixture of 9 polycyclic aromatic hydrocarbons (PAHs) and 12 polychlorinated biphenyls (PCBs). Exposure was performed through contaminated food pellets displaying: (1) PAH and PCB levels similar to those detected in the heavily polluted Seine estuary, respectively in sediments and in flatfish and (2) ten times these concentrations. Several biomarkers of the immune system (e.g., lysozyme concentration and gene expression of complement component C3 and TNF-receptor), DNA damage (e.g., Comet assay), energetic metabolism (e.g., activity of cytochrome C oxidase), detoxification process (e.g., cytochrome P450 1A1 expression level: CYP1A1; betaine homocysteine...
Tipo: Text Palavras-chave: Mixture of contaminants; Fish; Biomarker; Immunotoxicity; Detoxification process; DNA damage; PAHs; PCBs.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29914/28374.pdf
Registros recuperados: 9
Primeira ... 1 ... Última
 

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