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Registros recuperados: 11
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Calcein staining of calcified structures in pearl oyster Pinctada margaritifera and the effect of food resource level on shell growth ArchiMer
Linard, Clementine; Gueguen, Yannick; Moriceau, Jacques; Soyez, Claude; Hui, Belinda; Raoux, Aurore; Cuif, Jean Pierre; Cochard, Jean-claude; Le Pennec, Marcel; Le Moullac, Gilles.
Marine mollusc shell growth has been widely measured using fluorochrome marking. In order to test the efficiency and reliability of calcein staining on P. margaritifera shells and pearls, the present study examined two administration methods, different concentrations and several immersion times.Immersion in a 150 mg.L-1 calcein solution for 12 h to 24 h appeared to be the best method for marking P. margaritifera shells. For pearl marking, injection of a 200 mg.L-1 calcein solution into the pearl pouch was the optimal method. Calcein marking was then used to measure the influence of food resource levels on the shell growth. Groups of 23-month-old P. margaritifera were fed at three trophic levels for two months. The two highest food levels tested (6 000...
Tipo: Text Palavras-chave: Bivalvia; Pearl; Shell; Growth; Scanning electron microscope.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00028/13907/11195.pdf
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Comparative histological study of gametogenesis in diploid and triploid pacific oysters (Crassostrea gigas) reared in an estuarine farming site in France during the 2003 heatwave ArchiMer
Normand, Julien; Le Pennec, Marcel; Boudry, Pierre.
We compared the temporal dynamics of gametogenesis in diploid and triploid Pacific oysters (Crassostrea gigas) using histology. Oysters were reared in an estuarine farming site in Brittany over 3 years and their gametogenesis was monitored over the exceptionally hot summer of 2003. Both diploids and triploids showed active gametogenesis, but a high proportion of triploids remained at early stages of gonad development. Gametogenesis of triploids was characterized by the simultaneous occurrence of gonias and immature cytes together with mature gametes rather than overall retardation, though some triploids showed complete gonad maturation. Evidence of spawning was seen in both groups, and the quantity of germinal products emitted appeared to be similar in...
Tipo: Text Palavras-chave: Triploidy; Crassostrea gigas; Oyster; Gametogenesis.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4152.pdf
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Etude comparative du cycle de reproduction de la palourde Ruditapes decussatus en milieu naturel (sud Tunisie) et contrôlé (écloserie) ArchiMer
Hamida, Leila; Medhiouband, Mohamed; Cochard, Jean-claude; Romdhane, Mohamed; Le Pennec, Marcel.
A comparative study of the reproductive cycle of Ruditapes decussatus under natural (South of Tunisia) and controlled conditions (hatchery). The aim of this study was to describe and compare the gametogenic activity of Ruditapes decussatus in situ and in hatchery. Criteria of maturity were characterized and the positive role of conditioning in the success of artificial reproduction was underlined. Specimens sampled in the South of Tunisia were conditioned at the experimental station of Monastir (Tunisia). The parameters studied were the variation of the condition index and the frequency distribution of oocyte diameters. Results showed that the reproductive cycle was continuous in situ. Gametogenesis started in January and February. Gonadic activity...
Tipo: Text Palavras-chave: Ruditapes; Natural conditions; Hatchery; Reproduction; Gonatic activity; Gametogenesis.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-882.pdf
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Gamete cryopreservation, an asset for a durable pearl farming in French Polynesia ArchiMer
Hui, Belinda; Demoy-schneider, Marina; Vonau, Vincent; Le Moullac, Gilles; Moriceau, Jacques; Le Pennec, Marcel; Cochard, Jean-claude.
Cryopreservation is a useful tool for genetic improvement which has been applied to several bivalve mollusk species. It would allow to keep the gametes of selected individuals and to preserve ex-situ the biodiversity of wild populations of the black-lip pearl oyster Pinctada margaritifera threatened of standardization by the seed transfers between the islands in French Polynesia. The ability to cryopreserve spermatozoa would bring about significant benefits to the cultured black pearl industry. Sperm freezing supposes the control of different steps: preparation of breeders, sperm collection, evaluation of sperm quality and the freezing process itself. Broodstock conditioning in hatchery allows to obtain gametes from the pearl oyster. Sperm quality is...
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00029/14043/11238.pdf
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Influence of plankton concentration on gametogenesis and spawning of the black lip pearl oyster Pinctada margaritifera in Ahe atoll lagoon (Tuamotu archipelago, French polynesia) ArchiMer
Fournier, Jonathan; Levesque, Emmanuelle; Pouvreau, Stephane; Le Pennec, Marcel; Le Moullac, Gilles.
Pearl culture industry represents one of the dominant business sector of French Polynesia. However, it still entirely relies on unpredictable spat collection success. Our aim was to assess the influence of natural plankton concentration fluctuations on maturation and spawning of the black lip pearl oyster Pinctada margaritifera, during a 4 months survey conducted in Ahe atoll lagoon. Plankton concentration was assessed by chlorophyll a extraction and by microscope counts while gonadic index, gonado-visceral dry weights and histology were used to measure pearl oysters reproduction activity. We found that (i) plankton concentration fluctuations were mainly related to wind regime, (ii) gametogenesis rate was mainly related to plankton concentration, (iii)...
Tipo: Text Palavras-chave: Pearl Oyster; Gametogenesis; Spawning; Phytoplankton; French Polynesia.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00101/21201/19697.pdf
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Multi-scale distribution and dynamics of bivalve larvae in a deep atoll lagoon (Ahe, French Polynesia) ArchiMer
Thomas, Yoann; Garen, Pierre; Bennett, Auguste; Le Pennec, Marcel; Clavier, Jacques.
Bivalve larvae and hydrographic parameters were sampled over a range of spatio-temporal scales in a deep atoll lagoon. Bivalve larvae abundances were very high throughout the year: 18,550 m(-3) in average. Larvae were (i) concentrated at mid-depth with nocturnal ascent and diurnal descent, (ii) heterogeneously dispersed at the lagoon scale, (iii) subject to day-to-day variation in abundance and (iv) transferred between different parts of the lagoon providing evidence of intra-lagoonal connectivity. The primacy of physical factors was seen on large spatial scale with the diluting effect of water renewal and transfers by hydrodynamics. On smaller spatial scale, the primacy of biological processes was recognised, with larval swimming activity leading to dial...
Tipo: Text Palavras-chave: Bivalve larvae; Distribution patterns; Vertical migration; Pinctada margaritifera settlement; Deep atoll lagoon; French Polynesia.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00101/21200/20690.pdf
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Obtention de souches conchylicoles performantes par polyploïdisation (5ème partie) ArchiMer
Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Baud, Jean-pierre; Nourry, Max; Haure, Joel; Heurtebise, Serge; Garnier, Jacqueline; Martin, Anne-genevieve; Claude, Serge; Langlade, Aime; Joly, Jean-pierre; Goulletquer, Philippe; Ruelle, Francois; Coatanea, Denis; Oheix, Jocelyne; Vercelli, Catherine; Chabirand, Jean-michel; Maurer, Daniele; Borel, Michel; Cardinal, Mireille; Cornet, Josiane; Le Pennec, Marcel; Le Mercier, A..
This fifth part of the polyploidisation program reports the experiments that were conducted in 1993 and that were devoted to : - monitoring growth performance of Ostrea edulis diploids and triploids in La Trinité sur Mer and Palavas - monitoring growth performance of Crassostrea gigas diploids and triploids in Port en Bessin, Marennes Oleron (2 locations), Arcachon and Palavas - comparative study of gametogenesis for C. gigas diploids and triploids - comparative study of C. gigas diploids and triploids using sensorial analysis At the end of fifth year, it was clear that triploids are advantageous although such a statement must be moderated according to locations. The gametogenesis stydy showed that triploidy is associated with partial to complete...
Tipo: Text Palavras-chave: Crassostrea gigas; Ostrea edulis; Cytogenetics; Polyploidy; Crassostra gigas; Ostrea edulis; Cytogénétique; Polyploïdie.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/rapport-3860.PDF
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Production en écloserie de l'huître océanienne Saccostrea cucullata echinat a (Quoy et Gaymard, 1835) ; recherches sur la morphogénèse de la charnière ArchiMer
Le Pennec, Marcel; Coatanea, Denis.
La production en écloserie de l'huître de roche Saccostrea cucullata echinata été réalisé en Polynésie française au Centre Océanologique du Pacifique (Taravao, Tahiti), à partir de géniteurs importés de Nouvelle-Calédonie. La morphogenèse de la charnière a été étudiée au microscope électronique à balayage depuis la naissance des denticules chez la prodissoconque II jusqu'à leur régression chez la jeune dissoconque. Ces observations montrent l'originalité de la charnière de cette espèce et permettent de confirmer la détermination du nouveau genre Saccostrea au sein de la famille des Ostreidae. Elles montrent aussi que la charnière primitive de Saccostrea se rapproche de celle d'Ostrea, ce qui permet de les situer à une place voisine dans le schéma de la...
Tipo: Text
Ano: 1985 URL: http://archimer.ifremer.fr/doc/00150/26165/24260.pdf
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Relations entre le contenu lipidique des ovocytes de Pecten maximus (Mollusque, bivalve) et les performances des larves en élevage ArchiMer
Le Pennec, Marcel; Gueguen,.
The lipid content of spawned oocytes of Pecten maximus was studied by image analysis of semi -thin histological sections and a lipid index was defined. The female gametes were obtained by thermic stimulations of mature adults scallops from different populations after out of season conditioning or sampled in situ during the spawning season. After fertilization, the larval development was followed. The results reveal a large variability in lipid content of normal oocytes. The lipid content can be regarded as homogeneous inside a spawning but the heterogeneity is greater for wild scallops than for conditioned ones. A strong relationship appears between the lipid index and the hatching success, and the number of abnormal larvae after two days rearing. But no...
Tipo: Text Palavras-chave: Mollusques; Bivalves; Pecten maximus; Ovocytes; Lipides; Elevages larvaires; Mollusc; Bivalve; Pecten maximus; Oocytes; Lipids; Larval development.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00022/13345/10379.pdf
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Temperature and photoperiod drive Crassostrea gigas reproductive internal clock ArchiMer
Fabioux, Caroline; Huvet, Arnaud; Le Souchu, Pierrick; Le Pennec, Marcel; Pouvreau, Stephane.
This study examined the gametogenic cycle of Crassostrea gigas in controlled conditions over one year, with a focus on the initiation of gametogenesis. This work analysed also the role of temperature and photoperiod in the regulation of oyster reproduction. Broodstock were maintained in natural (NC), accelerated (AC) and perpetual winter (WC) conditions of temperature and photoperiod, with feeding ad libitum. Qualitative and quantitative analyses of the reproductive pattern were performed using biometric measurement approach, sex ratio determination, histology and a gonad filling index. Each experimental treatment led to different strategies for growth and resource allocation. The gametogenic cycle, appeared entirely modulated, accelerated or delayed, by...
Tipo: Text Palavras-chave: Temperature; Regulation; Gonad filling index; Gonial mitosis; Reproduction; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-826.pdf
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The limits of morphometric features for the identification of black-lip pearl oyster (Pinctada margaritifera) larvae ArchiMer
Paugam, A; D' Ollone, Christine; Cochard, Jean-claude; Garen, Pierre; Le Pennec, Marcel.
As with most cultivated bivalves, culture of the Tahitian pearl oyster Pinctada margaritifera is particularly dependent on the natural environment, especially for spat supply. The ability to track in real time the abundance and the development of pearl oyster larvae in the plankton would help optimize spat collection in atolls of French Polynesia. However no identification criteria are available for the larvae of several bivalves species present in the lagoons and it is not yet possible to specifically monitor pearl oyster larvae. The aim of this study is to determine the most pertinent morphological identification criteria, to specifically identify the larvae of P. margaritifera and differentiate them from those of three other abundant species: Pinctada...
Tipo: Text Palavras-chave: Morphological features; Larval identification; Pinctada margaritifera.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2531.pdf
Registros recuperados: 11
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