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Contrasted survival under field or controlled conditions displays associations between mRNA levels of candidate genes and response to OsHV-1 infection in the Pacific oyster Crassostrea gigas ArchiMer
Normand, Julien; Li, Ronghua; Quillien, Virgile; Nicolas, Jean-louis; Boudry, Pierre; Pernet, Fabrice; Huvet, Arnaud.
Pacific oyster Crassostrea gigas suffers from chronic or sporadic mortality outbreaks worldwide, resulting from infectious diseases and/or physiological disorders triggered by environmental factors. Since 2008, ostreid herpesvirus OsHV-1 μVar has been identified as the main agent responsible for mass mortality of juvenile oysters in Europe. Previous studies of genome-wide expression profiling have provided candidate genes that potentially contribute to genetically-based resistance to summer mortality. To assess their value in determining resistance to the juvenile mass mortality that has occurred in France since 2008, we analyzed the expression of 17 candidate genes in an experimental infection by OsHV-1 μVar, and in an in vivo field experiment. Individual...
Tipo: Text Palavras-chave: Molluscs; Gene expression profiling; Inhibitor of kappa B; Summer mortality; Ostreid herpesvirus.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00177/28823/27325.pdf
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Development of four EST-SSR multiplex PCRs in the Pacific oyster (Crassostrea gigas) and their validation in parentage assignment ArchiMer
Li, Ronghua; Li, Qi; Cornette, Florence; Degremont, Lionel; Lapegue, Sylvie.
We report four highly informative multiplex PCRs developed from twelve previously described ESTSSRs in Crassostrea gigas. We evaluated and validated these multiplex PCRs in twelve full-sib families. The average allelic richness and the polymorphism information content (PIC) were 11.1 and 0.811 respectively. The combined power of exclusion was greater than 99.99% using all four multiplex assays. A hundred and forty three tests of segregation ratios revealed 11 significant departures from expected Mendelian ratios. The frequency of null alleles was estimated as 4.9% of all the alleles segregating based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 97% of all offspring were unambiguously...
Tipo: Text Palavras-chave: Pacific oyster; Crassostrea gigas; Microsatellite; EST; Multiplex PCR; Parentage assignment.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00013/12419/9999.pdf
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