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Registros recuperados: 13
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Aspergillus niveus Blochwitz 4128URM: new source for inulinase production BABT
Souza-Motta,Cristina Maria de; Cavalcanti,Maria Auxiliadora de Queiroz; Porto,Ana Lúcia Figueiredo; Moreira,Keila Aparecida; Lima Filho,José Luiz de.
Aspergillus niveus Blochwitz 4128 URM isolated from sunflower rhizosphere demonstrated a new source of inulinase. The enzyme was produced in culture medium containing inulin as substrate in the concentrations: 10, 15 and 20g L-1. Maximum enzyme activity was obtained in medium containing 20g L-1 inulin. The enzyme was partially purified using ammonium sulphate precipitation, followed by ion charge (DE-32) and molecular exclusion (Sephadex) chromatography. The results showed the optimal pH and temperature of inulinase from crude extract were 4.0 and 4.8 and 45ºC, respectively. The enzyme was purified 34.65 fold with yield of 53.63%. A. niveus 4128URM can be used in the inulinase production with use in the food industries.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niveus; Rhizosphere; Inulinase; Inulin; Purification.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300003
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Avaliação de variáveis que influenciam a hidrólise enzimática da caseína do leite de cabra Moxotó PAB
Lira,Tatiana Barros Ferreira; Bezerra,Vilma Sobral; Silva,Flávio de Oliveira; Dias,Giselle Maria Pereira; Lima Filho,José Luiz de; Porto,Tatiana Souza; Porto,Ana Lúcia Figueiredo.
O objetivo deste trabalho foi avaliar os efeitos da temperatura, pH, relação enzima:substrato (E:S) e tempo de reação sobre a hidrólise enzimática da caseína do leite de cabra Moxotó, com uso de diferentes enzimas proteolíticas. A hidrólise enzimática da caseína caprina foi realizada com uso de tripsina, pepsina e papaína. Para cada enzima, foram utilizados dois níveis de cada variável, na avaliação de seus efeitos sobre a hidrólise da caseína, em um fatorial completo 2(4). Os produtos da hidrólise foram visualizados em eletroforese SDS-PAGE. O melhor valor do grau de hidrólise com a enzima papaína foi obtido em pH 6,5, E:S de 1:150 e 5 horas de hidrólise a 50ºC (28,17%); com a tripsina, em pH 8,5, E:S de 1:150 e 5 horas a 40ºC (29,55%); e com a pepsina,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capra hircus; Leite de cabra; Papaína; Pepsina; Proteólise; Tripsina.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000900014
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Biotechnological richness of the northeastern semi-arid region: antioxidant activity of casein hydrolysates from Moxotó goat milk (Capra hircus Linnaeus, 1758) obtained by papain action Ciênc. Tecnol. Aliment.
Bezerra,Vilma Sobral; Campos,Julia Furtado; Silva,Roberto Afonso da; Porto,Tatiana Souza; Lima Filho,José Luiz de; Porto,Ana Lúcia Figueiredo.
This study aimed to identify antioxidant peptides from caprine casein hydrolysates by papain application using MALDI-TOF mass spectrometer, and a 2² full factorial design, with 4 axial points, in order to evaluate kinetic parameters (time and pH) effects on the degree of hydrolysis as well as the antioxidant activity of Moxotó goat milk casein peptides. Degree of hydrolysis was determined by total and soluble protein ratio in casein. Antioxidant activity was measured by ABTS method with 2, 2-cation-azinobis (3-ethylbenzothiazoline-6-sulfonic acid). TROLOX was used as standard. Peptide pattern and sequence of antioxidant amino acids were obtained using MALDI-TOF/MS. The highest degree of hydrolysis (28.5%) and antioxidant activity (2329.6 mmol.L TROLOX. mg-...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioactive peptides; Caprine casein; Antioxidant activity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000300020
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Collagenolytic enzymes produced by fungi: a systematic review BJM
Wanderley,Maria Carolina de Albuquerque; Duarte Neto,José Manoel Wanderley; Lima Filho,José Luiz de; Lima,Carolina de Albuquerque; Teixeira,José António Couto; Porto,Ana Lúcia Figueiredo.
Abstract Specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic OR collagenase) AND (fungi OR fungus OR fungal) AND (production OR synthesis OR synthesize) AND (characterization). Scientific criteria were adopted in this review to classify found articles by score (from 0 to 10). After exclusion criteria, 21 articles were selected. None obtained the maximum of 10 points defined by the methodology, which indicates a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Collagenase; Fungus; Characterization; Purification; Production.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100013
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Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles BABT
Bruno,Laura Maria; Lima Filho,José Luiz de; Castro,Heizir Ferreira de.
Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activities were found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida lipase; Mucor lipase; Hybrid matrix; Immobilization.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500003
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Immobilization of lipase from Fusarium solani FS1 BJM
Knight,Kirsty; Pimentel,Maria do Carmo Barros; Morais,Marcia Maria Camargo de; Ledingham,William M.; Lima Filho,José Luiz de; Maia,Maria de Mascena Diniz.
Lipase from Fusarium solani FS1 was immobilized by covalent attachment to polyacrylamide beads and onto magnetized Dacron, retaining 12% and 97% of activity, respectively. Lipase was also entrapped within polyacrylamide beads, retaining 53% of activity. Investigations of the kinetic characteristics of the immobilized derivatives using triolein as substrate showed that lipase immobilized onto polyacrilamide beads and Dacron did not follow Michaelis-Menten kinetics.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Fusarium solani FS1; Immobilized lipase; Enzyme kinetics; Dacron; Polyacrylamide beads.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300013
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PARTIAL CHARACTERIZATION OF PROTEASES FROM STREPTOMYCES CLAVULIGERUS USING AN INEXPENSIVE MEDIUM BJM
Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Duarte,Helena Simões; Tambourgi,Elias Basile; Melo,Eduardo Henrique Magalhães de; Silva,Valdinete Lins; Porto,Ana Lúcia Figueiredo; Lima Filho,José Luiz de.
The partial characterization of extracellular proteases from Streptomyces clavuligerus NRRL 3585 and 644 mutant was investigated. The enzyme production was carried out in batch fermentation using soy bean filtrate as nitrogen source. Maximum activity was obtained after 96h of fermentation with an initial pH of 7.0. The enzyme was partially purified by ammonium sulphate precipitation. Enzymes from the two strains retained 37% of their initial activities at pH 8.0 after 2 h incubation at 25ºC. Enzyme half-life at pH 8.0 and 60ºC was 40.30 and 53.32 min, respectively for both strains (partially purified extract). The optimum pH was obtained at pH 7.0-8.0 and 8.4 for enzymes produced for 3585 and 644 strains (crude extract), respectively, and 8.4 and 8.0 for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces clavuligerus; Extracellular proteases; Thermal stability; PH.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300010
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Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1 Rev. Microbiol.
Maia,Maria de Mascena Diniz; Morais,Marcia Maria Camargo de; Morais Jr.,Marcos Antonio de; Melo,Eduardo Henrique Magalhães; Lima Filho,José Luiz de.
A Brazilian strain of Fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.l-1 of lipase after 72 hours of cultivation at 25oC in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. Glucose (1% w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30oC and a good enzyme stability (80% activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50oC. Lipase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme kinetics; Ester hydrolysis; Extracellular lipase; Fusarium solani.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400003
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Proteínas do plasma seminal de caprinos relacionadas com o índice pluviométrico e a qualidade do sêmen Ciência Rural
Souza,Andreia Fernandes de; Leitão,Maria da Conceição Gomes; Batista,André Mariano; Porto,Ana Lúcia Figueiredo; Lima Filho,José Luiz de; Guerra,Maria Madalena Pessoa.
O objetivo deste estudo foi identificar as proteínas do plasma seminal de caprinos da raça Alpina Americana criados na região Nordeste do Brasil que estão relacionadas ao índice pluviométrico e à qualidade do sêmen. O sêmen foi obtido pelo método de vagina artificial a partir de três reprodutores e foi avaliado quanto aos parâmetros macroscópicos e microscópicos. O perfil de proteínas do plasma seminal foi realizado por eletroforese bidimensional. Os parâmetros volume do sêmen, integridade do acrossoma e proteínas totais evidenciaram diferença significativa (P<0,05) entre os períodos de alto (1,7mL, 90,3% e 372µg mL-1, respectivamente) e baixo (1,2mL, 80,3% e 494µg mL-1, respectivamente) índice pluviométrico. Foram detectados, nos períodos de alto e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capra hircus; Chuvas; Características seminais.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000400030
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Protoplast formation and regeneration from Streptomyces clavuligerus NRRL 3585 and clavulanic acid production BJM
Carneiro-da-Cunha,Maria das Graças; Lima Filho,José Luiz de; Campos-Takaki,Galba Maria de.
Protoplasts of the wild type Streptomyces clavuligerus NRRL 3585 (ATCC 27064) were formed from spores cultures obtained in the lag, exponential and stationary growth phases by using 0.5% glycine in the culture medium. The protoplasts were obtained by treatment of the cells with lysozyme (EC-3.2.1.17) 40,000 U (1mg/mL), in an osmotic solution for 90 min at 28ºC. The frequency of regenerated protoplasts in the lag phase was 1.7x10³ CFU/mL (28.97%), in the beginning of the exponential phase 0.4x10² CFU/mL (31.67%), in the exponential growth phase 2.5x10³ CFU/mL (46.30%) and 1.0x10(5) CFU/mL in stationary phase (48.45%). Antibiotic production and activity of regenerated protoplasts were observed in all phases, except in the lag phase. The protoplast formation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces clavuligerus; Protoplast; Clavulanic acid.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400014
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Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography BABT
Moreira,Keila Aparecida; Diogo,Margarida; Prazeres,Duarte Miguel; Lima Filho,José Luiz de; Porto,Ana Lúcia Figueiredo.
This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded nucleic acid impurities as compared with double-stranded plasmid DNA.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene therapy; Hydrophobic interaction chromatography; Plasmid; Purification.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400014
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Study of carbohydrates present in the cladodes of Opuntia ficus-indica (fodder palm), according to age and season Ciênc. Tecnol. Aliment.
Ribeiro,Erika Maria de Oliveira; Silva,Nicácio Henrique da; Lima Filho,José Luiz de; Brito,Júlio Zoe de; Silva,Maria da Paz Carvalho da.
Types and content of carbohydrates were evaluated by chemical (spectrophotometric assay) and physicochemical (Thin Layer Chromatography - TLC and High Performance Liquid Chromatography - HPLC) methods in some Opuntia ficus-indica varieties according to age and season. The samples comprised four varieties of palm (giant, copena F1, clone 20, and round palm). The results demonstrated that the four varieties of palm contain a good quantity of neutral and acid sugars in both summer and winter seasons. However, samples collected in the summer presented a higher content of carbohydrates specially glucose, fructose, galactose, xylose, and arabinose. The tertiary cladode (old cladode) presented almost the double content of sugar found in the quaternary cladodes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Opuntia ficus-indica; Carbohydrates; Pectin.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000400015
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Utilização de penas de galinha para produção de queratinase por Aspergillus carbonarius PAB
Sales,Marilia Ribeiro; Cavalcanti,Maria Taciana Holanda; Lima Filho,José Luiz de; Motta,Cristina Maria de Souza; Porto,Ana Lúcia Figueiredo.
O objetivo deste trabalho foi estudar a produção de queratinase por Aspergillus carbonarius URM 1546, tendo-se como substrato penas de galinha, por meio de planejamento fatorial completo 2³. Todos os parâmetros estudados e as interações de segunda ordem foram estatisticamente significativos. A maior atividade queratinolítica (48,9 U mL-1) foi obtida com 120 rpm, 0,5% (p/v) de penas de galinha e sete dias de cultivo.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzimas queratinolíticas; Fungos filamentosos; Queratina.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000200020
Registros recuperados: 13
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