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Combining transcriptome analyses and virus induced gene silencing to identify genes in the Rpp4-mediated asian soybean rust resistance Pathway. Repositório Alice
MORALES, A.; O'ROURKE, J. A.; BORÉM, A.; ABDELNOOR, R. V.; PEDLEY, K. F.; WHITHAM, S. A.; GRAHAM, M. A..
Six Asian Soybean Rust (ASR) resistance loci have been identified and mapped in soybean genome: Rpp1 (Resistance to Phakopsora pachyrhizi 1), Rpp2, Rpp3, Rpp4, Rpp5 and Rpp6. Of particular interest is Rpp4, which has remained stable and confers resistance against Phakopsora pachyrhizi isolates from around the world. Sequencing of the region harboring Rpp4 in the susceptible cultivar Williams 82 (Wm82) and the resistant cultivar (PI459025B) genotype identified a cluster of CC-NBS-LRR resistance genes. Meyers et al. (2009) developed Virus Induced Gene Silencing constructs from the LRR regions of the Wm82 Rpp4 candidate genes to confirm that orthologous genes were responsible for resistance in the resistant parent (PI459025B). In this study, RNA samples...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Soja; Doença de planta; Ferrugem; Soybeans; Rust diseases; Plant diseases and disorders; Phakopsora pachyrhizi.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/940523
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Phenolic compounds accumulation in soybean plants in response to Phakopsora pachyrhizi. Repositório Alice
MORALES, A.; ALAN ALVES PEREIRA; CATELLI, L.; BORÉM, A.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, M. C. N. de; MICHELLE A. GRAHAM; HOFFMANN-CAMPO, C. B.; ABDELNOOR, R. V..
Asian soybean rust (ASR) is a soybean disease caused by Phakopsora pachyrhizi Sydow. The phenylpropanoid pathway is involved in many biological processes including the defense response to the fungus. This pathway results in the production of lignin and phytoalexins, which are an important defense against pathogens and insects. In the present work, we used high performance liquid chromatography (HPLC) to measure the production of compounds in the phenylpropanoid pathway in leaves of the resistant soybean genotype (PI459025B, Rpp4). Plants were either infected with P. pachyrhizi or mock infected with water, and leaf tissue was collected at 24, 48, 72, 96, 120, 161, 308 and 504 hours after inoculation (hai). Phenolic peak quantification was carried out by...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Soja; Doença de planta; Ferrugem; Soybeans; Plant diseases and disorders; Rust diseases; Phakopsora pachyrhizi.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/940515
Registros recuperados: 2
Primeira ... 1 ... Última
 

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