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Registros recuperados: 13
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20-Hydroxyecdysone deters oviposition and larval feeding in the European grapevine moth, Lobesia botrana Inra
Calas, D.; Thiery, D.; Marion-Poll, F..
European grapevine moth females (Lobesia botrana, Lepidoptera Tortricidae) select an oviposition site by tasting the host plant surface and then gluing a single egg on berries from grapes or from several other host plant species. In doing so, females should avoid ovipositing on plants that are detrimental to their progeny. Do they sense the same deterrent compounds as larvae, despite the fact that they do not have access to the same compartments of the plants? We tested this hypothesis with 20-hydroxyecdysone (20E), purified from Leuzea carthamoides. Phytoecdysteroids are usually found inside plant tissues and accessible to larvae in an aqueous phase, while adults would access them only through the epicuticular wax. We first confirmed that larvae avoid...
Tipo: Journal Article Palavras-chave: LOBESIA BOTRANA.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20079b9fdcb5&uri=/notices/prodinra1/2010/11/
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Discrimination of oilseed rape volatiles by honey bee: novel combined gas chromatographic-electrophysiological behavioral assay Inra
Wadhams, L.J.; Blight, M.M.; Kerguelen, V.; Le Metayer, M.; Marion-Poll, F.; Masson, C.; Pham-Delegue, M.H.; Woodcock, C.M..
A novel technique for the simultaneous monitoring of electroan-tennogram (EAG) and conditioned proboscis extension (CPE) responses of honey bees to the effluent from a gas chromatograph (GC) was developed to locate biologically active components in blends of plant volatiles and to investigate odor recognition at the peripheral and behavioral levels. A six-component mixture, comprising compounds previously identified as oilseed rape floral volatiles, was used as the stimulus. Standard CPE and EAG recordings were done as a reference. EAG responses were elicited from unconditioned bees by all the components presented either in the coupled or the standard mode. Conditioned bees gave larger EAG responses than unconditioned bees, suggesting that antennal...
Tipo: Journal Article Palavras-chave: EXTENSION DU PROBOSCIS; REFLEXE CONDITIONNE; APIS MELLIFERA; APIDAE; DISCRIMINATION OLFACTIVE; ODEUR; ELECTROANTENNOGRAPHIE; CHROMATOGRAPHIE EN PHASE GAZEUSE; TEST BIOLOGIQUE HONEY BEE; APIS MELLIFERA; HYMENOPTERA; APIDAE; GAS CHROMATOGRAPHY; ELECTROANTENNOGRAM; CONDITIONED PROBOSCIS EXTENSION; OLFACTORY DISCRIMINATION.
Ano: 1994 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500025144043436&uri=/notices/prodinra1/2010/11/
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Do european corn borer females detect and avoid laying eggs in the presence of 20-hydroxyecdysone? Inra
Calas, D.; Berthier, A.; Marion-Poll, F..
European corn borer larvae detect and avoid feeding in the presence of phytoecdysteroids (PEs) such as 20-hydroxyecdysone (20E). Therefore, we hypothesized that females would have taste receptors similar to larvae and avoid laying eggs in the presence of 20E. We found female-specific taste sensilla on the tarsi that respond to 20E at concentrations as low as 10−6 M, a threshold comparable to that of larvae. However, in choice tests, females laid a similar number of eggs on 20E-treated and on nontreated artificial substrates (filter paper, glass, and nylon), although they spent significantly more time in behavioral sequences related to substrate assessment when 20E was present. In contrast, when given a choice between maize plants (eight leaves) sprayed...
Tipo: Journal Article Palavras-chave: OSTRINIA NUBILALIS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20071e35a878&uri=/notices/prodinra1/2010/11/
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Floral volatiles of Tanacetum vulgare L. attractive to Lobesia botrana Den. et Schiff. females Inra
Gabel, B.; Thiery, D.; Suchy, V.; Marion-Poll, F.; Hradsky, P.; Farkas, P..
The European grapevine moth (EGVM), Lobesia botrana, is a major pest of grapes in Europe. Females are attracted to a nonhost plant: tansy (Tanacetum vulgare L.), which is a common weed in Slovakian vineyards. A steam distillate extract of tansy flowers was analyzed by means of a GC-EAG technique to screen constituents detected by the olfactory receptors of EGVM females. From more than 200 GC peaks, nine peaks corresponding to monoterpenoids released an EAG response in more than 70% of the females (N=15):p-cymene,d-limonene,α-thujene,α-thujone,β-thujone, thujyl alcohol, terpinene-4-ol, (Z)-verbenol, and piperitone. The steam distillate of tansy as well as a synthetic blend of identified compounds released consistent attraction in a field cage. The use of...
Tipo: Journal Article Palavras-chave: LOBESIA BOTRANA; TANACETUM VULGARE; TANAISIE; LEPIDOPTERA; TORTRICIDAE; ATTRACTION; DEPREDATEUR; CHROMATOGRAPHIE EN PHASE GAZEUSE; SPECTROMETRIE DE MASSE; ELECTROANTENNOGRAPHIE; MEDIATEUR CHIMIQUE; TERPENOIDE LOBESIA BOTRANA; VITIS VINIFERA; TANACETUM VULGARE; TANSY; GRAPEVINE; LEPIDOPTERA; TORTRICIDAE; COMPOSITAE; PLANT-INSECT RELATIONSHIPS; OLFACTION; ALLELOCHEMICALS; TERPENOIDS; COUPLING GC-EAG.
Ano: 1992 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB920002500600028663&uri=/notices/prodinra1/2010/11/
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Hedonic taste in Drosophila revealed by olfactory receptors expressed in taste neurons Inra
Hiroi, M.; Tanimura, T.; Marion-Poll, F..
Taste and olfaction are each tuned to a unique set of chemicals in the outside world, and their corresponding sensory spaces are mapped in different areas in the brain. This dichotomy matches categories of receptors detecting molecules either in the gaseous or in the liquid phase in terrestrial animals. However, in Drosophila olfactory and gustatory neurons express receptors which belong to the same family of 7-transmembrane domain proteins. Striking overlaps exist in their sequence structure and in their expression pattern, suggesting that there might be some functional commonalities between them. In this work, we tested the assumption that Drosophila olfactory receptor proteins are compatible with taste neurons by ectopically expressing an olfactory...
Tipo: Journal Article Palavras-chave: NEURONE; RECEPTEUR; PROTEINE; SENSATION; GOUT; FONCTION PHYSIOLOGIQUE; INSECTE; ARTHROPODA; HEXAPODA; STIMULUS; SUCRE; CAFEINE  OLFACTION; NEURON; PROTEIN RECEPTOR; TASTE; DROSOPHILA.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201058c69fd&uri=/notices/prodinra1/2010/10/
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Identification of floral volatiles involved in recognition of oilseed rape flowers, Brassica napus by honeybees, Apis mellifera Inra
Blight, M.M.; Le Métayer, M.; Pham-Delègue, M.H.; Pickett, J.A.; Marion-Poll, F.; Wadhams, L.J..
Volatiles from oilseed rape, Brassica napus, flowers were sampled by air entrainment and their relevance to the natural odor profile of the flowers was confirmed by conditioned proboscis extension (CPE) assays with honeybee, Apis mellifera L., foragers. Coupled gas chromatography (GC)-CPE analysis of the air entrainment samples was used to locate key compounds involved in the recognition of B. napus flowers, and the compounds were then identified using coupled gas chromatography-mass spectrometry and comparison with authentic samples. Six regions of the gas chromatograms elicited CPE responses from bees previously conditioned to the total extract, and from these areas 16 compounds were identified that elicited CPE activity from conditioned bees when tested...
Tipo: Journal Article Palavras-chave: HYMENOPTERE; MEMOIRE OLFACTIVE; APIS MELLIFERA; INSECTE SOCIAL; CHROMATOGRAPHIE EN PHASE GAZEUSE; SPECTROMETRIE DE MASSE; SYNTHESE CHIMIQUE; ABEILLE DOMESTIQUE HONEYBEE; APIS MELLIFERA; HYMENOPTERA; APIDAE; BRASSICA NAPUS; OILSEED RAPE; FLOWER VOLATILES; CONDITIONED PROBOSCIS EXTENSION; OLFACTORY RECOGNITION.
Ano: 1997 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9900008399078149&uri=/notices/prodinra1/2010/11/
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Parallel reinforcement pathways for conditioned food aversions in the honeybee Inra
Wright, G.A.; Mustard, J.A.; Simcock, N.K.; Ross-Taylor, A.A.R.; McNicholas, L.D.; Popescu, A.; Marion-Poll, F..
Avoiding toxins in food is as important as obtaining nutrition. Conditioned food aversions have been studied in animals as diverse as nematodes and humans [1, 2], but the neural signaling mechanisms underlying this form of learning have been difficult to pinpoint. Honeybees quickly learn to associate floral cues with food [3], a trait that makes them an excellent model organism for studying the neural mechanisms of learning and memory. Here we show that honeybees not only detect toxins but can also learn to associate odors with both the taste of toxins and the postingestive consequences of consuming them. We found that two distinct monoaminergic pathways mediate learned food aversions in the honeybee. As for other insect species conditioned with salt or...
Tipo: Journal Article Palavras-chave: INSECTE; INSECTE UTILE; ALIMENTATION; TOXINE; APPRENTISSAGE; MEMOIRE; ODEUR; OLFACTION; DOPAMINE; SEROTONINE; SYSTME NERVEUX; ABEILLE DOMESTIQUE .
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201034c88ffd&uri=/notices/prodinra1/2011/05/
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Peripheral, Central and Behavioral Responses to the Cuticular Pheromone Bouquet in Drosophila melanogaster Males Inra
Inoshita, T.; Martin, J.R.; Marion-Poll, F.; Ferveur, J.F..
Pheromonal communication is crucial with regard to mate choice in many animals including insects. Drosophila melanogaster flies produce a pheromonal bouquet with many cuticular hydrocarbons some of which diverge between the sexes and differently affect male courtship behavior. Cuticular pheromones have a relatively high weight and are thought to be — mostly but not only — detected by gustatory contact. However, the response of the peripheral and central gustatory systems to these substances remains poorly explored. We measured the effect induced by pheromonal cuticular mixtures on (i) the electrophysiological response of peripheral gustatory receptor neurons, (ii) the calcium variation in brain centers receiving these gustatory inputs and (iii) the...
Tipo: Journal Article Palavras-chave: DROSOPHILA MELANOGASTER; MALES; CUTICULAR PHEROMONE BOUQUET; PHEROMONAL COMMUNICATION; INSECTS; CUTICULAR HYDROCARBONS; GUSTATORY CONTACT; PERIPHERAL GUSTATORY RECEPTOR NEURONS; CALCIUM VARIATION; MUTANT DESAT1 MALES; ANOSMIC MALES; PHEROMONAL PERCEPTION.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011ccb52cef&uri=/notices/prodinra1/2011/06/
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Pulsed odors from maize or spinach elicit orientation in european corn borer neonate larvae Inra
Piesik, D.; Rochat, D.; van der Pers, J.; Marion-Poll, F..
Lepidoptera larvae are capable of orienting towards or away from plants by using odors as cues but whether this attraction is innate or secondarily acquired remains unknown. We tested the hypothesis that European corn borer (ECB) neonate larvae express an innate attraction towards odors released from maize, and avoidance towards odors from spinach. Neonate larvae were placed on a locomotion compensator within a constant stream of humidified air that was loaded intermittently with airborne odors drawn from potted plants. The odor stream was delivered continuously or pulsed (1 to 10 sec pulses) at 40 ml/min. ECB larvae oriented toward maize odors pulsed at 2 to 6 sec but walked away from maize odors delivered at lower frequencies (9 and 10 sec pulses or to...
Tipo: Journal Article Palavras-chave: ANALYSE STATISTIQUE; ATTRACTIF; LARVE; ODEUR; EPINARD; OLFACTION; INSECTE; LEPIDOPTERA; PYRALIDAE EUROPEAN CORN BORER; LEPIDOPTERA LARVAE; NEONATE LARVAE; OSTRINIA NUBILALIS; ZEA MAYDIS; SPINACEA OLEACEA; LOCOMOTION COMPENSATOR.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20102a65498a&uri=/notices/prodinra1/2010/11/
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Reconnaissance de composés volatils du colza (Brassica napus L var oleifera) par l'abeille Inra
Pham-Delegue, M.H.; Laloi, D.; Bailez, O.; Le Metayer, M.; Marion-Poll, F.; Blight, M.; Wadhams, L..
Tipo: Journal Article Palavras-chave: SIGNAL CHIMIQUE; EXTENSION DU PROBOSCIS; RECONNAISSANCE CHIMIQUE.
Ano: 1994 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500004430051757&uri=/notices/prodinra1/2009/12/
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Temporal coding of pheronome pulses and trains in Manduca sexta Inra
Marion-Poll, F.; Tobin, T.R..
We investigated the ability of pheromone-sensitive olfactory receptors of male Manduca sexta to respond to 20-ms pulses of bombykal, the major component of the conspecific pheromonal blend. Isolated pulses of bombykal elicited a burst of activity which decreased exponentially with a time constant of 160–250 ms. Trains of pulses delivered at increasing frequencies (0.5–10 Hz) elicited temporally modulated responses at up to 3 Hz. Concentration of the stimulus (1, 10, 100 ng per odor source) had a marginal effect on the temporal resolution of the receptors. Within a train, the responses to individual pulses remained constant, except for 10-Hz trains (short-term adaptation). A dose-dependent decline of responsiveness was observed during experiments (long-term...
Tipo: Journal Article Palavras-chave: LEPIDOPTERA; SPHINGIDAE; MANDUCA SEXTA; ELECTROPHYSIOLOGIE ANDUCA SEXTA; OLFACTION; PHEROMONES; TEMPORAL CODING; ADAPTATION.
Ano: 1992 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9300009635034872&uri=/notices/prodinra1/2010/11/
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Terpene variations in maritime pine constitutive oleoresin related to host tree selection by Dioryctria sylvestrella RATZ. (Lepidoptera : Pyralidae) Inra
Jactel, H.; Kleinhentz, M.; Marpeau-Bezard, A.; Marion-Poll, F.; Menassieu, P.; Burban, C..
Gas chromatographic analysis of the volatile terpenes of the wood oleoresin of maritime pines showed that the relative proportions of terpenes within the same tree did not vary according to sampling height and date. Pruning and tree vigor did not affect terpene profiles, but oleoresin composition varied widely from tree to tree. The trees attacked by Dioryctria sylvestrella contained a significantly higher percentage of limonene, longipinene, and copaene, and a discriminant analysis, using the relative percentage of six terpenes, significantly separated infested and uninfested trees. The olfactory sensitivity of adult moths to a series of 20 volatile chemicals, including maritime pine oleoresin components, was evaluated with the electroantennogram method....
Tipo: Journal Article Palavras-chave: DIORYCTRIA SYLVESTRELLA; PYRALE; LEPIDOPTERE; RECONNAISSANCE OLFACTIVE; TERPENE; ODEUR; DEPREDATEUR; ATTRACTION; RESISTANCE; SELECTION; MARQUEUR PINE TREE; PINUS PINASTER; LEPIDOPTERA; DIORYCTRIA SYLVESTRELLA; OLEORESIN; TERPENES; HOST SELECTION; RESISTANCE MARKER.
Ano: 1996 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9700011276061403&uri=/notices/prodinra1/2010/11/
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Water taste transduction pathway is calcium dependent in Drosophila Inra
Meunier, N.; Marion-Poll, F.; Lucas, P..
In mammals, detection of osmolarity by the gustatory system was overlooked until recently. In insects, specific taste receptor neurons detect hypoosmotic stimuli and are commonly called “W” (water) cells. W cells are easy to access in vivo and represent a good model to study the transduction of hypoosmotic stimuli. Using pharmacological and genetic approaches in Drosophila, we show that tarsal W cell firing activity depends on the concentration of external calcium bathing the dendrite. This dependence was confirmed by the strong inhibition of W cell responses to hypoosmotic stimuli by lanthanum (IC50 = 8 nM), an ion known to inhibit calcium-permeable channels. Downstream, the transduction pathway likely involves calmodulin because calmodulin antagonists...
Tipo: Journal Article Palavras-chave: DROSOPHILA; ARTHROPODA; HEXAPODA; INSECTE; NEURONE; RECEPTEUR; STIMULUS HYPOOSMOTIQUE; GOUT; SENSATION; EAU; CALCIUM; CALMODULINE; OSMOLARITE  CALMODULIN; HYPOOSMOTIC; INSECT; OSMOLARITY; PKC; TRANSDUCTION.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20104d76cadf&uri=/notices/prodinra1/2010/10/
Registros recuperados: 13
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