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A PCR-based method for diet analysis in freshwater organisms using rDNA barcoding on faeces Inra
Corse, E.; Costedoat, C.; Chappaz, R.; Pech, N.; Martin, J.F.; Gilles, A..
The development of DNA barcoding from faeces represents a promising method for animal diet analysis. However, current studies mainly rely on prior knowledge of prey diversity for a specific predator rather than on a range of its potential prey species. Considering that the feeding behaviour of teleosts may evolve with their environment, it could prove difficult to establish an exhaustive listing of their prey. In this article, we extend the DNA barcoding approach to diet analysis to allow the inclusion of a wide taxonomic range of potential prey items. Thirty-four ecological clade-specific primer sets were designed to cover a large proportion of prey species found in European river ecosystems. Selected primers sets were tested on isolated animal, algal or...
Tipo: Journal Article Palavras-chave: FECES; PREDATEURS; PROIES; ECOSYSTEMES FLUVIAUX; BASSIN HYDROGRAPHIQUE 18S RDNA; CYPRINIDAE; DNA BARCODING; FAECES; GROUP-SPECIFIC PRIMERS; STREAM FOOD WEB.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20118abaf491&uri=/notices/prodinra1/2011/06/
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Cross-species amplification of 41 microsatellites in European cyprinids: a tool for evolutionary,population genetics and hybridization studies Inra
Dubut, V.; Sinama, M.; Martin, J.F.; Meglécz, E.; Fernandez, J.; Chappaz, R.; Gilles, A.; Costedoat, C..
Background:Cyprinids display the most abundant and widespread species among the European freshwater Teleostei and are known to hybridize quite commonly. Nevertheless, a limited number of markers for conducting comparative differentiation, evolutionary and hybridization dynamics studies are available to date. Findings: Five multiplex PCR sets were optimized in order to assay 41 cyprinid-specific polymorphic microsatellite loci (including 10 novel loci isolated from Chondrostoma nasus nasus, Chondrostoma toxostoma toxostoma and Leuciscus leuciscus) for 503 individuals (440 purebred specimens and 63 hybrids) from 15 European cyprinid species. The level of genetic diversity was assessed in Alburnus alburnus, Alburnoides bipunctatus, C. genei, C. n. nasus, C....
Tipo: Journal Article Palavras-chave:  .
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20101eb6a2de&uri=/notices/prodinra1/2010/11/
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Eight polymorphic microsatellite loci for the Australian plague locust, Chortoicetes terminifera Inra
Chapuis, M.P.; Popple, J.A.; Simpson, S.J.; Estoup, A.; Martin, J.F.; Steinbauer, M.; McCulloch, L.; Sword, G.A..
Few population genetics studies have been carried out on major locust species. In particular, an understanding of the population genetic structure of the Australian plague locust, Chortoicetes terminifera, is lacking. We isolated and characterized eight polymorphic microsatellite loci in C. terminifera, and described experimental conditions for polymerase chain reaction multiplexing and genotyping these loci. The number of alleles per locus ranged from 11 to 29 and the expected heterozygosity ranged from 0.797 to 0.977. One locus was found to be X-linked. Results of cross-taxon amplification tests are reported in four species of the Oedipodinae subfamily
Tipo: Journal Article Palavras-chave: CRIQUET PELERIN; ESPECE INVASIVE CHORTOICETES TERMINIFERA; LOCUST; MICROSATELLITES; NULL ALLELES; ORTHOPTERA; PEST.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009f0f82bce&uri=/notices/prodinra1/2010/02/
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Isolation and characterization of microsatellites in the harlequin ladybird Harmonia axyridis (Coleoptera, Coccinellidae) and cross-species amplification within the family Coccinellidae Inra
Loiseau, A.; Malausa, T.; Lombaert, E.; Martin, J.F.; Estoup, A..
A total of 18 microsatellite DNA loci were isolated and characterized from the harlequin ladybird, Harmonia axyridis (Coleoptera: Coccinellidae). We optimized a multiplex panel consisting of two polymerase chain reactions, allowing the genotyping of all loci. The number of alleles and heterozygosity observed at each locus ranged from 1 to 12 and from 0 to 100%, respectively. After Bonferroni correction for multiple tests, none of the loci deviated significantly from Hardy-Weinberg equilibrium and there was no indication of significant linkage disequilibrium among pairs of loci. Successful cross-species amplification was obtained for only three of the seven tested species of Coccinellidae
Tipo: Journal Article Palavras-chave: COCCINELLE; COLEOPTERE; ESPECE INVASIVE; MICROSATELLITE BIOLOGICAL INVASION; INSECT; INVASIVE SPECIES; MICROSATELLITES; NUCLEAR MARKER.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200946b6d228&uri=/notices/prodinra1/2010/08/
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Molecular differentiation at nuclear loci in French host races of the European corn borer (Ostrinia nubilalis) Inra
Malausa, T.; Leniaud, L.; Martin, J.F.; Audiot, P.; Bourguet, D.; Ponsard, S.; Lee, S.F.; Harrison, R.G.; Dopman, E..
French populations of the European corn borer consist of two sympatric and genetically differentiated host races. As such, they are well suited to study processes that could be involved in sympatric speciation, but the initial conditions of host-race divergence need to be elucidated. Gene genealogies can provide insight into the processes involved in speciation. We used DNA sequences of four nuclear genes to (1) document the genetic structure of the two French host races previously delineated with allozyme markers, (2) find genes directly or indirectly involved in reproductive isolation between host races, and (3) estimate the time since divergence of the two taxa and see whether this estimate is compatible with this divergence being the result of a host...
Tipo: Journal Article Palavras-chave: PYRALE DU MAÏS; SPECIATION SYMPATRIQUE; POLYMORPHISME GENETIQUE OSTRINIA NUBILALIS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008292c351f&uri=/notices/prodinra1/2008/08/
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PCR-based amplification and analysis of specific viral sequences from individual plant cells Inra
Yvon, M.; Monsion, B.; Martin, J.F.; Gutiérrez, S.; Blanc, S..
Plant virus diversity and the spatial distribution of viral strains or isolates are studied at many different scales: global, regional, local, and even within a single host in different organs or tissues. However, one level that has been totally lacking at the extremity of this scale is that of the single cell. The technical difficulties involved in isolating individual cells from infected plants, and the lack of an efficient diagnostic procedure allowing the specific detection of viral sequences with no major contamination from other cells, have precluded such single cell analysis to date. This paper describes the preparation of protoplasts from plants infected with Cauliflower mosaic virus (CaMV), and their decontamination and separation using a...
Tipo: Journal Article Palavras-chave: DIVERSITE GENETIQUE PLANT VIRUS; SINGLE-CELL ANALYSIS; NESTED-PCR; HIGH-RESOLUTION-MELTING ANALYSIS; CO-INFECTION.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011410c6831&uri=/notices/prodinra1/2011/06/
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Phylogeny of the genus Aphis Linnaeus, 1758 (Homoptera: Aphididae) inferred from mitochondrial DNA sequences. Inra
Coeur d'Acier, A.; Jousselin, E.; Martin, J.F.; Rasplus, J.Y..
Aphis is the largest aphid genus in the world and contains several of the most injurious aphid pests. It is also the most reluctant aphid genus to any comprehensive taxonomic treatment: while most species are easily classified into "species groups" that form well defined entities, numerous species within these groups are difficult to tell apart morphologically and identification keys remain ambiguous and mostly rely on host plant affiliation. In this paper, we used partial sequences of COI/COII and CytB genes to reconstruct the first phylogeny of Aphis and discuss the present systematics. The monophyly of the subgenus Bursaphis and of the tree major species groups, Black aphid, Black backed aphid and frangulae-like species was recovered by all phylogenetic...
Tipo: Journal Article Palavras-chave: ADN MITOCHONDRIAL MOLECULAR PHYLOGENETICS; SYSTEMATIC; APHID; BURSAPHIS; APHIDINAE; APHIDINI; MITOCHONDRIAL DNA; SPECIES-GROUP; BLACK APHID; BLACK BACKED APHID; FRANGULAE-LIKE SPECIES APHID.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007a551d1ad&uri=/notices/prodinra1/2007/07/
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Representativeness of microsatellite distributions in genomes, as revealed by 454 GS-FLX Titanium pyrosequencing Inra
Martin, J.F.; Pech, N.; Meglécz, E.; Ferreira, S.; Costedoat, C.; Dubut, V.; Malausa, T.; Gilles, A..
Background : Microsatellites are markers of choice in population genetics and genomics, as they provide useful insight into patterns and processes as diverse as genome evolutionary dynamics and demographic processes. The acquisition of microsatellites through multiplex-enriched libraries and 454 GS-FLX Titanium pyrosequencing is a promising new tool for the isolation of new markers in unknown genomes. This approach can also be used to evaluate the extent to which microsatellite-enriched libraries are representative of the genome from which they were isolated. In this study, we deciphered potential discrepancies in microsatellite content recovery for two reference genomes (Apis mellifera and Danio rerio), selected on the basis of their extreme heterogeneity...
Tipo: Journal Article Palavras-chave: MICROSATELLITE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20108ec8cfc7&uri=/notices/prodinra1/2010/12/
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Social immunity in honeybees (Apis mellifera): transcriptome analysis of varroa-hygienic behaviour Inra
Le Conte, Y.; ALAUX, C.; Martin, J.F.; Harbo, J.R.; Harris, J.W.; Dantec, C.; Séverac, D.; Cros-Arteil, S.; Navajas Navarro, M..
Honeybees have evolved a social immunity consisting of the cooperation of individuals to decrease disease in the hive. We identified a set of genes involved in this social immunity by analysing the brain transcriptome of highly varroa-hygienic bees, who efficiently detect and remove brood infected with the Varroa destructor mite. The function of these candidate genes does not seem to support a higher olfactory sensitivity in hygienic bees, as previously hypothesized. However, comparing their genomic profile with those from other behaviours suggests a link with brood care and the highly varroa-hygienic Africanized honeybees. These results represent a firststep toward the identification of genes involved in social immunity and thus provide first insights...
Tipo: Journal Article Palavras-chave: PARASITE; INSECTE SOCIAL; IMMUNITE SOCIALE; COMPORTEMENT HYGIENIQUE; EXPRESSION GENIQUE HONEYBEE; SOCIAL IMMUNITY; HYGIENIC BEHAVIOUR; VARROA; GENE EXPRESSION.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011f6e141fc&uri=/notices/prodinra1/2011/06/
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The effect of pathogens and pests on honey bee gene expression Inra
Navajas Navarro, M.; Martin, J.F.; Le Conte, Y.; Queen's University Belfast (GBR).
The effect of pathogens and pests on honey bee gene expression is a fascinating area of research initself and can lead to new molecular tools for diagnostics and selection in beekeeping. In thisframework, we first investigated Varroa-bee interactions by using a combination of nuc1ear andmitochondrial DNA markers of Varroa destructor to trace the parasite invasion of Apis melliferasince it shifted from A. cerana. The extremely low worldwide mite genetic diversity found on A.mellifera outside Asia contrasts with the variety of known bee phenotypes regarding their response tothe parasite. This prompted us to further explore the response of bees to Varroa, by using atranscriptional study. We compared parasitized and non-parasitized full-sister pupae, from...
Tipo: Conference Paper Palavras-chave:  INSECTE SOCIAL;  POLLINISATEUR;  EXPRESSION GENIQUE;  GENOTYPE;  PARASITE;  PUCE ADN;  MICROARRAY  APIS MELLIFERA;  APIDAE;  APIFORMES;  HYMENOPTERA;  VARROA DESTRUCTOR;  ACARI;  MESOSTIGMATA.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20092388b04&uri=/notices/prodinra1/2009/06/
Registros recuperados: 10
Primeira ... 1 ... Última
 

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