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Biopharmaceuticals from microorganisms: from production to purification BJM
Jozala,Angela Faustino; Geraldes,Danilo Costa; Tundisi,Louise Lacalendola; Feitosa,Valker de Araújo; Breyer,Carlos Alexandre; Cardoso,Samuel Leite; Mazzola,Priscila Gava; Oliveira-Nascimento,Laura de; Rangel-Yagui,Carlota de Oliveira; Magalhães,Pérola de Oliveira; Oliveira,Marcos Antonio de; Pessoa Jr,Adalberto.
ABSTRACT The use of biopharmaceuticals dates from the 19th century and within 5-10 years, up to 50% of all drugs in development will be biopharmaceuticals. In the 1980s, the biopharmaceutical industry experienced a significant growth in the production and approval of recombinant proteins such as interferons (IFN α, β, and γ) and growth hormones. The production of biopharmaceuticals, known as bioprocess, involves a wide range of techniques. In this review, we discuss the technology involved in the bioprocess and describe the available strategies and main advances in microbial fermentation and purification process to obtain biopharmaceuticals.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biopharmaceuticals; Fermentation process; Biotechnology; Upstream process; Downstream process.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500051
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Choice of sterilizing/disinfecting agent: determination of the Decimal ReductionTime (D-Value) BJPS
Mazzola,Priscila Gava; Jozala,Angela Faustino; Novaes,Letícia Célia de Lencastre; Moriel,Patricia; Penna,Thereza Christina Vessoni.
Efforts to diminish the transmission of infections include programs in which disinfectants play a crucial role. Hospital surfaces and medical devices are potential sources of cross contamination, and each instrument, surface or area in a health care unit can be responsible for spread of infection. The decimal reduction time was used to study and compare the behavior of selected strains of microorganisms. The highest D-values for various bacteria were obtained for the following solutions: (i) 0.1% sodium dichloroisocyanurate (pH 7.0) - E. coli and A. calcoaceticus (D = 5.9 min); (ii) sodium hypochlorite (pH 7.0) at 0.025% for B. stearothermophilus (D = 24 min), E. coli and E. cloacae (D = 7.5 min); at 0.05% for B. stearothermophilus (D = 9.4 min) and E....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Disinfection; Disinfectant agents; Decimal reduction time/determination.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400013
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Determination of decimal reduction time (D-value) of chemical agents used in hospital disinfection BJM
Mazzola,Priscila Gava; Martins,Alzira Maria da Silva; Penna,Thereza Christina Vessoni.
Prior to selecting disinfectant for low, intermediate and high (sterilizing) levels, the decimal reduction time, D-value, for the most common or persistent bacteria identified on a medical device or at a health care facility should be determined. The D-value was determined by inoculating 100 mL of disinfecting solution with 1 mL of a bacterial suspension. At regular intervals, 1 mL aliquots of this mixture were transferred to 8 mL of growth media containing a neutralizing agent, and incubated at optimal conditions for the microorganism. B. stearothermophilus and E. coli were the most resistant bacteria for the disinfecting and sterilizing procedures.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decimal reduction time; Sanitizers; Desinfection.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500011
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Isolation and purification of bromelain from waste peel of pineapple for therapeutic application BABT
Bresolin,Iara Rocha Antunes Pereira; Bresolin,Igor Tadeu Lazzarotto; Silveira,Edgar; Tambourgi,Elias Basile; Mazzola,Priscila Gava.
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromelain; Pineapple peel; Downstream processing; Waste; Therapeutic applications; Enzymatic activity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012
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Microalgae: Cultivation Aspects and Bioactive Compounds BABT
Coêlho,Diego de Freitas; Tundisi,Louise Lacalendola; Cerqueira,Keilla Santos; Rodrigues,Jacqueline Rego da Silva; Mazzola,Priscila Gava; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de.
Abstract Microalgae are aquatic unicellular microorganisms that can be found both in freshwater and marine systems; are capable of photosynthesis; and can grow as individual cells or associated in chains or small colonies. Microalgae cultivation has gained large momentum among researchers in the past decades due to their ability to produce value metabolites, remarkable photosynthetic efficiency, and versatile nature. The wide technological potential, and thus increasing amount of scattered knowledge, may become the very first barrier that a post graduating student, or any non-specialist reader, will face when introduced to the subject. In this review paper, we access the core aspects of microalgae technology, covering their main characteristics, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microalgae; Microalgae Selection and Isolation; Green Products; Microalgae Cultivation; Bioactive Compounds.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000101000
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Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents BJPS
Mazzola,Priscila Gava; Jozala,Angela Faustino; Novaes,Letícia Célia de Lencastre; Moriel,Patricia; Penna,Thereza Christina Vessoni.
Due to the growing number of outbreaks of infection in hospital and nurseries, it becomes essential to set up a sanitation program that indicates that the appropriate chemical agent was chosen for application in the most effective way. Validating the effectiveness of decontamination and disinfection is an important and often challenging task. In order to study and compare the behavior of selected microorganisms, they were submitted to minimal inhibitory concentration (MIC). The MIC intervals, which reduced bacteria populations over 6 log10, were: 59 to 156 mg/L of quaternary ammonium compounds (QACs); 63 to 10000 mg/L of chlorhexidine; 1375 to 3250 mg/L of glutaraldehyde; 39 to 246 mg/L of formaldehyde; 43750 to 87500 mg/L of ethanol; 1250 to 6250 mg/L of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Minimal inhibitory concentration; Disinfectant agents; Sterilizing agents; Sanitation/programs.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200008
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Pharmacovigilance in oncology: pattern of spontaneous notifications, incidence of adverse drug reactions and under-reporting BJPS
Visacri,Marília Berlofa; Souza,Cinthia Madeira de; Pimentel,Rafaela; Barbosa,Cristina Rosa; Sato,Catarina Miyako Shibata; Granja,Silvia; Marialva,Mécia de; Lima,Carmen Silvia Passos; Mazzola,Priscila Gava; Moriel,Patrícia.
The high toxicity and narrow therapeutic window of antineoplastic agents makes pharmacovigilance studies essential in oncology. The objectives of the current study were to analyze the pattern of spontaneous notifications of adverse drug reactions (ADRs) in oncology patients and to analyze the incidence of ADRs reported by outpatients on antineoplastic treatment in a tertiary care teaching hospital. To compose the pattern of ADR, the notification forms of reactions in oncology patients in 2010 were reviewed, and the reactions were classified based on the drug involved, mechanism, causality, and severity. To evaluate the incidence of reactions, a questionnaire at the time of chemotherapy was included, and the severity was classified based on the Common...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pharmacovigilance; Oncovigilance; Drugs/adverse reactions; Adverse drug reactions/under-reporting; Adverse drug reactions/Spontaneous notification; Clinical pharmacy.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200411
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Prevalence of potential drug-drug interactions in the intensive care unit of a Brazilian teaching hospital BJPS
Rodrigues,Aline Teotonio; Stahlschmidt,Rebeca; Granja,Silvia; Pilger,Diogo; Falcão,Antonio Luis Eiras; Mazzola,Priscila Gava.
Abstract Patients in intensive care unit are prescribed large numbers of drugs, highlighting the need to study potential Drug-Drug Interactions in this environment. The aim of this study was to delineate the prevalence and risk of potential drug-drug interactions between medications administered to patients in an ICU. This cross-sectional observational study was conducted during 12 months, in an adult ICU of a teaching hospital. Inclusion criteria were: prescriptions with 2 or more drugs of patients admitted to the ICU for > 24 hours and age of ≥18 years. Potential Drug-Drug Interactions were quantified and classified through MicromedexTM database. The 369 prescriptions included in this study had 205 different drugs, with an average of 13.04 ± 4.26...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Potential drug-drug interactions; Intensive care unit; Patient safety; University hospitals..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100624
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Stability of furosemide and aminophylline in parenteral solutions BJPS
Santos,Carolina Alves dos; Mazzola,Priscila Gava; Polacwiecz,Bronislaw; Knirsch,Marcos Camargo; Cholewa,Olívia; Penna,Thereza Christina Vessoni.
Parenteral solutions (PS) are one of the most commonly used drug delivery vehicles. Interactions among the drug, components in the drug's formulation, and the PS can result in the formation of inactive complexes that limit efficacy or increase side effects. The aim of this work was to evaluate possible interactions between the drugs and PS, assess drug stability and to identify degradation products after 20 h at room temperature. Furosemide (FSM) and Aminophylline (APN) were added to PS containing either 20% mannitol or 0.9% NaCl at pH 6.5-7.5 and 10-11. Their behavior was studied individually and as an admixture, after 1 h oxidation with H2O2, using a spectrophotometer and HPLC. Individually, FSM and APN added to 20% mannitol and 0.9% NaCl solutions had...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parenteral solutions; Spectrophotometer; High Performance Liquid Chromatography; Furosemide; Aminophylline.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100011
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The influence of pH, polyethylene glycol and polyacrylic acid on the stability of stem bromelain BJPS
Novaes,Letícia Celia de Lencastre; Jozala,Angela Faustino; Mazzola,Priscila Gava; Júnior,Adalberto Pessoa.
Enzyme stability is critical in biotechnology, pharmaceutical and cosmetic industries. Investigations on this subject have drawn attention because of its practical application. Bromelain is a thiol-endopeptidase, obtained from pineapple (Ananas comosus), known for its clinical and therapeutic applications, particularly to selective burn debridement and improvement of antibiotic action and anti-inflammatory activities. To date, the use of bromelain in pharmacological or industrial applications is limited, due to commercial availability, costs, and sensitivity to pH and temperature. Therefore, a better understanding of enzyme stability would be of great interest. The aim of this study was to evaluate bromelain activity and stability in several pH (2.0 to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromelain/pH stability; Polyethylene glycol/enzyme stability; Polyacrylic acid/enzyme stability; Enzyme/stability.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200371
Registros recuperados: 10
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