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Registros recuperados: 9
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Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria Inra
Chapuis, M.-P.; Loiseau, A.; Michalakis, Y.; Lecoq, M..
Because of the scarcity of polymorphic genetic markers available in locust species, only a few population genetics studies have been carried out on this taxon. We isolated and characterized 11 polymorphic microsatellite loci in the pest locust Locusta migratoria capito, and described experimental conditions for polymerase chain reaction (PCR) multiplexing and simultaneously genotyping these loci. The number of alleles per locus ranged from six to 25, and the expected heterozygosity ranged from 0.431 to 0.957. Results of cross-taxon amplification tests are reported in six other Locusta migratoria subspecies, six species of the Oedipodinae subfamily and two other pest locust species
Tipo: Journal Article Palavras-chave: CRIQUET MIGRATEUR; POLYMORPHISME; MARQUEUR GENETIQUE LOCUST; LOCUSTA MIGRATORIA; MICROSATELLITES; NULL ALLELES; ORTHOPTERA; PEST.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200769fce925&uri=/notices/prodinra1/2007/08/
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Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria (vol 5, pg 554, 2005) - corrigendum Inra
Chapuis, M.P.; Loiseau, A.; Michalakis, Y.; Lecoq, M.; Estoup, A..
Because of the scarcity of polymorphic genetic markers available in locust species, only a few population genetics studies have been carried out on this taxon. We isolated and characterized 11 polymorphic microsatellite loci in the pest locust Locusta migratoria capito, and described experimental conditions for polymerase chain reaction (PCR) multiplexing and simultaneously genotyping these loci. The number of alleles per locus ranged from six to 25, and the expected heterozygosity ranged from 0.431 to 0.957. Results of cross-taxon amplification tests are reported in six other Locusta migratoria subspecies, six species of the Oedipodinae subfamily and two other pest locust species
Tipo: Journal Article Palavras-chave: CRIQUET MIGRATEUR; POLYMORPHISME; MARQUEUR GENETIQUE LOCUST; LOCUSTA MIGRATORIA; MICROSATELLITES; NULL ALLELES; ORTHOPTERA; PEST.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011bec98eb7&uri=/notices/prodinra1/2011/06/
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Dynamics of the multiplicity of cellular infection in a plant virus Inra
Gutiérrez, S.; Yvon, M.; Thébaud, G.; Monsion, B.; Michalakis, Y.; Blanc, S..
Recombination, complementation and competition profoundly influence virus evolution and epidemiology. Since viruses are intracellular parasites, the basic parameter determining the potential for such interactions is the multiplicity of cellular infection (cellular MOI), i.e. the number of viral genome units that effectively infect a cell. The cellular MOI values that prevail in host organisms have rarely been investigated, and whether they remain constant or change widely during host invasion is totally unknown. Here, we fill this experimental gap by presenting the first detailed analysis of the dynamics of the cellular MOI during colonization of a host plant by a virus. Our results reveal ample variations between different leaf levels during the course of...
Tipo: Journal Article Palavras-chave: CAULIFLOWER MOSAIC VIRUS.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010c4b911fc&uri=/notices/prodinra1/2010/12/
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Genetic variation for parental effects on the propensity to gregarise in Locusta migratoria Inra
Chapuis, M.P.; Estoup, A.; Augé-Sabatier, A.; Foucart, A.; Lecoq, M.; Michalakis, Y..
Background: Environmental parental effects can have important ecological and evolutionary consequences, yet little is known about genetic variation among populations in the plastic responses of offspring phenotypes to parental environmental conditions. This type of variation may lead to rapid phenotypic divergence among populations and facilitate speciation. With respect to density-dependent phenotypic plasticity, locust species (Orthoptera: family Acrididae), exhibit spectacular developmental and behavioural shifts in response to population density, called phase change. Given the significance of phase change in locust outbreaks and control, its triggering processes have been widely investigated. Whereas crowding within the lifetime of both offspring and...
Tipo: Journal Article Palavras-chave: CRIQUET MIGRATEUR.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010c566ffaa&uri=/notices/prodinra1/2010/12/
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Natural multiplicity of infection of plant cells by genomes of Cauliflower Inra
Gutierrez, S.; Yvon, M.; Monsion, B.; Thébaud, G.; Michalakis, Y.; Blanc, S..
The number of viral genomes co-infecting host cells during the course of a systemic infection(the multiplicity of cellular infection, MOI) is a parameter of foremost importance for severalaspects of the biology of viruses. While a low MOI may reduce the intra-cellular competitionbetween genetic variants, it also greatly limits the extent of possible complementation, and de facto reduces the recombination frequency. In contrast, a high MOI would augment thepossibility of trans-complementation between variants, and the recombination frequency, butpotentially also enhance accumulation of defective genomes and increase competition forreplication. These simple examples illustrate the predicted contrasted effects of MOI on viralgenomes and populations, which...
Tipo: Conference Paper Palavras-chave: CAULIFLOWER MOSAIC VIRUS; MULTIPLICITE D'INFECTION .
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010c5ae694a&uri=/notices/prodinra1/2010/05/
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Outbreaks, gene flow and effective population size in the migratory locust Locusta migratoria: a regional scale comparative survey Inra
Chapuis, M.P.; Loiseau, A.; Michalakis, Y.; Lecoq, M.; Franc, A.; Estoup, A..
The potential effect of population outbreaks on within and between genetic variation of populations in pest species has rarely been assessed. In this study, we compare patterns of genetic variation in different sets of historically frequently outbreaking and rarely outbreaking populations of an agricultural pest of major importance, the migratory locust, Locusta migratoria. We analyse genetic variation within and between 24 populations at 14 microsatellites in Western Europe, where only ancient and low-intensity outbreaks have been reported (non-outbreaking populations), and in Madagascar and Northern China, where frequent and intense outbreak events have been recorded over the last century (outbreaking populations). Our comparative survey shows that (i)...
Tipo: Journal Article Palavras-chave: CRIQUET MIGRATEUR; LOCUSTA MIGRATORIA.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010d7360fb0&uri=/notices/prodinra1/2010/12/
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Parental crowding influences life-history traits in Locusta migratoria females Inra
Chapuis, M.P.; Crespin, L.; Estoup, A.; Augé-Sabatier, A.; Foucart, A.; Lecoq, M.; Michalakis, Y..
Parental environments could play an important role in controlling insect outbreaks, provided they influence changes in physiological, developmental or behavioural life-history traits related to fluctuations in population density. However, the potential implication of parental influence in density-related changes in life-history traits remains unclear in many insects that exhibit fluctuating population dynamics, particularly locusts. In this study, we report a laboratory experiment, which enabled us to characterize the life-history trait modifications induced by parental crowding of female individuals from a frequently outbreaking population of Locusta migratoria (Linnaeus) (Orthoptera: Acrididae). We found that a rearing history of crowding led to reduced...
Tipo: Journal Article Palavras-chave: CRIQUET CONTROLLED LABORATORY EXPERIMENT; CROWDING; DENSITY-DEPENDENT POLYPHENISM; LIFE-HISTORY; LOCUST; OFFSPRING QUALITY; PARENTAL EFFECTS; OUTBREAK; PEST; REPRODUCTIVE TIMING.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20102d27fcfe&uri=/notices/prodinra1/2010/12/
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Recombination every day: abundant recombination in a virus during a single multi-cellular host infection Inra
Froissart, R.; Roze, D.; Uzest, M.; Galibert, L.; Blanc, S.; Michalakis, Y..
Viral recombination can dramatically impact evolution and epidemiology. In viruses, the recombination rate depends on the frequency of genetic exchange between different viral genomes within an infected host cell and on the frequency at which such co-infections occur. While the recombination rate has been recently evaluated in experimentally co-infected cell cultures for several viruses, direct quantification at the most biologically significant level, that of a host infection, is still lacking. This study fills this gap using the cauliflower mosaic virus as a model. We distributed four neutral markers along the viral genome, and co-inoculated host plants with marker-containing and wild-type viruses. The frequency of recombinant genomes was evaluated 21 d...
Tipo: Journal Article Palavras-chave: VIRUS RECOMBINANT CAULIFLOWER MOSAIC VIRUS; VIRAL RECOMBINATION; VIRUS POPULATION.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007cdfa7eca&uri=/notices/prodinra1/2007/09/
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The virulence-transmission trade-off in vector-borne plant viruses: a review of (non-)existing studies Inra
Froissart, R.; Doumayrou, J.; Vuillaume, F.; Alizon, S.; Michalakis, Y..
The adaptive hypothesis invoked to explain why parasites harm their hosts is known as the trade-off hypothesis, which states that increased parasite transmission comes at the cost of shorter infection duration. This correlation arises because both transmission and disease-induced mortality (i.e. virulence) are increasing functions of parasite within-host density. There is, however, a glaring lack of empirical data to support this hypothesis. Here, we review empirical investigations reporting to what extent within-host viral accumulation determines the transmission rate and the virulence of vector-borne plant viruses. Studies suggest that the correlation between within-plant viral accumulation and transmission rate of natural isolates is positive....
Tipo: Journal Article Palavras-chave: EVOLUTION; DISEASE; VIRAL ACCUMULATION; VIRULENCE; TRANSMISSION.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011e7058620&uri=/notices/prodinra1/2011/01/
Registros recuperados: 9
Primeira ... 1 ... Última
 

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