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Frequency of 2,3,5-triphenyltetrazolium chloride (TTC) non-reducing bacteria in pasteurized milk Rev. Microbiol.
Beloti,Vanerli; Barros,Márcia A.F.; Freitas,Júlio C. de; Nero,Luís A.; Souza,Juliana A. de; Santana,Elsa H.W.; Franco,Bernadette. D.G.M..
2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for enumeration of microbial colonies in solid culture media, being a key component of the dry rehydratable film system used for microbiological analysis of food. This dye is colorless in the oxidized form and red when reduced by microorganisms, due to formation of formazan. In this study, TTC was added to Plate Count Agar (PCA) for enumeration of microorganisms in thirty four pasteurized milk samples, with the aim to verify the frequency of microorganisms that are unable to reduce TTC. Milk samples were decimally diluted in saline and pour-plated in PCA plus 0.015% TTC. Colonies were counted after 24h and 48 h of incubation at 35oC. From a total of 50,574 colonies, 19,665 (38.88%) did not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2; 3; 5-triphenyltetrazolium chloride TTC pasteurized milk microbial counts.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200009
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