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An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation. Repositório Alice
PAULO, E. M.; VASCONCELOS, M. P.; OLIVEIRA, I. S.; AFFE, H. M. J.; NASCIMENTO, R. dos S.; MELO, I. S. de; ROQUE, M. R. de A.; ASSIS, S. A. de.
Abstract: The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs,...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Triagem; Exopolissacarídeos; Análise de laboratório; Polissacarídeo; Screening; Exopolysaccharides; Lactic acid bacteria.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/963060
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Potential of probiotic bacteria for the control of the postharvest disease of strawberry caused by Botritis cinerea. Repositório Alice
PANSA, C. C.; CASTELIANI, A. G. B.; PAULO, E. M.; SILVA, J. L. da; PARMA, M. M.; FERNANDES, T. A. R.; MELO, I. S. de.
Abstract: Botrytis cinerea is a phytopathogenic fungus known popularly as gray mold, it is transmitted by air and currently it has caused serious damage to strawberry crop, impacting the culture in all development stages. The disease occurs after penetration of the fungus in the plant tissues through wounds, caused during handling or collecting. One of the most common alternatives used to control the pathogen is the use of synthetic fungicides, presenting itself as the causal agent of environmental damage and human health. Aiming to reduce this problem, the search for new strategies that have less impact on the environment has been identified as a priority, currently the biological control is emerging in agricultural systems as one of the most viable...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Probiotic; Botrytis cinerea; Biological control; Probiotics.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1037846
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Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain. Repositório Alice
PAULO, E. M.; BOFFO, E. F.; BRANCO, A.; VALENTE, A. M. M. P.; MELO, I. S. de; FERREIRA, A. G.; ROQUE, M. R. de A.; ASSIS, S. A. de.
ABSTRACT: The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28°C for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Exopolysaccharide; Polymer; Dextran; Bactéria; Leuconostoc pseudomesenteroides; Dextran; Biopolymers.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/942711
Registros recuperados: 3
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