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Fermentation and recovery of L-glutamic acid from cassava starch hydrolysate by ion-exchange resin column Rev. Microbiol.
Nampoothiri,K. Madhavan; Pandey,Ashok.
Investigations were carried out with the aim of producing L-glutamic acid from Brevibacterium sp. by utilizing a locally available starchy substrate, cassava (Manihot esculenta Crantz). Initial studies were carried out in shake flasks, which showed that even though the yield was high with 85-90 DE (Dextrose Equivalent value), the maximum conversion yield (~34%) was obtained by using only partially digested starch hydrolysate, i.e. 45-50 DE. Fermentations were carried out in batch mode in a 5 L fermenter, using suitably diluted cassava starch hydrolysate, using a 85-90 DE value hydrolysate. Media supplemented with nutrients resulted in an accumulation of 21 g/L glutamic acid with a fairly high (66.3%) conversation yield of glucose to glutamic acid (based on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brevibacterium sp.; L-glutamic acid; Cassava hydrolysate; Batch and fed-batch process; Ion-exchange resin; Purification.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300013
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Isolation, identification and physiological study of Lactobacillus fermentum LPB for use as probiotic in chickens BJM
Reque,Elizete de F.; Pandey,Ashok; Franco,Sebastião G.; Soccol,Carlos R..
Studies were carried out to isolate and identify microorganisms for probiotic use for chickens. Selection of strains included various criteria such as agreement with bio-safety aspects, viability during storage, tolerance to low pH/ gastric juice, bile, and antimicrobial activity. The strains were isolated from the crop, proventriculus, gizzard, ileum and caeca of chicken. Decimal dilution of the contents of these segments were mixed with MRS medium and incubated for 48 h at 37°C under anaerobiosis. The identity of the culture was based on characteristics of lactobacilli as presented in the Bergey’s Manual of Determinative Bacteriology, carrying out bacterioscopy (morphology), Gram stain, growth at 15 and 45°C, and fermentation of different carbon sources....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Probiotic; Poultry; Lactobacillus fermentum.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400012
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