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Development of cDNA-derived SSR markers and their efficiency in diversity assessment of Cymbidium accessions Electron. J. Biotechnol.
Moe,Kyaw Thu; Hong,Woo-Ju; Kwon,Soon-Wook; Park,Yong-Jin.
Cymbidium spp. are popular flowering plants. Assessment of the genetic diversity in cultivated Cymbidium facilitates conservation of germplasm and subsequent cultivar improvement. Thus, it is important to develop more efficient polymorphic DNA markers. Although more motifs (403) were identified and more primers (206) were designed in the genomic library compared to the cDNA library, a larger number of successful primers were obtained from the cDNA library (59.9%) than from genomic DNA library (51.1%). However, higher PIC and gene diversity were identified in genomic SSRs. The average allele number per locus was also higher in genomic SSRs (7.3) than EST-SSRs (5.2), among the 24 evaluated Cymbidium accessions. AT/TA was comparatively high in...
Tipo: Journal article Palavras-chave: CDNA library; Cymbidium; Enriched library; Molecular diversity; SSR.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200004
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Evaluation of genetic diversity and linkage disequilibrium in Korean-bred rice varieties using SSR markers Electron. J. Biotechnol.
Wang,Xiao-Qiang; Kwon,Soon-Wook; Park,Yong-Jin.
Background: In order to evaluate the variation among different rice types, the genetic diversity in a rice collection composed by 59 breeding lines, 23 landraces, 18 weedy rice lines, and 35 introduced lines that collected from countries worldwide was analyzed using 134 simple sequence repeat markers. Results: In total, 1264 alleles were identified (average, 9.43 per locus). Rare alleles made up a large portion (58.4%) of the detected alleles, and 29 unique alleles associated with rice accessions were also discovered. A model-based structural analysis revealed the presence of three subpopulations. The genetic relationships revealed by the neighbour-joining tree method were fairly consistent with the structure-based membership assignments for most of the...
Tipo: Journal article Palavras-chave: Landraces; Linkage disequilibrium; Population structure; Rice; Simple sequence repeats.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500011
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Genetic diversity and population structure analysis of strawberry (Fragaria x ananassa Duch.) using SSR markers Electron. J. Biotechnol.
Yoon,Min-Young; Moe,Kyaw Thu; Kim,Dae-Young; Rho,Il-Rae; Kim,Su; Kim,Ki-Taek; Won,Mi-Kyoung; Chung,Jong-Wook; Park,Yong-Jin.
In total, 18 simple sequence repeat (SSR) markers were used to analyze the genetic diversity and population structure of 59 accessions of cultivated strawberry (Fragaria x ananassa Duch.) from Korea, Germany, United States, United Kingdom, and Japan. In total, 101 alleles were detected with an average of 5.6 per locus and 21 specific alleles were identified. Notably, one genotype (Blonoli from Germany) possessed a maximum of 10 different unique alleles specific to each genotype. The gene diversity varied from 0.027 (EMPaEKO1B) to 0.791 (CFACT110), with an average value of 0.509. PIC values ranged from 0.026 to 0.762 (average 0.454). A model-based structure analysis revealed the presence of two populations. The accessions that were clearly assigned to a...
Tipo: Journal article Palavras-chave: Genetic diversity; Population structure; SSR markers; Strawberry.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200006
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