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Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize Electron. J. Biotechnol.
Fontana Capalbo,Deise Maria; Valicente,Fernando Hercule; Oliveira Moraes,Iracema de; Pelizer,Lúcia Helena.
The well-known entomopathogenic bacterium Bacillus thuringiensis (Bt) produces a spore-crystal complex which is responsible for its biocide characteristic, and the bacterium can be obtained by fermentation, either in liquid or semi-solid substrates. This paper presents a successful way to achieve solid-state fermentation of active Bt var. tolworthi (Btt) against Spodoptera frugiperda (fall armyworm) in corn. More than 10(9) CFU/g were obtained using humidified rice as substrate maintained in polypropylene bags. This active complex (substrate plus spore-crystal of Bt) was prepared in order to obtain 2 x 10(6) spores/mL; the final suspension then sprayed via tractor on corn fields. On the treated plants, mortality of neonate larvae was 100% within two days...
Tipo: Journal article
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000200009
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Spirulina platensis growth estimation by pH determination at different cultivations conditions Electron. J. Biotechnol.
Pelizer,Lúcia Helena; Carvalho,J.C.M.; Sato,Sunao; de Oliveira Moraes,Iracema.
Spirulina platensis is a cyanobacterium that has a high protein content and therefore, a high nutritional value. It can be cultivated either in a liquid or in a solid culture. When cultivated in aqueous culture the cell growth can be determined by following the optical density. On the other hand, when produced by solid cultivation the growth can be determined only indirectly, such as, through determination of the protein content of the fermenting solids. In this work the possibility of estimating cell growth by pH determination was verified. From the results it was concluded that pH and protein production (solid or surface culture) or cell content (liquid culture) correlate well, therefore pH determination seems to be a good method to determine cell growth.
Tipo: Journal article
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300009
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Use of volatile fatty acids salts in the production of xanthan gum Electron. J. Biotechnol.
Correa Leite,José Alberto; Pozzi,Eloiza; Pelizer,Lúcia Helena; Zaiat,Marcelo; Barboza,Marlei.
Background: The aim of this study was the production of xanthan gum from salts of volatile fatty acids, which can be generated in anaerobic processes for the production of hydrogen from organic wastewaters. Xanthan gum was produced with three different acid salts used to replace the traditional citrate, which is normally used in the culture for the production of this biopolymer. The volatile fatty acids (VFA) salts used were sodium acetate 0.0328 M, sodium propionate 0.0219 M, and sodium butyrate 0.0164 M. Results: The values of biomass yield, (Yp/x) obtained were 9.2 g/g for acetate, 11.78 g/g for citrate, 11.80 g/g for butyrate and 14.59 g/g for propionate, while the values of the product yield (Yp/s), were 0.92; 0.59; 0.71 and 0.72 for acetate, citrate,...
Tipo: Journal article Palavras-chave: Rheology; Volatile fatty acids; Xanthan gum.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200006
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