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Registros recuperados: 3
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Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands Genet. Mol. Biol.
Franco,Maurício Machaim; Antunes,Robson Carlos; Oliveira,Katiana Mello de; Pereira,Cícero Donizete; Biase,Fernando Henrique; Nunes,Francis de Morais Franco; Goulart,Luiz Ricardo.
Fourty-six non-castrated, halothane-free, male Landrace pigs were genotyped by PCR-RFLP for the Rsa I polymorphism in the PIT1 gene and classified into AA and AB genotypes. Total RNA was extracted from the pituitaries and the relative quantities of growth hormone (GH) mRNA were determined by semi-quantitative RT-PCR. Pigs with the AB genotype had higher levels of GH mRNA than those with the AA genotype (p = 0.034; Kruskal-Wallis test). This result suggests that the Rsa I polymorphism may be involved in Pit-1 protein expression or function, which in turn may influence GH transcription and expression. Thus, the Rsa I PIT1 gene polymorphism in this pig line may be used as a molecular marker to identify higher GH expression and possibly select for carcass and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PIT1 gene; Growth hormone mRNA; Semi-quantitative RT-PCR; Pig.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100003
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Esterase profile in a pyrethroid-resistant Brazilian strain of the cattle tick Boophilus microplus (Acari, Ixodidae) Genet. Mol. Biol.
Baffi,Milla Alves; Pereira,Cícero Donizete; Souza,Guilherme Rocha Lino de; Bonetti,Ana Maria; Ceron,Carlos Roberto; Gourlart,Luiz Ricardo.
The cattle tick Boophilus microplus causes great damage in livestock and is considered one of the most important tropical ectoparasites. The traditional method of control is based on the intensive use of pesticides, however the indiscriminate use of these compounds over the years has led to the selection of resistant ticks. Hydrolases, particularly esterases (EST), have been reported to be associated with acaricide resistance in B. microplus. We compared the esterase profile of susceptible and cypermethrin-resistant strains of adult B. microplus and a pyrethroid susceptible reference strain (the Mozzo strain) using polyacrylamide gel electrophoresis and specific staining. The electrophoretic profiles of protein extracts revealed the presence of four...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Boophilus microplus; Pesticides; Resistance; Esterases.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500016
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Esterase profile in the postembryonic development of Rhipicephalusmicroplus PAB
Baffi,Milla Alves; Pereira,Cícero Donizete; Souza,Guilherme Rocha Lino de; Ceron,Carlos Roberto; Bonetti,Ana Maria.
The objective of this work was to analyze the pattern of esterase activity in the development stages of Rhipicephalus microplus by nondenaturing polyacrylamide gel electrophoresis using specific staining for esterase. The electrophoretical results revealed the presence of nine regions displaying esterase activity, stained with both alpha-naphthyl acetate and beta-naphthyl acetate, and classified as alpha-beta-esterase. Stage-specific esterases were found, with the first nymphal and larval stages showing the greatest esterase activity throughout the development. An esterase called EST-4 was detected only in males and was considered sex-specific. There are differences in the esterase profile among the different postembryonic development stages of R. microplus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle tick; Resistance to acaricides; Tick control.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000800016
Registros recuperados: 3
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