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Genetic diversity analysis of traditional and improved cultivars of Pakistani rice (Oryza sativa L.) using RAPD markers Electron. J. Biotechnol.
Rabbani,Malik Ashiq; Pervaiz,Zahida Hassan; Masood,Muhammad Shahid.
The molecular marker is a useful tool for assessing genetic variations and resolving cultivar identities. Information on genetic diversity and relationships among rice genotypes from Pakistan is currently very limited. The objective of this study was to evaluate the genetic polymorphisms and identities of 10 traditional, 28 improved and 2 Japanese cultivars of rice using the random amplified polymorphic DNA technique. Twenty-five decamer-primers could generate a total of 208 RAPD fragments, of which 186 or 89.4% were polymorphic. The number of amplification products produced by each primer varied from 4 to 16 with an average of 8.3 bands primer-1. The size of amplified fragments were ranged from 200 to 4000 bp. Pair-wise Nei and Li's similarity had...
Tipo: Journal article Palavras-chave: Cultivar discrimination; Genetic variability; Oryza sativa; Pakistani rice; RAPDs.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300006
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Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces Electron. J. Biotechnol.
Pervaiz,Zahida Hassan; Rabbani,M. Ashiq; Khaliq,Ishtiaq; Pearce,Stephen R; Malik,Salman A.
Genetic diversity underlies the improvement of crops by plant breeding. Land races of rice (Oryza sativa L.) can contain some valuable alleles not common in modern germplasm. The aim here was to measure genetic diversity and its effect on agronomic traits among rice land-race genotypes grown in Pakistan. Diversity was measured using thirty-five microsatellite markers and seventy-five genotypes. Among the markers used a total of 142 alleles were detected at 32 polymorphic SSR loci, while three loci were monomorphic in Pakistani rice landraces. The number of alleles identified by each marker ranged from 2 to 13 with a mean of 4.4. Size differences between the smallest and largest alleles varied from 11bp to 71bp. Polymorphism information content ranged from...
Tipo: Journal article Palavras-chave: Genetic diversity; Landraces; Microsatellite markers; Oryza sativa L.; Pakistan; Rice.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300004
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