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Registros recuperados: 19
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Aroma compounds in eleven edible mushroom species: relationship between volatile profile and sensorial characteristics IPB - Escola Superior Agrária
Pinho, P. Guedes de; Ribeiro, Bárbara; Gonçalves, Rui; Baptista, Paula; Valentão, P.; Seabra, R.M.; Andrade, P.B..
Volatile and semi-volatile components of 11 wild edible mushrooms, Suillus bellini, Suillus luteus, Suillus granulatus, Tricholomopsis rutilans, Hygrophorus agathosmus, Amanita rubescens, Russula cyanoxantha, Boletus edulis, Tricholoma equestre, Fistulina hepatica and Cantharellus cibarius, were determined by headspace solidphase microextraction and by liquid extraction combined with gas chromatographymass spectrometry.
Tipo: ConferenceObject Palavras-chave: Volatile and semi-volatile components; Wild edible mushrooms.
Ano: 2008 URL: http://hdl.handle.net/10198/5934
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Aroma compounds in eleven edible mushroom species: relationship between volatile profile and sensorial characteristics IPB - Escola Superior Agrária
Pinho, P. Guedes de; Ribeiro, Bárbara; Gonçalves, Rui; Baptista, Paula; Valentão, P.; Seabra, R.M.; Andrade, P.B..
Tipo: ConferenceObject Palavras-chave: Wild edible mushrooms; Volatile compounds; HS - SPME; GC-MS.
Ano: 2007 URL: http://hdl.handle.net/10198/5756
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Aroma compounds in eleven edible mushroom species: relationship between volatile profile and sensorial characteristics IPB - Escola Superior Agrária
Pinho, P. Guedes de; Ribeiro, Bárbara; Gonçalves, Rui; Baptista, Paula; Valentão, P.; Seabra, R.M.; Andrade, P.B..
Tipo: ConferenceObject Palavras-chave: Wild edible mushrooms; Aroma compounds; GC- MSMS; Sensorial characteristics.
Ano: 2008 URL: http://hdl.handle.net/10198/5735
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Chemical assessment and in vitro antioxidant capacity of Ficus carica latex IPB - Escola Superior Agrária
Oliveira, A.P.; Silva, L.R.; Ferreres, F.; Pinho, P. Guedes de; Valentão, P.; Silva, B.M.; Pereira, J.A.; Andrade, P.B..
Ficus species possess latex-like material within their vasculatures, affording protection and selfhealing from physical attacks. In this work, metabolite profiling was performed on Ficus carica latex. Volatiles profile was determined by HS-SPME/GC-IT-MS, with 34 compounds being identified, distributed by distinct chemical classes: 5 aldehydes, 7 alcohols, 1 ketone, 9 monoterpenes, 9 sesquiterpenes and 3 other compounds. Sesquiterpenes constituted the most abundant class in latex (ca. 91% of total identified compounds). Organic acids composition was also characterized, by HPLC-UV, and oxalic, citric, malic, quinic, shikimic and fumaric acids were determined. Malic and shikimic acids were present in higher amounts (ca. 26%, each). The antioxidant potential...
Tipo: Article Palavras-chave: Ficus carica latex; Volatiles; Organic acids; Antioxidant potential; Acetylcholinesterase inhibition.
Ano: 2010 URL: http://hdl.handle.net/10198/3116
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Correlation between the pattern volatiles and the overall aroma of wild edible mushrooms IPB - Escola Superior Agrária
Pinho, P. Guedes de; Ribeiro, Bárbara; Gonçalves, Rui; Baptista, Paula; Valentão, P.; Seabra, R.M.; Andrade, P.B..
Volatile and semivolatile components of 11 wild edible mushrooms, Suillus bellini, Suillus luteus, Suillus granulatus, Tricholomopsis rutilans, Hygrophorus agathosmus, Amanita rubescens, Russula cyanoxantha, Boletus edulis, Tricholoma equestre, Fistulina hepatica, and Cantharellus cibarius, were determined by headspace solid-phase microextraction (HS-SPME) and by liquid extraction combined with gas chromatography–mass spectrometry (GC-MS). Fifty volatiles and nonvolatiles components were formally identified and 13 others were tentatively identified. Using sensorial analysis, the descriptors “mushroomlike”, “farm-feed”, “floral”, “honeylike”, “hay-herb”, and “nutty” were obtained. A correlation between sensory descriptors and volatiles was observed by...
Tipo: Article Palavras-chave: Wild edible mushrooms; Semivolatile compounds; Sensorial analysis; Volatile; GC-MS; Solid-phase microextraction.
Ano: 2008 URL: http://hdl.handle.net/10198/4405
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Determination of low molecular weight volatiles in Ficus carica using HS-SPME and GC/FID IPB - Escola Superior Agrária
Oliveira, A.P.; Silva, L.R.; Andrade, P.B.; Valentão, P.; Silva, B.M.; Pereira, J.A.; Pinho, P. Guedes de.
Ficus carica L. is one of the earliest cultivated fruit trees, having an important consumption in Mediterranean countries. In this work, the volatile compound profiles of two characteristic Portuguese white varieties (‘‘Pingo de Mel” and ‘‘Branca Tradicional”) was determined by HS-SPME and GC/FID. Leaves, pulps and peels, submitted to freezing and lyophilisation treatments, were analysed. The two varieties presented a similar profile composed of eight volatile compounds: acetaldehyde, ethyl acetate, methanol, ethanol, hexanal, limonene, (E)-2-hexenal and octanal. The total volatile content was different among the vegetal materials, following the order leaves > peels > pulps. Methanol and ethanol are the major compounds in all samples. The developed...
Tipo: Article Palavras-chave: Ficus carica; Volatile compounds; HS-SPME; GC/FID.
Ano: 2010 URL: http://hdl.handle.net/10198/3115
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Evolution of Brassica rapa L. var. rapa volatile composition by HS-SPME and GC/IT-MS. IPB - Escola Superior Agrária
Taveira, M.; Fernandes, F.; Pinho, P. Guedes de; Andrade, P.B.; Pereira, J.A.; Valentão, P..
Brassica rapa var. rapa L. (turnip) is highly appreciated and consumed by human. In this work, the volatile profile of B. rapa var. rapa was studied during the maturation process. The volatiles of seeds, sprouts with 6 and 9 days, and adult plant were determined using headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/ion trap-mass spectrometry (GC/IT-MS). Several constituents, including alcohols, aldehydes, esters, ketones, norisoprenoids, nitrogen and sulphur compounds were characterized, totalizing 64 compounds. Isothiocyanates are the main volatiles in all matrices, being 3-butenyl isothiocyanate the major compound. Qualitative and quantitative differences were found among the analysed materials. Nitrogen and sulphur...
Tipo: Article Palavras-chave: Brassica rapa var. rapa L.; Turnip; Sprouts; Seeds; Volatile compounds.
Ano: 2009 URL: http://hdl.handle.net/10198/3110
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Fatty acid composition of wild edible mushrooms species: a comparative study IPB - Escola Superior Agrária
Ribeiro, Bárbara; Pinho, P. Guedes de; Andrade, P.B.; Baptista, Paula; Valentão, P..
A comparative study was developed on the total fatty acids composition of twelve wild edible mushroom species (Suillus bellini, Suillus luteus, Suillus granulatus, Hygrophorus agathosmus, Amanita rubescens, Russula cyanoxantha, Boletus edulis, Tricholoma equestre, Fistulina hepatica, Cantharellus cibarius, Amanita caesarea and Hydnum rufescens). In order to define qualitative and quantitative profiles, combined fatty acids were hydrolyzed with potassium hydroxide/methanol and all free compounds were derived to their methyl ester forms with methanolic boron trifluoride, followed by analysis by GC-MS. Thirty fatty acids were determined. As far as we know, the fatty acid profiles of A. caesarea and H. rufescens are described for the first time. As for the...
Tipo: Article Palavras-chave: Wild edible mushrooms; Fatty acids; Comparative analysis.
Ano: 2009 URL: http://hdl.handle.net/10198/4409
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Further insights into the latex metabolite profile of Ficus carica IPB - Escola Superior Agrária
Oliveira, A.P.; Silva, L.R.; Andrade, P.B.; Valentão, P.; Silva, B.M.; Gonçalves, Rui; Pereira, J.A.; Pinho, P. Guedes de.
Latex is a sticky emulsion that exudes upon damage from specialized canals from several plants. It contains several biologically active compounds, such as phytosterols, fatty acids, and amino acids. In plants, these compounds are involved in the interaction between plants, insects, and the environment. Despite its chemical, biological, and ecological importance, Ficus carica latex is still poorly studied. To improve the knowledge on the metabolite profile of this matrix, a targeted metabolite analysis was performed in a representative sample from F. carica latex. Seven phytosterols were determined by gas chromatography-ion trap mass spectrometry (GC-ITMS) and high-performance liquid chromatography coupled to diode array detection (HPLC-DAD), with...
Tipo: Article Palavras-chave: Ficus carica latex; Phytosterols; Free and combined fatty acids; Free amino acids.
Ano: 2010 URL: http://hdl.handle.net/10198/4561
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Headspace solid-phase microextraction and gás chromatography/ion trap-mass spectrometry applied to a living system: Pieris brassicae fed with kale IPB - Escola Superior Agrária
Fernandes, F.; Pereira, D.M.; Pinho, P. Guedes de; Valentão, P.; Pereira, J.A.; Bento, Albino; Andrade, P.B..
The influence of Pieris brassicae feeding on kale was monitored, by evaluating its effect on the volatiles released by the plant through time. This is the first study applying headspace solid-phase microextraction (HS-SPME) and gas chromatography/ion trap-mass spectrometry to an isolated insect, as most studies analyse the insect–plant system as a whole, being unable to evaluate the contribution of the insect itself. Substantial differences were noticed between the volatiles composition of kale before and after the insect’s attack. More than 60 compounds were found, including terpenes, lipoxygenase pathway by-products, ketones, norisoprenoids, etc. After insect attack, l-camphor, sabinene and a-thujene were found and limonene and eucalyptol suffered a...
Tipo: Article Palavras-chave: Brassica oleracea L. var. acephala; Kale; Pieris brassicae; Volatile compounds; HS-SPME; GC/IT-MS.
Ano: 2009 URL: http://hdl.handle.net/10198/3112
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Interaction between Hypholoma fasciculare and the ectomycorrhizal fungus Pisolithus tinctorius IPB - Escola Superior Agrária
Baptista, Paula; Pinho, P. Guedes de; Malheiro, R.; Tavares, Rui Manuel; Lino-Neto, Teresa.
In the past few years, cord-forming basidiomycetes have received considerable attention in their growth strategies in the presence of other fungi (saprotrophic and pathogenic). In the present study, the in vitro interaction between the cord-forming basidiomycete Hypholoma fasciculare and the ectomycorrhizal fungus Pisolithus tinctorius was studied. Dual-cultures were established and the radial fungal growth, hyphae morphology and production of volatile compounds were evaluated during interaction. Growth of P. tinctorius is severely inhibited by H. fasciculare even before mycelia contact, suggesting a mechanism of antagonism at a distance. Following contact with H. fasciculare mycelia, P. tinctorius displayed distinctive hyphal interference with collapsed...
Tipo: ConferenceObject Palavras-chave: Hypholoma fasciculare; Pisolithus tinctorius; Volatile compounds; Fungal interaction.
Ano: 2010 URL: http://hdl.handle.net/10198/4493
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Metabolic fate of dietary volatile compounds in Pieris brassicae IPB - Escola Superior Agrária
Fernandes, F.; Pereira, D.M.; Pinho, P. Guedes de; Valentão, P.; Pereira, J.A.; Bento, Albino; Andrade, P.B..
In this work, the evolution of the qualitative and quantitative profile of the volatile fraction of Pieris brassicae after feeding on Brassica oleracea var. acephala (kale) was monitored through time. HS-SPME/GC-MS was applied to both the host plant and the living insect and its excrements. A total of seventy seven compounds (lipoxygenase pathway by-products, nitrogen compounds, norisoprenoids, sulphur compounds, terpenes, among others) were identified. Thirty eight compounds were identified in insect after 2 h of starvation and forty eight compounds in excrements. Qualitative and quantitative changes were detected along time. Dimethyldisulfide, dimethyltrisulfide, limonene and eugenol were major compounds for all analysed times in both matrices, being...
Tipo: Article Palavras-chave: Pieris brassicae; Excrements; Metabolization; Volatile compounds; HS-SPME; GC/IT–MS.
Ano: 2009 URL: http://hdl.handle.net/10198/3108
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Metabolite profiling of wild edible mushrooms species IPB - Escola Superior Agrária
Valentão, P.; Ribeiro, Bárbara; Pinho, P. Guedes de; Baptista, Paula; Andrade, P.B..
Mushrooms are of increasing importance in modern nutrition and medicine. Trás-os-Montes region (north-eastern Portugal) is known for the variety of its soils and diversity of climate conditions. This variability assumes an important role in mushroom production, explaining why this region is recognized as one of the richest ones in wild edible species. A metabolomics approach was applied to twelve wild edible mushroom species (Suillus bellini, Suillus luteus, Suillus granulatus, Hygrophorus agathosmus, Amanita rubescens, Russula cyanoxantha, Boletus edulis, Tricholoma equestre, Fistulina hepatica, Cantharellus cibarius, Amanita caesarea and Hydnum rufescens) collected in this area, with the purposes of improving the knowledge about their chemical profiles...
Tipo: ConferenceObject Palavras-chave: Wild edible mushrooms; Metabolomics.
Ano: 2010 URL: http://hdl.handle.net/10198/4494
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Phytochemical profiles and inhibitory effect on free radical-induced human erythrocyte damage of Dracaena draco leaf: A potential novel antioxidant agent IPB - Escola Superior Agrária
Santos, R.P.; Mendes, L.S.; Silva, B.M.; Pinho, P. Guedes de; Valentão, P.; Andrade, P.B.; Pereira, J.A.; Carvalho, M..
The present study reports for the first time the metabolite profile and antioxidant activity of aqueous extract obtained from Dracaena draco L. leaf. Volatiles profile was determined by HS-SPME/GC-IT-MS, with 34 compounds being identified, distributed by distinct chemical classes: 2 alcohols, 5 aldehydes, 16 carotenoid derivatives and 8 terpenic compounds. Carotenoid derivative compounds constituted the most abundant class in leaf (representing 45% of total identified compounds). Phenolics profile was determined by HPLC/DAD and 9 constituents were identified: 2 hydroxycinnamic acid derivatives – 5- O-caffeoylquinic and 3,5-O-dicaffeoylquinic acids; 4 hydroxycinnamic acids – caffeic, p-coumaric, ferulic and sinapic acids and 3 flavonol glycosides –...
Tipo: Article Palavras-chave: Dracaena draco leaf; Volatiles; Polyphenols; Organic acids; Haemolysis inhibition; Antioxidant activity.
Ano: 2011 URL: http://hdl.handle.net/10198/3124
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Systemic release of volatiles by Brassica oleracea var. acephala induced by Pieris brassicae predation. IPB - Escola Superior Agrária
Fernandes, F.; Pereira, D.M.; Pinho, P. Guedes de; Valentão, P.; Oliveira, I.; Pereira, J.A.; Andrade, P.B..
Pieris brassicae is a specialist on crucifers and its interactions with some of its host plants have been increasingly studied given their importance in pest management, chemical ecology and entomology. Plants are known to respond to insect attack by releasing volatiles that can either warn neighbour plants to the presence of a predator or attract insect parasitoids, thus reducing the attack efficiency. The influence of P. brassicae feeding on Brassica oleracea L. var. acephala (kale) was investigated, namely, its effect in the volatiles released by the plant through time. Substantial differences, mainly quantitative, were found between the volatiles composition of B. oleracea var. acephala before the attack and after insect feeding. More than 40 compounds...
Tipo: ConferenceObject
Ano: 2009 URL: http://hdl.handle.net/10198/4840
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Volatile composition of three species of wild mushrooms from Trás-os-Montes region using HS-SPME and GC/IT-MS IPB - Escola Superior Agrária
Nogueira, Liliana; Malheiro, R.; Pinho, P. Guedes de; Baptista, Paula.
Trás-os-Montes region is considered one of the richest regions of Portugal concerning the existence of wild mushrooms. In the present work, was studied the volatile profile of three species of wild mushrooms, Hebeloma crustuliniforme, Clitocybe odora and Tricholoma fracticum. These species have different flavours, namely radish-, anise-like odor, and not distinctive or slightly mealy odor, respectively. The volatile fractions were determined in fresh mushrooms by headspace solid-phase microextraction (HS-SPME) combined with gas-chromatography/ion trap-mass spectrometry (GC/IT-MS). Several volatile compounds were identified, belonging to different chemical classes. The three studied mushroom species reported different volatile profiles as presented in...
Tipo: ConferenceObject Palavras-chave: Volatile profile; Wild mushrooms; GC/IT-MS.
Ano: 2010 URL: http://hdl.handle.net/10198/4590
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Volatile constituents throughout Brassica oleracea L. var. acephala germination IPB - Escola Superior Agrária
Fernandes, F.; Pinho, P. Guedes de; Valentão, P.; Pereira, J.A.; Andrade, P.B..
In this work, the volatile composition of kale (Brassica oleracea L. var. acephala) and its variation during germination were monitored during the first 9 days of seedling development by headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/ion trap-mass spectrometry (GC/IT-MS). Differences were found among the materials in the distinct analyzed periods. A total of 66 volatile compounds, distributed in several chemical classes, were determined: alcohols, carbonyl compounds (ketones, aldehydes, and esters), norisoprenoids, and terpenes, among others, sulfur compounds being the most abundant group in seeds and sprouts that exhibited allyl isothiocyanate as the major compound. Leaves of fully developed ground plant had the highest...
Tipo: Article Palavras-chave: Brassica oleracea L. var. acephala; Kale; Leaves; Sprouts; Seeds; Volatile compounds.
Ano: 2009 URL: http://hdl.handle.net/10198/3118
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Volatile constituents throughout Brassica oleracea L. var. acephala germination. IPB - Escola Superior Agrária
Fernandes, F.; Gomes, D.; Pinho, P. Guedes de; Valentão, P.; Pereira, J.A.; Andrade, P.B..
Brassicaceae family are commonly grown and consumed worldwide. Belonging to this group, kale (Brassica oleraceIJe var. acephala) is important in traditional farming systems in the Iberian Peninsula. While its seeds have been used for human consumption as oil, or mixed with some food products, sprouts, the germination form, are favoured for their nutritional value [1].
Tipo: ConferenceObject
Ano: 2009 URL: http://hdl.handle.net/10198/5139
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Volatile profiling of Ficus carica varieties by HS-SPME and GC-IT-MS. IPB - Escola Superior Agrária
Oliveira, A.P.; Silva, L.R.; Pinho, P. Guedes de; Gil Izquierdo, A.; Valentão, P.; Silva, B.M.; Pereira, J.A.; Andrade, P.B..
Aroma is one of the essential parameters for the evaluation of fruit quality, with volatile components being determinant for this characteristic. During this work, the volatile profile of fresh fruits (pulp and peel) and leaves of Portuguese Ficus carica L. white (‘‘Pingo de Mel” and ‘‘Branca Tradicional”) and dark (‘‘Borrasota Tradicional”, ‘‘Verbera Preta” and ‘‘Preta Tradicional”) varieties were characterised by HSSPME/ GC–IT-MS. Fifty-nine compounds were identified and distributed by distinct chemical classes (aldehydes, alcohols, ketones, esters, monoterpenes, sesquiterpenes, norisoprenoids), with 39 being reported for the first time in this species. Principal component analysis of semi-quantitative data showed that pulps and peels are distinguished...
Tipo: Article Palavras-chave: Ficus carica; Volatile compounds; HS-SPME; GC–IT-MS; PCA.
Ano: 2010 URL: http://hdl.handle.net/10198/3120
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