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Application of library-independent microbial source tracking methods for identifying the sources of faecal contamination in coastal areas ArchiMer
Gourmelon, Michele; Caprais, Marie-paule; Le Mennec, Cecile; Mieszkin, Sophie; Ponthoreau, C.; Gendronneau, M..
Faecal contamination sources were identified in coastal areas around the Guerande-Atlantique peninsula using two microbial source tracking (MST) methods: (i) Bacteroidales host-specific 16S rRNA gene markers measured by real-time PCR and (ii) F-specific bacteriophage (FRNAPH) genotyping. Both methods were used on 63 water samples from 7 water courses. HF183 marker and bacteriophage genogroup II (FRNAPH II) were detected in all water samples and in the majority of water samples, respectively, from La Torre stream (W5), Piriac (W2), R2000 (W3) and Mazy (W7) rain water drains, and also detected, less frequently, in Le Nau drain (W4), suggesting contamination by human faecal sources at these sites. These human markers were weakly detected in Pouliguen channel...
Tipo: Text Palavras-chave: Bacteroidales; F-specific RNA bacteriophages; Faecal bacterial indicators; Microbial source tracking; Water.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00003/11384/10304.pdf
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Suivi des coques du Croisic en 1998. Bilan synthétique. ArchiMer
Fleury, Pierre-gildas; Langlade, Aime; Le Coguic, Marie-jo; Thebault, Anne; Chellet, B.; Ponthoreau, C.; Devulder, G..
Suite aux mortalités massives de coques apparues au Croisic en août 1997, des études ont été mises en place en 1998, avec une coordination des travaux des différents laboratoires impliqués: suivi de la température et de la matière organique dans le sédiment, suivi des animaux et observation des parasites. Les principaux résultats sont: - l'enregistrement de brusques amplitudes thermiques dans le sédiment; - des taux anormalement élevés de matière organique dans le sédiment (20 à 40%) à l'automne 1997, puis des taux plus habituels (<2%) après les tempêtes de l'hiver 1997-98 ; - un affaiblissement important de la vitalité musculaire des animaux en juillet; - une infestation relativement faible en trématodes virulents (Labratrema minimus), mais dont les...
Tipo: Text Palavras-chave: Coques; Cerastoderma edule; Le Croisic; Sédiment; Elevage; Mortalité; Croissance; Vitalité; Maturation; Pathologie; Cockles; Cerastoderma edule; Le Croisic (France); Sediment; Rearing; Mortallty; Growlh; Vitality; Maturation; Pathology.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00107/21848/19435.pdf
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