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Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence’ - doi: 10.4025/actasciagron.v34i2.12257 Agronomy
Prizão, Eliane Cristina; Universidade Estadual de Maringá; Gonçalves, Letícia de Menezes; Universidade Estadual de Maringá; Gutierre, Maria Auxiliadora; Universidade Estadual de Maringá; Mangolin, Claudete Aparecida; Universidade Estadual de Maringá; Machado, Maria de Fátima Pires da Silva; Universidade Estadual de Maringá.
No previous studies have investigated the use of graphite instead of activated charcoal in orchids. In this work, different concentrations of activated charcoal or graphite were added to KC medium to darken the culture medium and stimulate the in vitro propagation of Cattleya bicolor and of a double hybrid orchid (‘BLC Pastoral Innocence’). The seedlings were inoculated on growth regulator-free KC medium; the effects of activated charcoal (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) and graphite (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) concentrations were tested separately. Each flask contained fifteen seedlings, and the experiments had a randomised, two-way factorial design. The two tested variables were culture medium and illumination levels; eleven culture...
Tipo: Laboratory analysis Palavras-chave: Activated charcoal; Graphite; Orchids; Micropropagation.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257
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Use of complex supplements and light-differential effects for micropropagation of Hadrolaelia purpurata (= Laelia purpurata) and Encyclia randii orchids - doi: 10.4025/actasciagron.v34i4.12333 Agronomy
Gonçalves, Letícia Menezes; Universidade Estadual de Maringá; Prizão, Eliane Cristina; Universidade Estadual de Maringá; Gutierre, Maria Auxiliadora Milaneze; Universidade Estadual de Maringá; Mangolin, Claudete Aparecida; Universidade Estadual de Maringá; Machado, Maria de Fátima Pires da Silva; Universidade Estadual de Maringá.
In the current study, complex (coconut water and ripe-banana pulp) and specific (micronutrients, peptone and activated charcoal) supplements were added to Knudson-C medium (KC) medium to stimulate the in vitro propagation of Hadrolaelia purpurata (=Laelia purpurata) and Encyclia randii orchid species. The highest number of H. purpurata seedlings occurred in KC medium containing 90 g L-1 banana pulp maintained with 24-hour illumination. Positive effects of the banana pulp addition in KC medium were reported for the formation of seedlings and leaves from E. randii seeds. The seedling number from seeds of E. randii maintained with continuous illumination was also high in KC medium containing coconut water. Positive effects due to the addition of peptone were...
Tipo: Laboratory analysis Palavras-chave: Genetica vegetal Biologia Celular e Genética asymbiotic seed germination; Banana coconut water; Peptone; Micronutrients; Orchidaceae genetica vegatal.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12333
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