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Registros recuperados: 4
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An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation. Repositório Alice
PAULO, E. M.; VASCONCELOS, M. P.; OLIVEIRA, I. S.; AFFE, H. M. J.; NASCIMENTO, R. dos S.; MELO, I. S. de; ROQUE, M. R. de A.; ASSIS, S. A. de.
Abstract: The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs,...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Triagem; Exopolissacarídeos; Análise de laboratório; Polissacarídeo; Screening; Exopolysaccharides; Lactic acid bacteria.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/963060
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Análise colorimétrica de polissacarídeos do solo como resposta ao manejo agrícola. Repositório Alice
FRIGHETTO, R. T. S.; VALARINI, P. J.; ROQUE, M. R. de A.; FELIPE, A.; TOKESHI, H..
1997
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Análise colorimétrica; Polissacarídeos; Manejo agrícola.
Ano: 1997 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1057545
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Antifungal activity of bacterial strains from the rhizosphere of Stachytarpheta crassifolia. Repositório Alice
SANTOS, S. N.; OLIVEIRA, L. K. X.; MELO, I. S. de; VELOSO, E. da S.; ROQUE, M. R. de A..
This study evaluated the antifungal action of biomolecules produced from the secondary metabolism of bacterial strains found in the rhizosphere of semi arid plants against human pathogenic Candida albicans. Crude extracts were obtained using ethyl acetate as an organic solvent and the bioactivity was assessed with a bioautography technique. The results showed that bacterial strains, Alcaligenes faecalis MRbS12 and Bacillus cereus MRbS26, had compounds with antifungal bioactivity. The largest inhibition zones for both compounds were located on spots with Rf values below 0.500, indicating that the molecules possibly had polar characteristics. These results suggested that microorganisms found in the environment could have bioprospecting potential.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biomolecules; Bioautoghaphy; Antifungal activity; Alcaligenes faecalis; Bacillus cereus; Candida albicans; Bactéria; Rizosfera; Controle biológico; Rhizobiales; Antifungal agents; Biological control.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/898255
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Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain. Repositório Alice
PAULO, E. M.; BOFFO, E. F.; BRANCO, A.; VALENTE, A. M. M. P.; MELO, I. S. de; FERREIRA, A. G.; ROQUE, M. R. de A.; ASSIS, S. A. de.
ABSTRACT: The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28°C for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Exopolysaccharide; Polymer; Dextran; Bactéria; Leuconostoc pseudomesenteroides; Dextran; Biopolymers.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/942711
Registros recuperados: 4
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