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Registros recuperados: 4
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1H nuclear magnetic resonance spectroscopy-based studies of the metabolism of food-borne carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline by human intestinal microbiota Inra
Humblot, C.; Combourieu, B.; Väisänen, M.J.; Furet, J.P.; Delort, A.M.; Rabot, S..
2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) is a mutagenic/carcinogenic compound formed from meat and fish during cooking. Following ingestion, IQ is metabolized mainly by liver xenobiotic-metabolizing enzymes, but intestinal bacteria may also contribute to its biotransformation. The aim of this study was to investigate the metabolism of IQ by the human intestinal microbiota. Following incubation of IQ (200 microM) under anoxic conditions with 100-fold dilutions of stools freshly collected from three healthy volunteers, we quantified residual IQ by high-pressure liquid chromatography (HPLC) analysis and characterized the production of IQ metabolites by in situ (1)H nuclear magnetic resonance ((1)H-NMR) spectroscopic analysis of crude incubation media. In...
Tipo: Journal Article Palavras-chave: INTESTINAL MICROBIOTA; HUMAN; RAT; CARCINOGEN; MICROFLORE INTESTINALE.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008cc87ce43&uri=/notices/prodinra1/2008/06/
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A new type of bacterial sulfatase reveals a novel maturation pathway in prokaryotes Inra
Berteau, O.; Guillot, A.; Benjdia, A.; Rabot, S..
Sulfatases are a highly conserved family of enzymes found in all three domains of life. To be active, sulfatases undergo a unique post-translational modification leading to the conversion of either a critical cysteine ("Cys-type" sulfatases) or a serine ("Ser-type" sulfatases) into a Calpha-formylglycine (FGly). This conversion depends on a strictly conserved sequence called "sulfatase signature" (C/S)XPXR. In a search for new enzymes from the human microbiota, we identified the first sulfatase from Firmicutes. Matrix-assisted laser desorption ionization time-of-flight analysis revealed that this enzyme undergoes conversion of its critical cysteine residue into FGly, even though it has a modified (C/S)XAXR sulfatase signature. Examination of the bacterial...
Tipo: Journal Article Palavras-chave: AMINO-ACID CONVERSION; SULFATASE; HUMAN MICROBIOTA; AMINO-ACID MOTIF; MOLECULAR SEQUENCE DATA; SULFATASES MATURATION.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20086b5166a4&uri=/notices/prodinra1/2010/11/
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Constitutive delivery of bovine beta-lactoglobulin to the digestive tracts of gnotobiotic mice by engineered Lactobacillus casei Inra
Hazebrouck, S.; Oozeer, R.; Adel-Patient, K.; Langella, P.; Rabot, S.; Wal, J.M.; Corthier, G..
The gut microbiota is critical for maturation of the immune system. Recent evidence suggests that early establishment of lactobacilli in the intestinal microbiota, during neonatal colonization or by probiotic supplementation, could prevent the development of allergic disorders. Postnatal maturation of the gut immune system with allergen-producing lactobacilli colonizing the digestive tract could then affect the development of further allergic sensitization. In this paper, we describe construction of a recombinant Lactobacillus casei strain that can constitutively deliver bovine beta-lactoglobulin (BLG), a major cow's milk allergen, to the guts of gnotobiotic mice. The blg gene was inserted into the L. casei chromosome downstream of an endogenous promoter....
Tipo: Journal Article Palavras-chave: BOVINE BETA-LACTOGLOBULIN; ALLERGEN; PROBIOTIC; INTESTINAL MICROBIOTA; GUT IMMUNE SYSTEM; DIGESTIVE TRACT.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008e9e33f45&uri=/notices/prodinra1/2008/06/
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Survival and metabolic activity of selected strains of Propionibacterium freudenreichii in the gastrointestinal tract of human microbiota-associated rats Inra
Lan, A.; Bruneau, A.; Philippe, C.; Rochet, V.; Rouault, A.; Hervé, C.; Roland, N.; Rabot, S.; Jan, G..
In addition to their use in cheese technology, dairy propionibacteria have been identified as potential probiotics. However, to have a probiotic effect, propionibacteria have to survive and to remain metabolically active in the digestive tract. The aim of the present study was to investigate the survival and metabolic activity of Propionibacterium freudenreichii within the gastrointestinal tract of human microbiota-associated rats, and its influence on intestinal microbiota composition and metabolism. Twenty-five dairy Propionibacterium strains were screened for their tolerance towards digestive stresses and their ability to produce propionate in a medium mimicking the content of the human colon. Three strains were selected and a daily dose of 2 £ 1010...
Tipo: Journal Article Palavras-chave: PROPIONIBACTERIUM; FATTY ACIDS; GASTROINTESTINAL TRACT; METABOLIC ACTIVITY.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008ca232066&uri=/notices/prodinra1/2008/04/
Registros recuperados: 4
Primeira ... 1 ... Última
 

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