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Assessment of genetic diversity in Indian ash gourd (Benincasa hispida) accessions using RAPD markers National Institute of Agronomic Research
Pandey, S.; Kumar, S.; Rai, M.; Mishra, U.; Singh, M..
Thirty four (9 cultivars and 25 germplasm) genotypes of ash gourd (Benincasa hispida) have been studied to assess the molecular diversity using RAPD markers. A total of 25 RAPD markers produced 163 amplicons, of which 73 % were found polymorphic indicating a high degree of diversity. RAPD based dendrogram showed dissimilarity values from 0.64 to 0.943 also suggesting that the accessions represent a genetically diverse population. The accession IVAG-223 was clearly separated from rest of the accessions based on the RAPD primers. The dendogram revealed that the accessions from northeastern regions of India are different from the accessions of other parts of India, as they cluster together. On the basis of clustering, it was indicated that cultivars had a...
Tipo: Conference Paper Palavras-chave: Genetic resources; Genetic diversity; Dendrogram; PCR amplification; Germplasm; Molecular diversity.
Ano: 2008 URL: http://hdl.handle.net/2174/193
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Cucurbit research in India: a retrospect National Institute of Agronomic Research
Rai, M.; Pandey, S.; Kumar, S..
Cucurbits belong to family Cucurbitaceae, includes about 118 genera and 825 species. In India, a number of major and minor cucurbits are cultivated, which share about 5.6 % of the total vegetable production. They are consumed in various forms i.e., salad (cucumber, gherkins, long melon), sweet (ash gourd, pointed gourd), pickles (gherkins), and deserts (melons). In India, several research institutes and universities have utilized a number of cultivated and wild species to develop improved varieties and parental lines. About 112 open pollinated varieties of several cucurbits have been recommended for cultivation at national and state levels. Among these, 48 improved varieties in 8 major cucurbits have been identified and recommended through All India...
Tipo: Poster Palavras-chave: Cucurbitaceae; Cucurbitaceous vegetables; Germplasm; Hybrid; Production; Genetic resources; Biotic resistance.
Ano: 2008 URL: http://hdl.handle.net/2174/223
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Sex expression-associated RAPD markers in pointed gourd (Trichosanthes dioica) National Institute of Agronomic Research
Kumar, S.; Singh, B.D.; Sinha, D.P.; Rai, M..
DNA samples were extracted separately from 10 male and 10 female sexual progeny of pointed gourd (Trichosanthes dioica), and 3 plants from a parthenocarpic clone (IIVRPG-105); the DNAs were bulked by sex type. Forty-one random decamer primers were screened with the three bulks in order to identify markers associated with sex expression. A total of 509 amplification products were obtained of which six were associated with sex expression. These six markers were then tested with individual plant DNA samples, and three sex-associated RAPD markers were identified. A 1000 bp amplification product from the primer OPC05 was found to be present in all the male and absent from all the female and parthenocarpic plants. In contrast, a 400 bp amplification product from...
Tipo: Poster Palavras-chave: Trichosanthes dioica; RAPD; Sex expression; Sex-associated; Parthenocarpy.
Ano: 2008 URL: http://hdl.handle.net/2174/262
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