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Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus BJM
Flatschart,Roberto B.; Resende,Maurício.
Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aujeszky’s disease virus; Polymerase chain reaction; Dot-blot hybridization.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013
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Seroconversion and seroreactivity patterns of dairy goats naturally exposed to caprine arthritis-encephalitis virus in Brazil Ciência Rural
Castro,Roberto Soares; Leite,Rômulo Cerqueira; Azevedo,Edisio Oliveira de; Resende,Maurício; Gouveia,Aurora Maria Guimarães.
A labelled avidin-biotin ELISA (lab-ELISA) using repeated serum samples of goats showed a progressive seroconversion with higher seroconversion rate at the period going from the beginning of the breeding up to the last half of lactation (35.0%), compared to that recorded at the beginning of breeding (17.8%)(p<0.05). Furthermore, the seroreactivity pattern, evaluated by a lab-ELISA standard-curve with serum samples collected at 30-40 days intervals during 12 months, was caracterized by high individual variability. No seroreversion was observed and there were higher titers in the group of animals which delivered kids and established a lactation period (n=6; mean titre=913.4 units) compared to the group of goats that failed to conceived (n=4; mean...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goat; Caprine artrhitis-encephalitis virus; Diagnostics; Enzyme immunoassays; Immunity.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000400009
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