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Registros recuperados: 54
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A polyphasic approach for black Aspergillus identification using MALDI-TOF MS. IPB - Escola Superior Agrária
Rodrigues, Paula; Soares, Célia; Santos, Cledir; Fraga, Marcelo; Venâncio, Armando; Lima, Nelson.
The Aspergillus section Nigri is among the best studied fungi, having different commercial applications, but also causing biodeterioration of commodities and food spoilage. Although being well studied, their identification is not straightforward, and, recently, new species have been described in this section1,2,3. These new species were not only separated from their relatives in the section by morphological distinction but also from molecular point of view. The concept of species is clearly abstract and delimitations are very difficult, and often not consensual. Recently, Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) has been used to generate spectrum of protein masses in a range of 2000 to 20000 Da that is a...
Tipo: ConferenceObject Palavras-chave: Black aspergilli; Identification.
Ano: 2009 URL: http://hdl.handle.net/10198/3202
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A polyphasic approach to the identification of aflatoxigenic and non-aflatoxigenic strains of Aspergillus section flavi isolated from portuguese almonds IPB - Escola Superior Agrária
Rodrigues, Paula; Venâncio, Armando; Kozakiewicz, Zofia; Lima, Nelson.
A polyphasic approach consisting of morphological, chemical and molecular characterization was applied to 31 isolates of Aspergillus Section Flavi originating from Portuguese almonds, with the aim of characterizing and identifying aflatoxigenic and non-aflatoxigenic strains. On the basis of morphological characters (mainly colony color on Czapek-Dox agar and conidia morphology), we found two distinct groups among the population under study: 18 isolates (58%) had dark-green colonies and rough conidia, and were classified as A. parasiticus; the remaining 13 isolates (42%) had yellow-green colonies and smooth to finely rough globose conidia, and were classified as A. flavus. Chemical characterization involved the screening of the isolates for aflatoxins B...
Tipo: Article Palavras-chave: Aspergillus; Section flavi; Aflatoxins; Cyclopiazonic acid.
Ano: 2009 URL: http://hdl.handle.net/10198/1052
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Calmodulin gene as good voucher as MALDI-TOF ICMS to identify Portuguese isolates of Aspergillus section Flavi IPB - Escola Superior Agrária
Rodrigues, Paula; Santos, Cledir; Venâncio, Armando; Lima, Nelson.
Tipo: ConferenceObject
Ano: 2010 URL: http://hdl.handle.net/10198/3658
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Calmodulin gene as good voucher as MALDI-TOF ICMS to identify Portuguese isolates of Aspergillus section Flavi IPB - Escola Superior Agrária
Rodrigues, Paula; Santos, Cledir; Venâncio, Armando; Lima, Nelson.
Aspergillus is a large genus, with a complex and ever evolving taxonomy. Section Flavi is one of the most significant Sections in the Genus. Taxonomy and species identification is subject of great interest for scientists aiming to clarify the species concept and limits within the section. Furthermore, this Section comprises both toxigenic and non-toxigenic species/strains, with great interest to biotechnology and food industry. Various genes, namely the rRNA (internal transcribed spacer (ITS) and partial LSU-rRNA regions), calmodulin and ~-tubulin genes, have been widely reported as good markers for Aspergillus species identification, because they are rapid and cost-effective.
Tipo: ConferenceObject Palavras-chave: Fungal identification; DNA sequencing; Mass spectrometry.
Ano: 2010 URL: http://hdl.handle.net/10198/4276
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Characterisation of mycoflora and aflatoxigenic fungi from portuguese almonds: from production to commercialization. IPB - Escola Superior Agrária
Rodrigues, Paula; Venâncio, Armando; Lima, Nelson.
Aflatoxins are among the most carcinogenic natural compounds known, and have been frequently detected in numerous agricultural commodities, nut fruits being among the most contaminated ones. These toxins are a result of the secondary metabolism of fungi belonging to the genus Aspergillus Section Flavi, namelly Aspergillus flavus, A. parasiticus and A. nomius. The early detection of aflatoxigenic fungal contamination and the preventive control of aflatoxin production seems to be the only viable way to respond to the rigorous limits imposed by EU legislation. In Portugal, knowledge on mycoflora and mycotoxigenic profiles of almonds is scarce. Our objective was to characterise portuguese almonds in respect to mycoflora, with strong emphasis on aflatoxigenic...
Tipo: ConferenceObject Palavras-chave: Mycobiota; Mycotoxins.
Ano: 2009 URL: http://hdl.handle.net/10198/4279
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Characterisation of mycoflora and aflatoxigenic fungi from portuguese almonds: from production to commercialization. IPB - Escola Superior Agrária
Rodrigues, Paula; Venâncio, Armando; Lima, Nelson.
Aflatoxins are among the most carcinogenic natural compounds known, and have been frequently detected in numerous agricultural commodities, nut fruits being among the most contaminated ones. These toxins are a result of the secondary metabolism of fungi belonging to the genus Aspergillus Section Flavi, namelly Aspergillus flavus, A. parasiticus and A. nomius. The early detection of aflatoxigenic fungal contamination and the preventive control of aflatoxin production seems to be the only viable way to respond to the rigorous limits imposed by EU legislation. In Portugal, knowledge on mycoflora and mycotoxigenic profiles of almonds is scarce. Our objective was to characterise portuguese almonds in respect to mycoflora, with strong emphasis on aflatoxigenic...
Tipo: ConferenceObject Palavras-chave: Aspergillus; Almonds; Aflatoxins.
Ano: 2009 URL: http://hdl.handle.net/10198/3217
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Characterization and differentiation of aflatoxigenic species of Aspergillus section Flavi by MALDI-TOF MS IPB - Escola Superior Agrária
Rodrigues, Paula; Kallow, W.; Erhard, M.; Welker, M.; Kozakiewickz, Zofia; Lima, Nelson; Venâncio, Armando.
Aspergillus is a large genus, with a complex taxonomy. The genus is easily identifiedd by its characteristic conidiophore, but species identification and differentiation is complex, mainly because it is traditionally based on a range of morphological features. Aspergillus subgenus Circumdati section Flavi, also refered to as the A.dflavus group, has attracted worldwide attention for its industrial use and toxigenic potential. Sectiond Flavi is divided in two groups of species. One includes the aflatoxigenic species A. flavus, A. parasiticus and A. nomius, which cause serious problems in agricultural commodities, and the other one includes the non-aflatoxigenic species A. oryzae, A. sojae and A.dtamarii, traditionally used for production of fermented foods....
Tipo: ConferenceObject Palavras-chave: Aflatoxigenic fungi; Mass spectrometry; Aspergillus flavus; Aspergillus parasiticus.
Ano: 2007 URL: http://hdl.handle.net/10198/5759
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Characterization and identification of aspergillus section flavi isolates from portuguese almonds using a polyphasic approach including MALDI-TOF ICMS IPB - Escola Superior Agrária
Rodrigues, Paula; Santos, Cledir; Venâncio, Armando; Lima, Nelson.
Aspergillus is a large genus, with a complex and ever evolving taxonomy. Section Flavi is one of the most significant sections in the genus, and is one of the best studied among fungi, for the numerous industrial applications as well as for food safety issues. Section Flavi is composed of a large number of very closely related species. While these species are difficult to differentiate morphologically and even genetically, they differ in a characteristic that is of paramount importance for food safety, as some are responsible for the production of the highly toxigenic aflatoxins. Taxonomy and species identification are therefore subject of great interest for scientists aiming to clarify the species concept and limits within the section. In this sense, the...
Tipo: ConferenceObject Palavras-chave: Fungal identification; Mycology; Taxonomy.
Ano: 2010 URL: http://hdl.handle.net/10198/4674
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Characterization of Aspergillus flavus isolated from winemaking grapes IPB - Escola Superior Agrária
Soares, C.M.; Rodrigues, Paula; Kozakiewicz, Zofia; Lima, Nelson; Venâncio, Armando.
Concern about filamentous fungi in vineyards has been traditionally linked to the spoilage of grapes. However, the recent detection of ochratoxin A in wines has increased the interest in mycotoxins. Although not very common in grapes, Aspergillus flavus is occasionally isolated from grapes. A. flavus is the main producer of the well known carcinogenic mycotoxins, the aflatoxins, and the presence of this fungus and aflatoxins is of major food safety concern. The identification of A. flavus is not straightforward due mainly to similarities with its closely related species (A. parasiticus, A. nomius). Various morphological and biochemical parameters may be used for identification: Color of colonies on Czapek Medium (CZ), seriation of conidial heads, conidia...
Tipo: Other Palavras-chave: Aspergillus flavus; Identification.
Ano: 2007 URL: http://hdl.handle.net/10198/954
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Contributions of Micoteca da Universidade do Minho to food mycology IPB - Escola Superior Agrária
Rodrigues, Paula; Soares, Célia; Freitas-Silva, Otniel; Santos, Cledir; Paterson, Russell; Kozakiewicz, Zofia; Venâncio, Armando; Lima, Nelson.
Micoteca da Universidade do Minho (MUM) is a collection of fungi established in 1996. Research involving secondary metabolites in the form of mycotoxins is paramount. An objective is to provide authentic strains for research in biotechnology, life sciences and challenge testing. Knowledge, information and training in mycology are promulgated by the biological resource centre. MUM is involved in projects which address the health and quality risks from fungal contamination of grape products (e.g. wine), apples, cheese, chilies, nuts and corn: Fungi in drinking water is of particular concern. Aspergillus ochraceous, Aspergillus flavus, Penicillium expansum and Fusarium graminearum especially are relevant to our work: Associated mycotoxins are ochratoxin A,...
Tipo: ConferenceObject Palavras-chave: Fungi; Mycotoxins; Fungal collection; Fungal identification.
Ano: 2010 URL: http://hdl.handle.net/10198/4550
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Diversidade de macrofungos comestíveis e não comestíveis num povoamento misto de Pseudotsuga sp., Pinus sp. e Quercus sp. IPB - Escola Superior Agrária
Baptista, Paula; Sousa, Maria João; Rodrigues, Paula; Fernandes, Mariana; Martins, Anabela; Rodrigues, Ana Paula; Dias, Rui; Borges, António.
Tipo: ConferenceObject Palavras-chave: Pseudotsuga sp.; Pinus sp.; Quercus sp.; Macrofungos; Diversidade.
Ano: 2005 URL: http://hdl.handle.net/10198/5745
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Diversidade de macrofungos num povoamento misto de Pseudotsuga sp., Pinus sp. e Quercus sp. IPB - Escola Superior Agrária
Baptista, Paula; Dias, Rui; Sousa, Maria João; Rodrigues, Paula; Martins, Anabela.
Tipo: ConferenceObject Palavras-chave: Macrofungos; Diversidade; Pseudotsuga sp.; Pinus sp.; Quercus sp..
Ano: 2006 URL: http://hdl.handle.net/10198/5752
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Estudo da comunidade macrofúngica associada a souto (Castanea sativa), pinhal (Pinus pinaster) e carvalhal (Quercus pyrenaica), no Nordeste Transmontano IPB - Escola Superior Agrária
Baptista, Paula; Rodrigues, Paula; Sousa, Maria João; Fernandes, Mariana; Martins, Anabela; Rodrigues, Ana Paula; Dias, Rui; Borges, António.
No Nordeste Transmontano existem sistemas florestais e agro-florestais de grande importância sócio-económica, como o castanheiro (Castanea sativa), o pinheiro (Pinus pinaster) e o carvalho negral (Quercus pyrenaica), que estabelecem associações simbióticas com fungos do solo, resultando a formação de ectomicorrizas. A maioria destes fungos, produz estruturas reprodutoras macroscópicas, os carpóforos, esporóforos ou macrofungos, alguns dos quais com elevada valorização comercial. O trabalho que se apresenta desenvolve-se no âmbito de um Projecto AGRO 689 “Demonstração do papel dos macrofungos na vertente agronómica, económica e ambiental no Nordeste Transmontano. Aplicação à produção de plantas de castanheiro, pinheiro e carvalho”, no qual se pretende...
Tipo: Article Palavras-chave: Diversidade macrofúngica; Pinus pinaster; Quercus pyrenaica; Castanea sativa.
Ano: 2005 URL: http://hdl.handle.net/10198/5744
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Estudo da comunidade macrofúngica associada a souto (Castanea sativa), pinhal (Pinus pinaster) e carvalhal (Quercus pyrenaica), no Nordeste Transmontano IPB - Escola Superior Agrária
Baptista, Paula; Rodrigues, Paula; Sousa, Maria João; Fernandes, Mariana; Martins, Anabela; Rodrigues, Ana Paula; Dias, Rui; Borges, António.
Tipo: ConferenceObject
Ano: 2005 URL: http://hdl.handle.net/10198/5743
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Estudos e perspectivas futuras da micorrização in vitro e ex vitro de Castanea sativa Mill. IPB - Escola Superior Agrária
Martins, Anabela; Baptista, Paula; Rodrigues, Paula; Pais, Maria Salomé.
O castanheiro, Castanea sativa Mill. é uma espécie agro-florestal de grande importância económica em Portugal, quer pela produção de fruto quer de madeira, sendo a sua cultura conhecida desde o tempo dos Romanos. A maioria das espécies vegetais possui micorrizas, associações mutualistas altamente evoluídas entre fungos do solo e raízes de plantas. As simbioses acompanharam a evolução da biosfera, sendo um dos casos de cooperação entre genomas de organismos diferentes. As micorrizas constituem as infecções fúngicas mais frequentes: todas as Gimnospérmicas, 83% das dicotiledóneas e 79% das monocotiledóneas são micorrízicas. Através da simbiose, a planta hospedeira recebe nutrientes minerais e o fungo obtém compostos de carbono, contribuindo de forma...
Tipo: ConferenceObject Palavras-chave: Castanea sativa; Pisolithus tinctorius; Micorrização in vitro; Micorrização ex vitro.
Ano: 2005 URL: http://hdl.handle.net/10198/5731
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Estudos e perspectivas futuras das micorrizas e da indução de micorrização in vitro e ex vitro de Castanea sativa Mill. IPB - Escola Superior Agrária
Martins, Anabela; Baptista, Paula; Rodrigues, Paula; Pais, Maria Salomé.
Apresenta-se uma breve retrospectiva dos estudos de micorrização in vitro e ex vitro de plantas de castanheiro micropropagadas e germinantes e os efeitos fisiológicos e químicos da micorrização. Pretende-se ainda perspectivar os estudos em vias de desenvolvimento no domínio da micorrização, dos mecanismos e sinais de associação raiz-fungo e dos efeitos comparativos com associações patogénicas do castanheiro. Estudos de biodiversidade de macrofungos associados ao castanheiro e à aplicação de fungos nativos em programas de micorrização controlada serão também apresentados.
Tipo: Article Palavras-chave: Castanea sativa Mill.; Castanheiro; Pisolithus tinctorius; Micorrização; Micropropagação; Inoculação.
Ano: 2005 URL: http://hdl.handle.net/10198/3884
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HPLC method for simultaneous detection of aflatoxins and cyclopiazonic acid IPB - Escola Superior Agrária
Soares, Célia; Rodrigues, Paula; Freitas-Silva, Otniel; Abrunhosa, Luís; Venâncio, Armando.
Aspergillus species in section Flavi are among the most relevant mycotoxigenic fungi. The organisms are well-known producers of the highly carcinogenic aflatoxins and of other mycotoxins, such as cyclopiazonic acid. Aflatoxins and cyclopiazonic acid analyses can be routinely used for identification purposes within the section. Two separate chromatographic runs with distinct columns and detectors for each toxin were required in previous reports. A straightforward high performance liquid chromatography (HPLC) procedure for the simultaneous detection of these compounds in fungal cultures was developed in the present work using a methanol/water mobile phase, post-column photochemical derivatisation and fluorescence detection. The proposed method was tested...
Tipo: Article Palavras-chave: Aspergillus section Flavi Aspergillus section Flavi; Dual mycotoxin detection Fungal extracts.
Ano: 2010 URL: http://hdl.handle.net/10198/3624
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Identificação de fungos aflatoxigénicos em produtos alimentares. IPB - Escola Superior Agrária
Rodrigues, Paula; Venâncio, Armando; Lima, Nelson.
A contaminação dos alimentos com aflatoxinas e um problema com interesse crescente no que respeita à saúde do consumidor. As aflatoxinas, das quais se destaca a aflatoxina 81 , são micotoxinas de elevado grau toxigénico. Apresentam distribuição mundial e contaminam uma grande variedade de produtos agrícolas, principalmente cereais e frutos de casca rija. As aflatoxinas são produzidas por algumas espécies de Aspergillus secção Flavi. Esta secção integra um elevado numero de espécies muito próximas, tanto ao nível morfológico como molecular, mas que diferem numa característica de elevado interesse para a segurança alimentar a sua capacidade para produzir aflatoxinas. Até ao momento, vários métodos moleculares foram testados para a diferenciação de fungos...
Tipo: Lecture
Ano: 2011 URL: http://hdl.handle.net/10198/4282
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Identification and characterization of Aspergillus flavus and aflatoxins IPB - Escola Superior Agrária
Rodrigues, Paula; Soares, Célia; Kozakiewicz, Zofia; Paterson, Russell; Lima, Nelson; Venâncio, Armando.
Aspergillus flavus is the main producer of the well known carcinogenic aflatoxins. The presence of this fungus and aflatoxins is of huge concern in terms of food safety. The identification of A. flavus is not straightforward due to similarities with closely related species (e.g. A. parasiticus and A. nomius). Also, from the biochemical point of view the closely-related species are able to produce different mycotoxins. In order to clarify the differentiation between species the identification schemes is revisited. Selective media, data from mycotoxins production and molecular biology tools are discussed in order to clarify the concept of A. flavus species.
Tipo: BookPart Palavras-chave: Aspergillus flavus; Aflatoxins; Cyclopiazonic acid.
Ano: 2007 URL: http://hdl.handle.net/10198/933
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Identification of aflatoxigenic and non-aflatoxigenic strains of Aspergillus Section Flavi isolated from Portuguese almonds IPB - Escola Superior Agrária
Rodrigues, Paula; Venâncio, Armando; Kozakiewicz, Zofia; Lima, Nelson.
Aspergillus subgenus Circumdati section Flavi, also referred to as the A. flavus group, has attracted worldwide attention for its industrial use and toxigenic potential. Section Flavi is divided in two groups of species. One includes the aflatoxigenic species A. flavus, A. parasiticus and A. nomius, which cause serious problems in agricultural commodities, and the other one includes the non‐aflatoxigenic species A. oryzae, A. sojae and A. tamarii, traditionally used for production of fermented foods. Differentiating aflatoxigenic from non‐aflatoxigenic species and strains in food commodities is of major importance in food quality control. A polyphasic approach consisting of morphological, chemical and molecular characterization was applied to 31 isolates...
Tipo: ConferenceObject Palavras-chave: Food safety; Aflatoxins; Mycobiota; Nuts.
Ano: 2009 URL: http://hdl.handle.net/10198/4606
Registros recuperados: 54
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