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Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease Chilean J. Agric. Res.
Araya,Carolina; Peña,Elizabeth; Salazar,Erika; Román,Lisset; Medina,Claudia; Mora,Roxana; Aljaro,Agustín; Rosales,Inés-Marlene.
Big-vein disease (BVD) is a widespread and economically damaging disease in lettuce (Lactuca sativa L.). Typical symptoms are chlorotic clearing around leaf veins, leaf deformations, and impaired head development. In this research, we studied the relationship between symptom intensity and protein and viral RNA accumulation in infected plants. Naturally infected lettuce plants, from the field or greenhouse, were classified according to their symptomatology: mild, moderate, severe, and symptomless. Coat protein accumulation was evaluated by a double antibody sandwich/enzyme-linked immunosorbent assay (DAS-ELISA), and RNA levels were studied by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Virus coat...
Tipo: Journal article Palavras-chave: Lettuce big-vein disease; Symptom expression.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100008
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