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An ELISA technique for rapid diagnosis of vibriosis in sea bass Dicentrarchus labrax ArchiMer
Romestand, B; Dragesco, A; Breuil, Gilles; Coste, F; Bouix, G.
Three batches of rabbits were immunized against 2 strains of Vibrio anguillarum (Va V 62 and Va V 408), which cause vibriosis in sea bass Dicentrarchus labrax (L. 1758) Serranidae. Homologous and heterologous reactions were obtained against these Vibrio strains and other bacteria using an improved indirect ELISA technique. Antibody and antigen titrations revealed high immunogenecity (high antibody titers for the antisera) of the 2 Vibrio strains. They correspond to serotype I and show close affinity. The specificity and sensitivity of 1 selected antiserum were tested on brain extracts from healthy sea bass and those infected artificially with V. anguillarum serotypes I and III. Preliminary results confirm the reliability of the technique and warrant its...
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Ano: 1993 URL: http://archimer.ifremer.fr/doc/00193/30400/28992.pdf
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Influence d'un parasite haemarophagen: Lernalocera bronchialis (L., 1767) (Crustacea, Copepoda, (Pennellidae), sur les constances erythrocytaires de son hote Merluccius merluccius (L., 1758) ArchiMer
Bastide-guillaume, C; Douellou, L; Romestand, B; Trilles, J.
A hematological study of erythrocytic constants of a teleost fish, the European hake Merluccius merluccius has been undertaken to evaluate the effect of infestation by the branchial parasitic copepode: Lernaeocera branchialis . As the levels of parasitic infestation increased: a normocytic, normochromic anaemia when fish had one adult female parasite present, a microcytic anaemia when between 2 and 4 adult females were found and a macrocytic anaemia when at least 5 adult females were present. There was no significant difference in body condition or the coefficient of spleen to body weight between the control sample and samples of individuals infested by Lernaeocera branchialis.
Tipo: Text Palavras-chave: Merluccius merluccius; Lernaeocera branchialis; Anemia; Hematology; Parasitic diseases.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/publication-1775.pdf
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Les parasites d'Arnoglossus laterna (Walbaum, 1792), Bothidae, dans le golfe du Lion (cote francaise de la Mediterranee) et leur influence sur les formules leucocytaires des poissons hotes ArchiMer
Douellou, L; Bastide-guillaume, C; Romestand, B; Trilles, J.
Arnoglossus laterna is only infested by intestinal parasites. Each parasite distribution is independent of the distribution of other parasites and also independent of the sex of host. When a fish is infested by a great number of the two helminths Acanthocephaloides and Bothriocephalus clavibothrium , the proportion of lymphocytes tends to decrease and the proportion of neutrophils tends to increase (inflammatory response). Important infestation by Scolex pleuronectis induces an increase of the thrombocyte count for 100 counted leucocytes. After infestation by the nematode Thynnascaris sp. alone or with another parasite, even by small number, the proportion of lymphocytes decreases and the proportion of neutrophils increases.
Tipo: Text Palavras-chave: Arnoglossus laterna; Leukocytes; Hematology; Parasitic diseases; Parasitism.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/publication-1767.pdf
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