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Short-term storage in vitro and large-scale propagation of grapevine genotypes. Repositório Alice
SILVA, R. de C; LUIS, Z. G.; PEREIRA, J. E. S..
The objective of this work was to evaluate the large?scale propagation of grapevine genotypes after short?term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short?term storage, the shoots were propagated in up to five successive subcultures, to assess the large?scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short?term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Ex situ conservation; In vitro conservation; Minimal growth; Vitis.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/930351
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