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A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses Biol. Res.
MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN.
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the...
Tipo: Journal article Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010
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Arabidopsis thaliana: A model host plant to study plant-pathogen interaction using Chilean field isolates of Botrytis cinerea Biol. Res.
GONZÁLEZ,JUAN; REYES,FRANCISCA; SALAS,CARLOS; SANTIAG,MARGARITA; CODRIANSKY,YAEL; COLIHEUQUE,NELSON; SILVA,HERMAN.
One of the fungal pathogens that causes more agriculture damage is Botrytis cinerea. Botrytis is a constant threat to crops because the fungus infects a wide range of host species, both native and cultivated. Furthermore, Botrytis persists on plant debris in and on the soil. Some of the most serious diseases caused by Botrytis include gray mold on vegetables and fruits, such as grapes and strawberries. Botrytis also causes secondary soft rot of fruits and vegetables during storage, transit and at the market. In many plant-pathogen interactions, resistance often is associated with the deposition of callose, accumulation of autofluorescent compounds, the synthesis and accumulation of salicylic acid as well as pathogenesis-related proteins. Arabidopsis...
Tipo: Journal article Palavras-chave: Arabidopsis thaliana; Botrytis cinerea; Plant-pathogen interaction; PR genes.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200004
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Estudio de parámetros hídricos foliares en trigo (Triticum aestivum L.) y su uso en selección de genotipos resistentes a sequía RChHN
ORTIZ,MAURICIO; SILVA,HERMAN; SILVA,PAOLA; ACEVEDO,EDMUNDO.
Los parámetros hídricos foliares ys (potencial de solutos), CRA (contenido relativo de agua) y AO (ajuste osmótico) permiten caracterizar la respuesta de las plantas frente al estrés hídrico e identificar aquellos genotipos mejor adaptados. Sin embargo, estos parámetros presentan una gran influencia del medioambiente lo que dificulta su análisis. En este trabajo se planteó la hipótesis de que es posible caracterizar y seleccionar genotipos resistentes a sequía en base a la evolución de los parámetros hídricos foliares en campo. Se cultivaron 31 genotipos de trigo (Triticum aestivum L.) en dos ensayos de campo, uno regado y otro no regado que recibió sólo 218,3 mm de lluvia invernal. El diseño fue de bloques al azar con dos repeticiones. Entre los 77 y 121...
Tipo: Journal article Palavras-chave: Trigo; Parámetros hídricos foliares; Rendimiento.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2003000200008
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The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana Biol. Res.
URBINA,DANIELA C; SILVA,HERMAN; MEISEL,LEE A.
Thapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering root gravitropism in Arabidopsis thaliana. Thapsigargin concentrations as low as 0.1 µM alter root gravitropism whereas under similar conditions cyclopiazonic acid does not. Furthermore, a fluorescently conjugated thapsigargin (BODIPY FL thapsigargin) suggests that target sites for thapsigargin are located in intracellular organelles in the root distal elongation zone and the root cap, regions known to regulate root gravitropism
Tipo: Journal article Palavras-chave: Arabidopsis thaliana; Ca2+-ATPases; Calcium; Gravitropism; Thapsigargin; Root.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200011
Registros recuperados: 4
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