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Registros recuperados: 5
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Detection of Anaplasma bovis DNA in the Peripheral Blood of Domestic Dogs in Japan OAK
Sakamoto, Leo; Ichikawa, Yasuaki; Sakata, Yoshimi; Matsumoto, Kotaro; Inokuma, Hisashi; 松本, 高太郎; 猪熊, 壽.
The prevalence of Ehrlichia and Anaplasma in 1,427 dogs from 32 Japanese prefectures was evaluated by PCR and DNA nucleotide sequencing. PCR screening demonstrated that 18 dogs (1.3%) were positive for Anaplasmataceae. Sequence analysis revealed that 14 of the amplicons were most closely related to Wolbachia spp., symbionts of Dirofilaria immitis, whereas three were identified as Anaplasma bovis. The remaining amplicon could not be sequenced. Almost the entire sequence of 16S rRNA (1,452 bp) from one of the positive specimens was determined, and subsequent phylogenetic analysis confirmed that the detected sequence was that of A. bovis. This is the first detection of A. bovis DNA fragments in dogs. Species-specific nested PCR showed that 15 (1.1%) of the...
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3083
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Detection of Borrelia garinii, Borrelia tanukii and Borrelia sp. closely related to Borrelia valaisiana in Ixodes ticks removed from dogs and cats in Japan. OAK
Hiraoka, Hiroko; Shimada, Yojiro; Sakata, Yoshimi; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Masuzawa, Toshiyuki; Inokuma, Hisashi.
Ticks removed from 1136 dogs and 134 cats all over Japan were examined for Borrelia infection by PCR and sequencing. The 5S-23S rDNA intergenic spacer of Borrelia was detected from two Ixodes persulcatus ticks from two dogs and two unidentified Ixodes spp. from another two dogs in Hokkaido, and two Ixodes granulatus ticks from two cats in Okinawa. Additional 2 I. granulatus from the same cats also showed positive. Sequence analysis of the PCR products revealed that the one from Hokkaido was similar to B. garinii, the three from Hokkaido to B. tanukii, and the four from Okinawa to a novel Borrelia sp. closely related to B. valaisiana. The data was confirmed by analysis of the flagellin gene sequence. Infected ticks carried by companion animals can be...
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/743
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Detection of DNA of 'Candidatus Mycoplasma haemominutum' and Spiroplasma sp. in unfed ticks collected from vegetation in Japan OAK
Taroura, Shoko; Shimada, Yojiro; Sakata, Yoshimi; Miyama, Takako; Hiraoka, Hiroko; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽.
DNA fragments of 'Candidatus Mycoplasma haemominutum', a feline heamobartonella pathogen, were detected from unfed Ixodes ovatus collected from vegetation in Hokkaido, Fukushima and Yamaguchi Prefectures, and unfed Haemaphysalis flava in Yamaguchi Prefecture. This finding suggests that ixodid tick is a possible vector of 'C. Mycoplasma haemominutum'. Spiroplasma DNA was also detected from unfed I. ovatus in Hokkaido, Fukushima and Yamaguchi Prefectures. The analysis of nucleotides sequence suggested that this Spiroplasma was distinct from registered species.
Palavras-chave: Candidatus Mycoplasma haemominutum; Spiroplasma sp; Tick.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1654
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Detection of Rickettsial DNA in ixodid ticks recovered from dogs and cats in Japan OAK
Hiraoka, Hiroko; Shimada, Yojiro; Sakata, Yoshimi; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽.
DNA from ticks recovered from 1137 dogs and 133 cats from Ell over Japan were examined for Rickettsia infection by citrate synthase gene (gltA)-based PCR and partial nucleotide sequencing. A total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested PCR. Sequence analysis was successfully performed on 102 samples. DNA of Rickettsia japonica or closely related Rickettsia spp. strains were detected from 38 licks in 16 prefectures mainly in western Japan. The other 33, detected from 13 prefectures including Hokkaido and Okinawa, were found to be Rickettsia helvetica or closely related strains. A total of 29 DNA that showed highest homology with Rickettsia akari or closely related strains were detected in 19...
Palavras-chave: Canine; Epidemiology; Feline; Rickettsia; Tick.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/923
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Epidemiological survey of Babesia gibsoni infection in dogs in eastern Japan OAK
Miyama, Takako; Sakata, Yoshimi; Shimada, Yojiro; Ogino, Shoji; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; Nagasawa, Hideyuki; Inokuma, Hisashi.
To determine the distribution of Babesia gibsoni infection in dogs in the eastern part of Japan, an epidemiological survey of dogs suspected of having B. gibsoni infection was attempted using the polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Thirty-five of 115 such dogs (30.4%) were positive by PCR and/or ELISA. The 35 positive dogs consisted of 28 Tosa dogs, 4 American Pit Bull Terriers, and 3 mongrel dogs in Aomori, Fukushima, Ibaraki, Gunma, Chiba, Tokyo, Kanagawa, and Nagano Prefectures. The positive dogs had a significantly lower rate of tick exposure and a higher rate of bites by other dogs. Twenty-two of 35 B. gibsoni-positive dogs were infected with hemoplasma, and the rate of infection was significantly higher than...
Palavras-chave: Babesia gibsoni; Eastern Japan; Enzyme-linked immunosorbent assay; Hemoplasma; Polymerase chain reaction.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/924
Registros recuperados: 5
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