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Registros recuperados: 5
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Anti-Mycobacterium tuberculosis activity of fungus Phomopsis stipata BJM
Prince,Karina Andrade de; Sordi,Renata; Pavan,Fernando Rogério; Santos,Adolfo Carlos Barreto; Araujo,Angela R.; Leite,Sergio R.A.; Leite,Clarice Q. F..
Our purpose was to determine the anti-Mycobacterium tuberculosis activity of the metabolites produced by the endophitic fungus Phomopsis stipata (Lib.) B. Sutton, (Diaporthaceae), cultivated in different media. The antimycobacterial activity was assessed through the Resazurin Microtiter Assay (REMA) and the cytotoxicity test performed on macrophage cell line. The extracts derived from fungi grown on Corn Medium and Potato Dextrose Broth presented the smallest values of Minimum Inhibitory Concentration (MIC) and low cytotoxicity, which implies a high selectivity index. This is the first report on the chemical composition and antitubercular activity of metabolites of P. stipata, as well as the influence of culture medium on these properties.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phomopsis stipata; Antimycobacterial activity; Mycobacterium tuberculosis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100024
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Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping BJM
Miyata,Marcelo; Santos,Adolfo Carlos Barreto; Mendes,Natália Helena; Cunha,Eunice Atsuko; Melo,Fernando Augusto Fiúza de; Leite,Clarice Queico Fujimura.
We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA extraction; Mycobacterium tuberculosis; Thermolysis; CTAB.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200045
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Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil BJPS
Oltramari,Karine; Cardoso,Rosilene Fressati; Patussi,Eliana Valéria; Santos,Adolfo Carlos Barreto; Mikcha,Jane Martha Graton.
Food contamination caused by enteric pathogens is a major cause of diarrheal disease worldwide, resulting in high morbidity and mortality and significant economic losses. Bacteria are important agents of foodborne diseases, particularly diarrheagenic Escherichia coli. The present study assessed the genetic diversity and antimicrobial resistance of E. coli isolates from pasteurized milk processed in 21 dairies in northwestern State of Parana, Brazil. The 95 E. coli isolates were subjected to antimicrobial susceptibility testing according to the recommendations of the Clinical and Laboratory Standards Institute and assessed genotypically by Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR). The highest rate of resistance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pasteurized milk/processing; Pasteurized milk/bacterial typing; Pasteurized milk/contamination; Escherichia coli/genetic diversity; Escherichia coli/antimicrobial resistance; Food/microbiological analysis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200337
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High genetic diversity among Pseudomonas aeruginosa and Acinetobacter spp. isolated in a public hospital in Brazil BJPS
Siqueira,Vera Lúcia Dias; Cardoso,Rosilene Fressatti; Pádua,Rubia Andreia Falleiros de; Caleffi-Ferracioli,Katiany Rizzieri; Helbel,Cesar; Santos,Adolfo Carlos Barreto; Aoki,Elisabeth Eyko; Nakamura,Celso Vataru.
In Brazil and other regions of the world, Pseudomonas aeruginosa and Acinetobacter spp. have emerged as important agents of nosocomial infection and are commonly involved in outbreaks. The main objective of the present study was to evaluate the genetic relationship among P. aeruginosa and Acinetobacter spp. isolated from patients in a public university hospital in northwestern Paraná, Brazil, and report their antimicrobial resistance profile. A total of 75 P. aeruginosa and 94 Acinetobacter spp. isolates were phenotypically identified and tested for antibiotic susceptibility using automated methodology. Polymyxin B was tested by disk diffusion for P. aeruginosa. Metallo-β-lactamase (MBL) was detected using a disk approximation test. Genotyping was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa/antimicrobial resistance profile; Pseudomonas aeruginosa/genetic study; Acinetobacter spp./antimicrobial resistance profile; Acinetobacter spp./genetic study; Antimicrobial resistance; Bacterial typing.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100006
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Phenotypic and genotypic characterization of Rhodococcus equi isolated from sputum BJID
Silva,Paulo da; Santos,Adolfo Carlos Barreto; Sato,Daisy Nakamura; Silva,Jaqueline Otero; Medeiros,Marta Inês Cazentini; Carneiro,Ana Maria Machado; Leite,Sergio Roberto de Andrade; Leite,Clarice Queico Fujimura.
INTRODUCTION: Rhodococcus equi is an opportunistic pathogen, causing rhodococcosis, a condition that can be confused with tuberculosis. Often, without identifying M. tuberculosis, physicians initiate empiric treatment for tuberculosis. R. equi and M. tuberculosis have different susceptibility to drugs. Identification of R. equi is based on a variety of phenotypic, chromatographic, and genotypic characteristics. OBJECTIVE: This study aimed to characterize bacterial isolates from sputum samples suggestive of R. equi. METHODS: The phenotypic identification included biochemical assays; thin-layer chromatography (TLC) and polymerase chain reaction (PCR) were used for genotypic identification. RESULTS: Among 78 Gram-positive and partially acid-fast bacilli...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymerase chain reaction; Rhodococcus equi; Mycolic acids.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500001
Registros recuperados: 5
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