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Analysis of anthocyanins in strawberry varieties IPB - Escola Superior Agrária
Lopes-da-Silva, M.F.; Escribano-Bailón, M.T.; González-Paramás, A.M.; Rivas-Gonzalo, J.C.; Santos-Buelga, Celestino.
Comissão Europeia (Fundo Social Europeu) e Governo Português através do Programa PRODEP (III) - ref.ª 5.3/N/199.006/00-Doutoramento.
Tipo: ConferenceObject Palavras-chave: Strawberry varieties; Anthocyanin composition; Cyanidin derivatives; Pelargonidin derivatives.
Ano: 2002 URL: http://hdl.handle.net/10198/5767
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Anthocyanin composition and related pigments in strawberry IPB - Escola Superior Agrária
Lopes-da-Silva, M.F.; Escribano-Bailón, M.T.; Martin-Lopez, P.; Rivas-Gonzalo, J.C.; Santos-Buelga, Celestino.
The anthocyanin composition of the strawberry has been object of various studies, but it is still not fully characterized. It is well known the presence af pelarganidin 3 - glucoside (Pg 3 -gluc) as major anthocyanin, usually accompanied of smaller proportions of pelargonidin 3- rutinoside (Pg 3 -rut) and cyanidin 3-glucosid e (Cy. 3- gluc).
Tipo: ConferenceObject Palavras-chave: Strawberry cultivars; Anthocyanin composition; Minor pigments; C-C linked anthocyanin and flavanol residues.
Ano: 2004 URL: http://hdl.handle.net/10198/5969
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Anthocyanin pigments in strawberry IPB - Escola Superior Agrária
Lopes-da-Silva, M.F.; Escribano-Bailón, M.T.; Perez-Alonso, J.J.; Rivas-Gonzalo, J.C.; Santos-Buelga, Celestino.
The anthocyanin composition was analysed in strawberry fruits from five different cultivars (cv. Eris, Oso Grande, Carisma, Tudnew and Camarosa). Twenty-five defined anthocyanin pigments were detected, most of them containing Pelargonidin (Pg) as aglycone; some cyanidin (Cy) derivatives were also found. Glucose and rutinose were the usual substituting sugars, although arabinose and rhamnose were also tentatively identified; some minor anthocyanins showed acylation with aliphatic acids. A relevant aspect was the detection of anthocyanin-derived pigments, namely 5-carboxypyranopelargonidin-3-glucoside and four condensed pigments containing C–C linked anthocyanin (Pg) and flavanol (catechin and afzelechin) residues. Total anthocyanin content ranged between...
Tipo: Article Palavras-chave: Anthocyanins; Strawberry; Pelargonidin; Cyanidin-derived pigments; Anthocyanin-derived pigments.
Ano: 2007 URL: http://hdl.handle.net/10198/4556
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Chemical composition of wild edible mushrooms and antioxidant properties of their water soluble polysaccharidic and ethanolic fractions IPB - Escola Superior Agrária
Vaz, Josiana A.; Barros, Lillian; Martins, Anabela; Santos-Buelga, Celestino; Vasconcelos, M. Helena; Ferreira, Isabel C.F.R..
Mushrooms have become attractive as functional foods and as a source of physiologically beneficial bioactive compounds. Herein, we describe and compare the chemical constituents (phenolic compounds, macronutrients, sugars, fatty acids, tocopherols and ascorbic acid) of four wild edible mushrooms widely appreciated in gastronomy: Armillaria mellea (Vahl) P. Kumm, Calocybe gambosa (Fr.) Donk, Clitocybe odora (Fr.) P. Kumm., Coprinus comatus (O.F. Müll.) Pers. Furthermore, the antioxidant activity of their water soluble polysaccharidic and ethanolic fractions was studied by three different in vitro assays. Coprinus comatus revealed the highest concentrations of sugars (43.23 g/100 g dry weight), PUFA (77.46%), phenolic compounds (45.02 mg/Kg), tocopherols...
Tipo: Article Palavras-chave: Wild edible mushrooms; Bioactive compounds; Phenolic compounds; Polysaccharides; Chemical composition.
Ano: 2011 URL: http://hdl.handle.net/10198/3881
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Determination of phenolic compounds in Portuguese wild mushrooms IPB - Escola Superior Agrária
Barros, Lillian; Dueñas, Montserrat; Ferreira, Isabel C.F.R.; Santos-Buelga, Celestino.
Tipo: ConferenceObject
Ano: 2008 URL: http://hdl.handle.net/10198/3432
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Flavan-3-ol composition of strawberries IPB - Escola Superior Agrária
Lopes-da-Silva, M.F.; Santos-Buelga, Celestino; Rivas-Gonzalo, J.C.; Escribano-Bailón, M.T..
Comissão Europeia (Fundo Social Europeu) e Governo Português através do Programa PRODEP (III) - ref.ª 5.3/N/199.006/00-Doutoramento.
Tipo: ConferenceObject Palavras-chave: Strawberry varieties; Flavan-3-ol composition; HPLC-DAD-MS analysis; Catechin-derived flavanols.
Ano: 2004 URL: http://hdl.handle.net/10198/5766
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Flavanol-anthocyanin condensed pigments in plant extracts IPB - Escola Superior Agrária
González-Paramás, A.M.; Lopes-da-Silva, M.F.; Martin-Lopez, P.; Macz-Pop, G.; Gonzalez-Manzano, S.; Alcalde-Eon, C.; Perez-Alonso, J.J.; Escribano-Bailón, M.T.; Rivas-Gonzalo, J.C.; Santos-Buelga, Celestino.
Pigments resulting from the direct condensation of anthocyanins and flavanols are usually associated with reactions taking place during processing and storage of plant-derived foods and beverages and have been particularly studied in aged red wines. In this paper, small amounts of flavanol-anthocyanin condensed pigments are found in different plant extracts. Structures are suggested for 10 such condensed pigments detected in extracts of strawberry, runner beans, purple corn and grape skins, based on their MS" fragmentation patterns, following analyses by electrospray tandem mass spectrometry. All of them correspond to dimers containing a flavan-3-ol [either (epi)afzelechin, (epi)catechin or (epi)gallocatechin] as the upper unit carbon-carbon linked to a...
Tipo: Article Palavras-chave: Flavanol-anthocyanin; Condensed pigments; Strawberry; Runner bean; Purple corn; Grape skin.
Ano: 2006 URL: http://hdl.handle.net/10198/5857
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Identification of anthocyanin pigments in strawberry (cv Camarosa) by LC using DAD and ESI-MS detection IPB - Escola Superior Agrária
Lopes-da-Silva, M.F.; Pascual-Teresa, Sónia de; Rivas-Gonzalo, J.C.; Santos-Buelga, Celestino.
New high performance liquid chromatography (HPLC) conditions were developed for the separation of strawberry anthocyanins that provided a good resolution of peaks at a low flow rate compatible with the requirements of the mass spectrometry (MS) detector. A strawberry extract was fractionated by column chromatography and simple fractions containing basically anthocyanins were obtained, making their analysis by HPLC possible using on-line photodiode array detection and MS. Information on the identity of the major and some secondary anthocyanins in strawberry was obtained froth their retention characteristics, UV-visible spectra and mass spectra. The presence in strawberry of the previously reported cyanidin 3-glucoside, pelargonidin 3-glucoside, pelargonidin...
Tipo: Article Palavras-chave: Strawberry; Anthocyanins; Liquid chromatography-mass spectrometry; Cyanidin; Pelargonidin.
Ano: 2002 URL: http://hdl.handle.net/10198/5971
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Influence of mycorrhizal symbiosis in antioxidant potential of fungi and seedlings. IPB - Escola Superior Agrária
Reis, Filipa S.; Ferreira, Isabel C.F.R.; Barros, Lillian; Santos-Buelga, Celestino; Martins, Anabela.
Ectomycorrhizal (ECM) symbiosis plays a major role in nutrient cycling and the functioning of forest ecosystems. Trees with well developed ectomycorrhizas are more resistant to environmental stresses such as drought and to biotic stresses such as root pathogens [1]. The establishment of ECM symbiosis is triggered by signals produced by both partners. These signals lead to morphological changes and a complex development of specific structures in both the plant root and the fungus [2].
Tipo: ConferenceObject
Ano: 2011 URL: http://hdl.handle.net/10198/6202
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Mycorrhizal induction of phenolic compounds and antioxidant properties of fungi and seedlings during the early steps of symbiosis. IPB - Escola Superior Agrária
Reis, Filipa S.; Ferreira, Isabel C.F.R.; Barros, Lillian; Santos-Buelga, Celestino; Martins, Anabela.
Ectomycorrhizal (ECM) symbiosis plays a major role in nutrient cycling and the functioning of forest ecosystems. Trees with well developed ectomycorrhizas are more resistant to environmental stresses such as drought and to biotic stresses such as root pathogens. The establishment of ECM symbiosis is triggered by signals produced by both partners. These signals lead to morphological changes and a complex development of specific structures in both the plant root and the fungus. The development of the ECM fungi, Paxillus involutus and Pisolithus arhizus, in presence and absence of the symbiont – Pinus pinaster- was evaluated, as well as their antioxidant properties and phenolic compounds composition in response to the symbiotic association. ECM fungi grew...
Tipo: Article Palavras-chave: Antioxidant activity; Ectomycorrhizal fungi; Phenolic compounds; Pinus pinaster seedlings; Symbiosis.
Ano: 2011 URL: http://hdl.handle.net/10198/6229
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Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method IPB - Escola Superior Agrária
Barros, Lillian; Correia, Daniela M.; Ferreira, Isabel C.F.R.; Baptista, Paula; Santos-Buelga, Celestino.
Individual tocopherol profile of five Agaricus mushroom species, widely consumed in Portugal, was obtained by a normal-phase high-performance liquid chromatography (NP-HPLC). It was used a simple solid–liquid extraction procedure without saponification step and the chromatographic separation was achieved using a YMC-Pack Polyamine II column using an isocratic elution with hexane/ethyl acetate (70:30, v/v) at a flow rate of 1.0 mL/min. The effluent was monitored by a fluorescence detector. All the compounds were separated in a period of time of 30 min. The method proved to be sensitive, reproducible and accurate, allowing the determination of tocopherols.
Tipo: Article Palavras-chave: Vitamin E; HPLC Analysis; Mushrooms.
Ano: 2008 URL: http://hdl.handle.net/10198/838
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Phenolic acids determination by HPLC-DAD-ESI/MS in sixteen different portuguese wild mushrooms species IPB - Escola Superior Agrária
Barros, Lillian; Dueñas, Montserrat; Ferreira, Isabel C.F.R.; Baptista, Paula; Santos-Buelga, Celestino.
Analysis of phenolic compounds in sixteen Portuguese wild mushrooms species has been carried out by high-performance liquid chromatography coupled to photodiode array detector and mass spectrometer (HPLC–DAD–ESI/MS). No flavonoids were detected in the analysed samples, but diverse phenolic acids namely protocatechuic, p-hydroxybenzoic and p-coumaric acids, and two vanillic acid isomers were found and quantified. A related non-phenolic compound, cinnamic acid, was also detected in some samples, being the only compound found in Cantharellus cibarius (14.97 mg/kg, dry matter), Lycoperdon perlatum (14.36 mg/kg) and Macrolepiota procera (21.53 mg/kg). p-Hydroxybenzoic acid was found in the majority of the samples, being the most abundant compound in Agaricus...
Tipo: Article Palavras-chave: Wild mushrooms; Phenolic compounds; HPLC–DAD–ESI/MS.
Ano: 2009 URL: http://hdl.handle.net/10198/2650
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Phenolic acids profile and antioxidant properties of wild edible mushrooms from North-eastern Portugal IPB - Escola Superior Agrária
Barros, Lillian; Sousa, Maria João; Martins, Anabela; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R..
Tipo: ConferenceObject
Ano: 2010 URL: http://hdl.handle.net/10198/3349
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Phenolic composition and antioxidant activity of Portuguese wild edible greens IPB - Escola Superior Agrária
Barros, Lillian; Ferreira, Isabel C.F.R.; Carvalho, Ana Maria; Dueñas, Montserrat; Santos-Buelga, Celestino.
Tipo: ConferenceObject
Ano: 2010 URL: http://hdl.handle.net/10198/3502
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Phenolic compounds and antioxidant activity of fruiting body and spores of wild Ganoderma lucidum. IPB - Escola Superior Agrária
Heleno, Sandrina A.; Barros, Lillian; Martins, Anabela; Queiroz, Maria João R.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R..
Ganoderma lucidum, a woody Basidiomycotina mushroom belonging to the family of Ganodermaceae of Polyporales, is not edible but extensively used in folk medicine for longevity and health promotion. Some pharmacological properties have been related to its capacity to lower the risk of cancer, liver, and heart diseases and to boost the immune system [1]. The beneficial health properties of Ganoderma are attributed to a wide variety of bioactive components present in the fruiting body, mycelium, and spores, mainly polysaccharides and triterpenes [2]. In the present work other bioactive molecules (phenolic compounds) were analysed by high-performance liquid chromatography coupled to photodiode array detection and mass spectrometry (HPLC-DAD-MS), in wild...
Tipo: ConferenceObject
Ano: 2011 URL: http://hdl.handle.net/10198/6094
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Stability of pelargonidin 3-glucoside in model solutions IPB - Escola Superior Agrária
Lopes-da-Silva, M.F.; Escribano-Bailón, M.T.; Rivas-Gonzalo, J.C.; Santos-Buelga, Celestino.
Comissão Europeia (Fundo Social Europeu) e Governo Português através do Programa PRODEP (III) - ref.ª 5.3/N/199.006/00-Doutoramento.
Tipo: ConferenceObject Palavras-chave: Pelargonidin 3-glucoside; Model solutions; Color change; Storage; Anthocyanin breakdown products; Aerobic/anaerobic conditions.
Ano: 2004 URL: http://hdl.handle.net/10198/5765
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Stability of pelargonidin 3-glucoside in model solutions in the presence and absence of flavanols IPB - Escola Superior Agrária
Lopes-da-Silva, M.F.; Escribano-Bailón, M.T.; Santos-Buelga, Celestino.
The stability of pelargonidin 3-g1ucoside (Pg3g1uc), the main anthocyanin in strawberry, has been studied in model citrate solutions (PH 3.5, 25°C) in the absence and presence flavanols (catechin and procyanidin B3) and/or oxygen. Changes in the solutions were monitorized by HPLC-DAD/MS and UV-visible spectra.
Tipo: Article Palavras-chave: Pelargonidin; Catechin; Procyanidin B3; Anthocyanin stability; Xanthylium pigments; Strawberry.
Ano: 2007 URL: http://hdl.handle.net/10198/5970
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Targeted metabolites analysis in wild Boletus species IPB - Escola Superior Agrária
Heleno, Sandrina A.; Barros, Lillian; Sousa, Maria João; Martins, Anabela; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R..
In European countries, the edible mushrooms from the Boletus genus are the most frequently harvested of all the forest species gathered in the wild. Their popularity is mainly due to their sensory qualities, in particular aroma, taste and texture. In the present work, a targeted metabolites analysis was performed in six wild Boletus species. The analysis of primary metabolites revealed proteins, carbohydrates, fatty acids, mainly linoleic acid, sugars, mainly mannitol and trehalose, and vitamins (tocopherols and ascorbic acid). Secondary metabolites, such as phenolic acids, were also identified and quantified, and correlated to Boletus antioxidant properties including free radical scavenging activity, reducing power and lipid peroxidation inhibition. As a...
Tipo: Article Palavras-chave: Wild mushrooms; Boletus sp.; Primary/secondary metabolites; Bioactivity.
Ano: 2011 URL: http://hdl.handle.net/10198/6028
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Towards the antioxidant and chemical characterization of mycorrhizal mushrooms from Northeast Portugal IPB - Escola Superior Agrária
Reis, Filipa S.; Heleno, Sandrina A.; Barros, Lillian; Sousa, Maria João; Martins, Anabela; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R..
Mushrooms are widely appreciated all over the world for their nutritional properties and pharmacological value as sources of important bioactive compounds. Mycorrhizal macrofungi associate with plant roots constituting a symbiotic relationship. This symbiosis could influence the production of secondary metabolites, including bioactive compounds. We focused on the evaluation of antioxidant potential and chemical composition of mycorrhizal mushrooms species from Northeast Portugal: Amanita caesarea, Amanita muscaria, Amanita pantherina, Chroogomphus fulmineus, Cortinarius anomalus, Cortinarius collinitus, Cortinarius violaceus, Lactarius quietus, Lactarius volemus, Russula sardonia, Suillus luteus and Tricholoma ustale. A similar profile of metabolites was...
Tipo: Article Palavras-chave: Mycorrhizal mushrooms; Antioxidant activity; Nutrients; Bioactive compounds.
Ano: 2011 URL: http://hdl.handle.net/10198/6251
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Use of HPLC-DAD-ESI/MS to profile phenolic compounds in edible wild greens from Portugal. IPB - Escola Superior Agrária
Barros, Lillian; Dueñas, Montserrat; Ferreira, Isabel C.F.R.; Carvalho, Ana Maria; Santos-Buelga, Celestino.
Fruits and vegetables are good sources of a large number of antioxidant compounds; moreover, in some Mediterranean areas traditional wild greens are responsible for a significant percentage of total dietary antioxidant intakes. Asparagus acutifolius L. (wild asparagus), Bryonia dioica Jacq. (white bryony) and Tamus communis L. (black bryony) are important examples of those edible wild greens widely consumed. This study aimed to determine the phenolic profile and composition of edible vernal early shoots in spring of those species. The analysis was performed by high performance liquid chromatography coupled to photodiode array detector and mass spectrometry. Wild asparagus and black bryony revealed glycosides of flavonols as main phenolic compounds, while...
Tipo: Article Palavras-chave: Wild edible greens; Phenolic compounds; HPLC-DAD-ESI/MS.
Ano: 2011 URL: http://hdl.handle.net/10198/6026
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