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Registros recuperados: 23
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Analysis and quantification of flavonoidic compounds from Portuguese olive (Olea europeae L.) leaf cultivars IPB - Escola Superior Agrária
Meirinhos, J.; Silva, B.M.; Valentão, P.; Seabra, R.M.; Pereira, J.A.; Dias, A.; Andrade, P.B.; Ferreres, F..
Twenty three samples of 18 Portuguese olive leaf cultivars were analysed by a reversed-phase HPLC/DAD procedure and eight flavonoidic compounds were identified and quantified (luteolin 7,40-O-diglucoside, luteolin 7-O-glucoside, rutin, apigenin 7-O-rutinoside, luteolin 40-O-glucoside, luteolin, apigenin and diosmetin). Luteolin 7,40-O-diglucoside and luteolin 40-O-glucoside were identified by HPLC/DAD/MS/MS – ESI. The studied olive leaf samples showed a common phenolic pattern, in which luteolin 40-O-glucoside was almost always the major compound
Tipo: Article Palavras-chave: Olea europaea L.; Olive leaves; Olive cultivars; HPLC-DAD/MS/MS/ESI; Flavonoids.
Ano: 2005 URL: http://hdl.handle.net/10198/728
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Chemical assessment and in vitro antioxidant capacity of Ficus carica latex IPB - Escola Superior Agrária
Oliveira, A.P.; Silva, L.R.; Ferreres, F.; Pinho, P. Guedes de; Valentão, P.; Silva, B.M.; Pereira, J.A.; Andrade, P.B..
Ficus species possess latex-like material within their vasculatures, affording protection and selfhealing from physical attacks. In this work, metabolite profiling was performed on Ficus carica latex. Volatiles profile was determined by HS-SPME/GC-IT-MS, with 34 compounds being identified, distributed by distinct chemical classes: 5 aldehydes, 7 alcohols, 1 ketone, 9 monoterpenes, 9 sesquiterpenes and 3 other compounds. Sesquiterpenes constituted the most abundant class in latex (ca. 91% of total identified compounds). Organic acids composition was also characterized, by HPLC-UV, and oxalic, citric, malic, quinic, shikimic and fumaric acids were determined. Malic and shikimic acids were present in higher amounts (ca. 26%, each). The antioxidant potential...
Tipo: Article Palavras-chave: Ficus carica latex; Volatiles; Organic acids; Antioxidant potential; Acetylcholinesterase inhibition.
Ano: 2010 URL: http://hdl.handle.net/10198/3116
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Comparative study on free amino acids composition of wild edible mushroom species IPB - Escola Superior Agrária
Ribeiro, Bárbara; Andrade, P.B.; Silva, B.M.; Baptista, Paula; Seabra, R.M.; Valentão, P..
A comparative study on the amino acid composition of 11 wild edible mushroom species (Suillus bellini, Suillus luteus, Suillus granulatus, Tricholomopsis rutilans, Hygrophorus agathosmus, Amanita rubescens, Russula cyanoxantha, Boletus edulis, Tricholoma equestre, Fistulina hepatica, and Cantharellus cibarius) was developed. To define the qualitative and quantitative profiles, a derivatization procedure with dabsyl chloride was performed, followed by HPLC-UV-vis analysis. Twenty free amino acids (aspartic acid, glutamic acid, asparagine, glutamine, serine, threonine, glycine, alanine, valine, proline, arginine, isoleucine, leucine, tryptophan, phenylalanine, cysteine, ornithine, lysine, histidine, and tyrosine) were determined. B. edulis and T. equestre...
Tipo: Article Palavras-chave: Wild edible mushrooms; Free amino acids; Nutritional value.
Ano: 2008 URL: http://hdl.handle.net/10198/4407
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Determination of low molecular weight volatiles in Ficus carica using HS-SPME and GC/FID IPB - Escola Superior Agrária
Oliveira, A.P.; Silva, L.R.; Andrade, P.B.; Valentão, P.; Silva, B.M.; Pereira, J.A.; Pinho, P. Guedes de.
Ficus carica L. is one of the earliest cultivated fruit trees, having an important consumption in Mediterranean countries. In this work, the volatile compound profiles of two characteristic Portuguese white varieties (‘‘Pingo de Mel” and ‘‘Branca Tradicional”) was determined by HS-SPME and GC/FID. Leaves, pulps and peels, submitted to freezing and lyophilisation treatments, were analysed. The two varieties presented a similar profile composed of eight volatile compounds: acetaldehyde, ethyl acetate, methanol, ethanol, hexanal, limonene, (E)-2-hexenal and octanal. The total volatile content was different among the vegetal materials, following the order leaves > peels > pulps. Methanol and ethanol are the major compounds in all samples. The developed...
Tipo: Article Palavras-chave: Ficus carica; Volatile compounds; HS-SPME; GC/FID.
Ano: 2010 URL: http://hdl.handle.net/10198/3115
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Development and evaluation of an HPLC/DAD method for the analysis of phenolic compounds from olive fruit IPB - Escola Superior Agrária
Vinha, A.F.; Silva, B.M.; Andrade, P.B.; Seabra, R.M.; Pereira, J.A.; Oliveira, M.B.P.P..
A new HPLC/DAD methodology for separating nine phenolic compounds is described. This methodology is applied to the definition of qualitative and quantitative profiles of three Portuguese olive fruit cultivars (Cobrançosa, Madural and Verdeal). Two different extraction methods were needed for the complete definition of their profiles, one of them including a Sep-pack C18 cleaning step. The chromatographic separation was achieved using a Spherisorb ODS2 (25.0 × 0.46 cm; 5 μm, particle size) column. The solvent system used was a gradient of water-formic acid (19:1) and methanol, with a flow rate of 0.9 mL/min. The detection limit values for phenolic compounds were between 0.04 and 4.32 μg/mL and the method was precise. As a general rule, the recovery values...
Tipo: Article Palavras-chave: High performance liquid chromatography; Diodes; Phenols.
Ano: 2002 URL: http://hdl.handle.net/10198/723
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Development of an HPLC/DAD method for determination of phenolic profile in portuguese olive fruits. IPB - Escola Superior Agrária
Vinha, A.; Andrade, P.B.; Silva, B.M.; Pereira, J.A.; Valentão, P.; Seabra, R.M.; Oliveira, M.B.P.P..
Tipo: ConferenceObject
Ano: 2001 URL: http://hdl.handle.net/10198/5553
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Effect of the conservation procedure on the contents of phenolic compounds and organic acids in Chanterelle (Cantharellus cibarius) Mushroom IPB - Escola Superior Agrária
Valentão, P.; Andrade, P.B.; Rangel, Joana; Ribeiro, Bárbara; Silva, B.M.; Baptista, Paula; Seabra, R.M..
To check the influence of the conservation procedure in the chemical composition of chanterelle mushroom, phenolic compounds and organic acids of samples preserved under four different conditions (drying, freezing, conservation in olive oil and in vinegar) were determined. Phenolics and organic acids were analyzed by HPLC-DAD and HPLC-UV, respectively. The results showed that chanterelle is characterized by the presence of six phenolic compounds (3-, 4-, and 5-O-caffeoylquinic acid, caffeic acid, p-coumaric acid, and rutin) and five organic acids (citric, ascorbic, malic, shikimic, and fumaric acids). Samples preserved in olive oil also exhibited hydroxytyrosol, tyrosol, luteolin, and apigenin, whereas conservation in vinegar led to the detection of...
Tipo: Article Palavras-chave: Chanterelle; Cantharellus cibarius; Conservation; Phenolic compounds; Organic acids.
Ano: 2005 URL: http://hdl.handle.net/10198/940
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Evaluation of free radical-scavenging and antihemolytic activities of quince (Cydonia oblonga) leaf: A comparative study with green tea (Camellia sinensis) IPB - Escola Superior Agrária
Costa, R.M.; Magalhães, A.S.; Pereira, J.A.; Andrade, P.B.; Valentão, P.; Carvalho, M.; Silva, B.M..
This study aimed to determine the phenolic profile and to investigate the antioxidant potential of quince (Cydonia oblonga) leaf, comparing it with green tea (Camellia sinensis). For these purposes, methanolic extracts were prepared and phenolics content of quince leaf was determined by HPLC/UV. The antioxidant properties were assessed by Folin–Ciocalteu reducing capacity assay and by the ability to quench the stable free radical 2,20-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,20-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes. 5-O-Caffeoylquinic acid was found to be the major phenolic compound in quince leaf extract. Quince leaf exhibited a significantly higher reducing power than green tea...
Tipo: Article Palavras-chave: Antioxidant activity; Cydonia oblonga; Quince leaf; Hemolysis; Phenolic compounds; Camellia sinensis.
Ano: 2009 URL: http://hdl.handle.net/10198/3044
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Ficus carica L.: metabolic and biological screening. IPB - Escola Superior Agrária
Oliveira, A.; Valentão, P.; Pereira, J.A.; Silva, B.M.; Tavares, F.; Andrade, P.B..
Ficus carica L. is one of the earliest cultivated fruit trees. In this work, metabolite profiling was performed on the leaves, pulps and peels of two Portuguese white varieties of F. carica (Pingo de Mel and Branca Tradicional). Phenolics and organic acids profiles were determined by HPLC/DAD and HPLC/UV, respectively. All samples presented a similar phenolic profile composed by 3-O- and 5-O-caffeoylquinic acids, ferulic acid, quercetin-3-O-glucoside, quercetin-3-O-rutinoside, psoralen and bergapten. 3-O-Caffeoylquinic acid and quercetin-3-O-glucoside are described for the first time in this species. Leaves’ organic acids profile presented oxalic, citric, malic, quinic, shikimic and fumaric acids, while in pulps and peels quinic acid was absent. The...
Tipo: Article Palavras-chave: Ficus carica L.; Phenolic compounds; Organic acids; Antioxidant; Acetylcholinesterase inhibition; Antimicrobial activity.
Ano: 2009 URL: http://hdl.handle.net/10198/3111
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Flavonóides em folhas de cultivares portuguesas de oliveira. IPB - Escola Superior Agrária
Meirinhos, J.; Silva, B.M.; Valentão, P.; Seabra, R.M.; Pereira, J.A.; Dias, A.; Andrade, P.B.; Ferreres, F..
Tipo: ConferenceObject
Ano: 2005 URL: http://hdl.handle.net/10198/5561
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Free amino acids of Tronchuda Cabbage (Brassica oleracea L. Var. costata DC): influence of leaf position (internal or external) and collection time IPB - Escola Superior Agrária
Oliveira, Andreia P.; Pereira, D.M.; Andrade, P.B.; Valentão, P.; Sousa, Carla; Pereira, J.A.; Bento, Albino; Rodrigues, M.A.; Seabra, R.M.; Silva, B.M..
The free amino acid profile of 18 samples of tronchuda cabbage (Brassica oleracea L. var. costata DC) leaves, harvested at three different months, was determined by HPLC/UV−vis. The tronchuda cabbage leaves total free amino acid content varied from 3.3 to 14.4 g/kg fresh weight. Generally, arginine was the major compound, followed by proline, threonine, glutamine, cysteine, and glutamic acid. This study indicates that free amino acids are not similarly distributed: in external leaves, proline and arginine were the major free amino acids, while in internal ones, arginine was the main free amino acid, followed by threonine, glutamine, and cysteine. Significant differences were observed for valine, proline, arginine, leucine, cysteine, lysine, histidine, and...
Tipo: Article Palavras-chave: Brassica oleracea L. var. costata DC; Tronchuda cabbage; Internal and external leaves; Free amino acids.
Ano: 2008 URL: http://hdl.handle.net/10198/716
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Further insights into the latex metabolite profile of Ficus carica IPB - Escola Superior Agrária
Oliveira, A.P.; Silva, L.R.; Andrade, P.B.; Valentão, P.; Silva, B.M.; Gonçalves, Rui; Pereira, J.A.; Pinho, P. Guedes de.
Latex is a sticky emulsion that exudes upon damage from specialized canals from several plants. It contains several biologically active compounds, such as phytosterols, fatty acids, and amino acids. In plants, these compounds are involved in the interaction between plants, insects, and the environment. Despite its chemical, biological, and ecological importance, Ficus carica latex is still poorly studied. To improve the knowledge on the metabolite profile of this matrix, a targeted metabolite analysis was performed in a representative sample from F. carica latex. Seven phytosterols were determined by gas chromatography-ion trap mass spectrometry (GC-ITMS) and high-performance liquid chromatography coupled to diode array detection (HPLC-DAD), with...
Tipo: Article Palavras-chave: Ficus carica latex; Phytosterols; Free and combined fatty acids; Free amino acids.
Ano: 2010 URL: http://hdl.handle.net/10198/4561
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Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. IPB - Escola Superior Agrária
Carvalho, M.; Ferreira, P.J.; Mendes, V.S.; Silva, R.; Pereira, J.A.; Jerónimo, C.; Silva, B.M..
Several studies suggest that regular consumption of nuts, mostly walnuts, may have beneficial effects against oxidative stress mediated diseases such as cardiovascular disease and cancer. Walnuts contain several phenolic compounds which are thought to contribute to their biological properties. The present study reports the total phenolic contents and antioxidant properties of methanolic and petroleum ether extracts obtained from walnut (Juglans regia L.) seed, green husk and leaf. The total phenolic contents were determined by the Folin–Ciocalteu method and the antioxidant activities assessed by the ability to quench the stable free radical 2,20-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,20-azobis( 2-amidinopropane) dihydrochloride...
Tipo: Article Palavras-chave: Juglans regia L.; Leaf; Green husk; Walnut; Antiproliferative activity; Antioxidant.
Ano: 2010 URL: http://hdl.handle.net/10198/3113
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Organic acids composition of Cydonia oblonga Miller leaf IPB - Escola Superior Agrária
Oliveira, Andreia P.; Pereira, J.A.; Andrade, P.B.; Valentão, P.; Seabra, R.M.; Silva, B.M..
Organic acid profiles of 36 Cydonia oblonga Miller leaf samples, from three different geographical origins of northern (Bragança and Carrazeda de Ansiães) and central Portugal (Covilhã), harvested in three collection months (June, August and October of 2006), were determined by HPLC/UV (214 nm). Quince leaves presented a common organic acid profile, composed of six constituents: oxalic, citric, malic, quinic, shikimic and fumaric acids. C. oblonga leaves total organic acid content varied from 1.6 to 25.8 g/kg dry matter (mean value of 10.5 g/kg dry matter). Quinic acid was the major compound (72.2%), followed by citric acid (13.6%). Significant differences were found in malic and quinic acids relative abundances and total organic acid contents according...
Tipo: Article Palavras-chave: Cydonia oblonga Miller; Quince leaves; Organic acids; Variability.
Ano: 2008 URL: http://hdl.handle.net/10198/718
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Phenolic composition of Cydonia oblonga Miller leaf. IPB - Escola Superior Agrária
Silva, B.M.; Oliveira, A.P.; Pereira, J.A.; Valentão, P.; Seabra, R.M.; Andrade, P.B..
Phenolic profile of 36 Cydonia oblonga Miller leaf samples, from 3 different geographical origins of Portugal, harvested in 3 collection months, was determined by HPLC/DAD. Quince leaf presented a common profile composed by 9 constituents: 3-0-, 4-0- and 5-0-caffeoylquinic acids, 3,5-0-dicaffeoylquinic acid, quercetin-3-0 galactoside, quercetin-3-0-rutinoside, kaempferol-3-0-glycoside, kaempferol-3-0-g1ucoside and kaempferol-3-0-rutinoside. C. oblonga leaf total content varied from 4.9 to 16.5 g/kg of dry matter, indicating that this leaf can be used as a good and cheap source of bioactive constituents. 5-0-caffeoylquinic acid was the major phenolic compound (36.2%). Significantly differences were found in 3-0-caffeoylquinic and 3,5-0-dicaffeoylquinic...
Tipo: ConferenceObject
Ano: 2008 URL: http://hdl.handle.net/10198/6046
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Phenolic compounds in three portuguese olive fruits varieties. IPB - Escola Superior Agrária
Vinha, A.; Andrade, P.B.; Silva, B.M.; Pereira, J.A.; Valentão, P.; Seabra, R.M.; Oliveira, M.B.P.P..
Tipo: ConferenceObject
Ano: 2001 URL: http://hdl.handle.net/10198/5544
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Phenolic profile and antioxidative properties of the beefsteak fungus Fistulina hepatica IPB - Escola Superior Agrária
Silva, B.M.; Ribeiro, Bárbara; Rangel, Joana; Baptista, Paula; Seabra, R.M..
The phenolic composition of the edible beefsteak fungus Fistulina hepatica was determined by HPLC/DAD. The results showed a profile composed by caffeic, p-coumaric and ellagic acids, hyperoside and quercetin. Ellagic acid was the main compound in this species. Beefsteak fungus was also investigated for its capacity to act as a scavenger of DPPH radical and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid). Good results were obtained against DPPH and superoxide radicals and hypochlorous acid but a prooxidant effect was observed for hydroxyl radical.
Tipo: ConferenceObject Palavras-chave: Fistulina hepatica; Phenolic composition; Antioxidative properties.
Ano: 2006 URL: http://hdl.handle.net/10198/5721
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Phenolic profile of Cydonia oblonga Miller leaves IPB - Escola Superior Agrária
Oliveira, Andreia P.; Pereira, J.A.; Andrade, P.B.; Valentão, P.; Seabra, R.M.; Silva, B.M..
Cydonia oblonga Miller leaves phenolic compounds were analyzed by reversed-phase HPLC/DAD and HPLC/UV. Qualitative and quantitative analysis of phenolics were carried out in a total of 36 samples of quince leaves from three different geographical origins of Northern (Bragança and Carrazeda de Ansiães) and Central Portugal (Covilhã) and three collection months (June, August, and October of 2006). These leaves presented a common phenolic profile composed by nine compounds: 3-O-, 4-O- and 5-O-caffeoylquinic acids, 3,5-O-dicaffeoylquinic acid, quercetin-3-Ogalactoside, quercetin-3-O-rutinoside, kaempferol-3-O-glycoside, kaempferol-3-O-glucoside, and kaempferol-3-O-rutinoside. 5-O-caffeoylquinic acid was the major phenolic compound (36.2%), followed by...
Tipo: Article Palavras-chave: Cydonia oblonga Miller; Quince leaves; Phenolic compounds.
Ano: 2007 URL: http://hdl.handle.net/10198/721
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Phenolic profiles of Portuguese olive fruits (Olea europaea L.): influence of cultivar and geographical origin IPB - Escola Superior Agrária
Vinha, A.F.; Ferreres, F.; Silva, B.M.; Valentão, P.; Gonçalves, Artur; Pereira, J.A.; Oliveira, M.B.P.P.; Seabra, R.M.; Andrade, P.B..
The phenolic compounds present in 29 samples of olive fruits were analysed by reversed-phase HPLC/DAD and/or HPLC-DAD/ ESI-MS/MS. All samples were collected during the normal picking period for olive oil production, in north and central Portugal, and were obtained from 18 different olive cultivars. Two different extraction methods were necessary for the complete quantification of phenolic compounds, a methanolic extraction and an extraction which included a solid-phase extraction (SPE) cleaning step. The analyses showed that all samples presented a similar profile, which included at least six identified phenolic compounds: hydroxytyrosol, luteolin 7-O-glucoside, oleuropein, rutin, apigenin 7-O-glucoside and luteolin. Several samples also contained...
Tipo: Article Palavras-chave: Olea europaea L.; Olive fruit; Portuguese olive cultivars; Anthocyanins; Phenolics; HPLC-DAD; HPLC-DAD/MS/MS/ESI.
Ano: 2005 URL: http://hdl.handle.net/10198/727
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Phytochemical profiles and inhibitory effect on free radical-induced human erythrocyte damage of Dracaena draco leaf: A potential novel antioxidant agent IPB - Escola Superior Agrária
Santos, R.P.; Mendes, L.S.; Silva, B.M.; Pinho, P. Guedes de; Valentão, P.; Andrade, P.B.; Pereira, J.A.; Carvalho, M..
The present study reports for the first time the metabolite profile and antioxidant activity of aqueous extract obtained from Dracaena draco L. leaf. Volatiles profile was determined by HS-SPME/GC-IT-MS, with 34 compounds being identified, distributed by distinct chemical classes: 2 alcohols, 5 aldehydes, 16 carotenoid derivatives and 8 terpenic compounds. Carotenoid derivative compounds constituted the most abundant class in leaf (representing 45% of total identified compounds). Phenolics profile was determined by HPLC/DAD and 9 constituents were identified: 2 hydroxycinnamic acid derivatives – 5- O-caffeoylquinic and 3,5-O-dicaffeoylquinic acids; 4 hydroxycinnamic acids – caffeic, p-coumaric, ferulic and sinapic acids and 3 flavonol glycosides –...
Tipo: Article Palavras-chave: Dracaena draco leaf; Volatiles; Polyphenols; Organic acids; Haemolysis inhibition; Antioxidant activity.
Ano: 2011 URL: http://hdl.handle.net/10198/3124
Registros recuperados: 23
Primeira ... 12 ... Última
 

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