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Comparison of thin-layer chromatography, spectrofluorimetry and bright greenish-yellow fluorescence test for aflatoxin detection in corn BABT
Ono,Elisabete Yurie Sataque; Silva,Marcelo da; Ribeiro,Ricardo Marcelo Reche; Ono,Mario Augusto; Hayashi,Luciana; Garcia,Glauco Tironi; Hirooka,Elisa Yoko.
In this study the bright greenish-yellow fluorescence test, widely used by the corn milling industry, was compared to the thin-layer chromatography (TLC) and spectrofluorimetry methods for aflatoxin detection in 40 corn samples naturally contaminated by the Aspergillus section Flavi. According to the corn processing industry criteria, all the samples were adequate for human and animal consumption by the bright greenish-yellow fluorescence test, but TLC and spectrofluorimetry analysis detected aflatoxins above the maximum tolerated limit (20 µg/kg) in 7 and 8 samples, respectively. Aflatoxins were detected in 16 (40%) corn samples by TLC, with levels ranging from 4.0 to 54.0 µg/kg (mean 19.97 ± 15.97 µg/kg), and in 25 (62.5%) samples by spectrofluorimetry,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thin-Layer Chromatography; Spectrofluorimetry; BGYF; Aflatoxins; Corn; Mycotoxins.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300025
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Fumonisins in corn: correlation with Fusarium sp. count, damaged kernels, protein and lipid content BABT
Ono,Elisabete Yurie Sataque; Biazon,Luciana; Silva,Marcelo da; Vizoni,Édio; Sugiura,Yoshitsugu; Ueno,Yoshio; Hirooka,Elisa Yoko.
Natural fungal and fumonisin contamination were evaluated in 109 freshly harvested corn samples from Paraná State and correlated to damaged kernels (%). In addition, healthy and damaged kernels of 24 corn samples were selected in order to compare the mycoflora profile and fumonisin levels. The correlation among protein/lipid content and fumonisin levels was also analyzed in the 15 most frequently cultivated corn hybrids. Total fungal colony count in 109 freshly harvested corn samples ranged from 1.9x10(4) to 3.5x10(6) CFU/g, Fusarium sp. count from 1.0x10³ to 2.2x10(6) CFU/g, and fumonisin levels from 0.13 to 20.38 µg/g. Total fungal colony/Fusarium sp. count and fumonisin levels showed positive correlation (p < 0.05). In addition, there was a positive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoflora; Toxigenic fungi; Mycotoxins; Fumonisins; Corn quality.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100008
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Nest and egg description of threatened Herpsilochmus spp. from coastal forest habitats in Rio Grande do Norte, Brazil (Aves: Thamnophilidae) Zoologia
Silva,Marcelo da; Pichorim,Mauro; Cardoso,Márcio Z..
We provide description of eggs and nest structure for two Brazilian antwrens, including the IUCN "vulnerable" species, H. pectoralis Sclater, 1857, and the BirdLife International "near-threatened", H. sellowi Whitney &amp; Pacheco, 2000. Our observations derive from a larger study on Herpsilochmus Cabanis, 1847 distribution in coastal forest fragments in the northern range limit of the Brazilian Atlantic rainforest. Both species nested in relatively open forest. The nest of H. sellowi was built with fungal hyphae, a trait common among some birds. Both eggs were moderately beige with brown spots. Most of the information on Brazilian antwren nesting comes from species that do not occur in the Atlantic rainforest. This is the first account for nest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Herpsilochmus pectoralis; Herpsilochmus sellowi.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752008000300027
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