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| Aubourg, S.; Martin-Magniette, M.L.; Brunaud, V.; Taconnat, L.; Bitton, F.; Balzergue, S.; Jullien, P.E.; Ingouff, M.; Thareau, V.; Schiex, T.; Lecharny, A.; Renou, J.P.. |
| Background: Since the finishing of the sequencing of the Arabidopsis thaliana genome, the Arabidopsis community and the annotator centers have been working on the improvement of gene annotation at the structural and functional levels. In this context, we have used the large CATMA resource on the Arabidopsis transcriptome to search for genes missed by different annotation processes. Probes on the CATMA microarrays are specific gene sequence tags (GSTs) based on the CDS models predicted by the Eugene software. Among the 24 576 CATMA v2 GSTs, 677 are in regions considered as intergenic by the TAIR annotation. We analyzed the cognate transcriptome data in the CATMA resource and carried out data-mining to characterize novel genes and improve gene models.... |
| Tipo: Journal Article |
Palavras-chave: ADNC; EXPRESSION DES GENES; SEQUENCE NUCLEOTIDIQUE; ARABIDOPSIS THALIANA; GENOME; MODELE; METHODE STATISTIQUE; PROTEINE; TRANSCRIPTION; ARN MESSAGER; PCR; RT-PCR CDNA MICROARRAY DATA; EXPRESSION PROFILES; SEQUENCE TAGS; ANNOTATION; THALIANA; NORMALIZATION; REGIONS; TOOLS. |
| Ano: 2007 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20116115e9a4&uri=/notices/prodinra1/2011/03/ |
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| Gagnot, S.; Tamby, J.P.; Martin-Magniette, M.L.; Bitton, F.; Taconnat, L.; Balzergue, S.; Aubourg, S.; Renou, J.P.; Lecharny, A.; Brunaud, V.. |
| CATdb is a free resource available at http://urgv.evry.inra.fr/CATdb that provides public access to a large collection of transcriptome data for Arabidopsis thaliana produced by a single Complete Arabidopsis Transcriptome Micro Array (CATMA) platform. CATMA probes consist of gene-specific sequence tags (GSTs) of 150500 bp. The v2 version of CATMA contains 24 576 GST probes representing most of the predicted A. thaliana genes, and 615 probes tiling the chloroplastic and mitochondrial genomes. Data in CATdb are entirely processed with the same standardized protocol, from microarray printing to data analyses. CATdb contains the results of 53 projects including 1724 hybridized samples distributed between 13 different organs, 49 different developmental... |
| Tipo: Journal Article |
Palavras-chave: CDNA MICROARRAY DATA; SEQUENCE TAGS; INFORMATION; GENOME; NORMALIZATION; BIOLOGY; GENE COMPONENTS ; ADNC; SEQUENCE NUCLEOTIDIQUE; NORMALISATION; BIOLOGIE; ARABIDOPSIS THALIANA; MUTATION; MITOCHONDRIE; CHLOROPLASTE; METHODE STATISTIQUE; EXPRESSION DES GENES. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20111a416d88&uri=/notices/prodinra1/2011/03/ |
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| Herbette, S.; Taconnat, L.; Hugouvieux, V.; Piette, L.; Magniette, M.L.; Cuine, S.; Auroy, P.; Richaud, P.; Forestier, C.; Bourguignon, J.; Renou, J.P.; Vavasseur, A.; Leonhardt, N.. |
| Transcriptional regulation in response to cadmium treatment was investigated in both roots and leaves of Arabidopsis, using the whole genome CATMA microarray containing at least 24,576 independent probe sets. Arabidopsis plants were hydroponically treated with low (5 mu M) or high (50 mu M) cadmium concentrations during 2, 6, and 30 hours. At each time point, Cd level was determined using ICP-AES showing that both plant tissues are able to accumulate the heavy metal. RT-PCR of eight randomly selected genes confirmed the reliability of our microarray results. Analyses of response profiles demonstrate the existence of a regulatory network that differentially modulates gene expression in a tissue- and kinetic-specific manner in response to cadmium. One of the... |
| Tipo: Journal Article |
Palavras-chave: TRANSCRIPTION; METABOLISME SOUFRE MICROARRAY; SULFUR METABOLISM. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007c9bee07b&uri=/notices/prodinra1/2008/08/ |
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