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Registros recuperados: 22
Primeira ... 12 ... Última
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A study of ion-exchange chromatography in an expanded bed for bovine albumin recovery BABT
Severo Jr.,João Batista; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
In the present work, the effect of bed expansion on BSA adsorption on Amberlite IRA 410 ion-exchange resin was studied. The hydrodynamic behavior of an expanded bed adsorption column on effects of the biomolecules and salt addition and temperature were studied to optimize the conditions for BSA recovery on ion-exchange resin. Residence time distribution showed that HEPT, axial dispersion and the Pecletl number increased with temperature and bed height, bed voidage and linear velocity. The binding capacity of the resin increased with bed height. The Amberlite IRA 410 ion-exchange showed an affinity for BSA with a recovery yield of 78.36 % of total protein.
Tipo: Info:eu-repo/semantics/article Palavras-chave: BSA; Protein adsorption; Breakthrough curves; Amberlite IRA 410 ion-exchange resin; Distribution residence time study; Richardson-Zack coefficient.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200021
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Avaliação da lipase extracelular de Pseudomonas cepacia para purificação em sistema bifásico aquoso Ciênc. Tecnol. Aliment.
Padilha,Giovana Silva; Ferreira,Juliana Ferrari; Castiglioni,Gabriel Luis; Alegre,Ranulfo Monte; Tambourgi,Elias Basile.
O presente trabalho teve como objetivo a produção de lipase a partir de Pseudomona cepacia por fermentação líquida em biorreator do tipo Bioflo III. As fermentações foram conduzidas a 150 rpm durante 96 horas a 30 °C. Analisou-se a atividade enzimática em diferentes condições de temperatura (20 a 50 °C) e pH (3,0 a 11,0), e obtiveram-se 37 °C e 8,0, as condições ótimas, respectivamente. Para avaliar a estabilidade térmica, a enzima foi incubada em temperaturas de 40, 50 e 60 °C durante 120 minutos. Em uma segunda etapa, foram realizados experimentos preliminares para verificar as condições adequadas de partição da enzima, bem como sua estabilidade e condições ótimas de hidrólise frente às modificações de temperatura e pH. Foram preparadas soluções de PEG...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas cepacia; Lípase; Purificação.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000100003
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Batch and continuous extraction of bromelain enzyme by reversed micelles BABT
Fileti,Ana Maria Frattini; Fischer,Gilvan Anderson; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reversed micelles; Liquid-liquid extraction; Bromelain; Pineapple; Optimization; Surface response.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500021
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Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp BABT
Lopes,Francisco Luiz Gumes; Severo Júnior,João Baptista; Souza,Roberto Rodrigues de; Ehrhardt,Daniela Diniz; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
The concentration of pineapple juice is needed to retain the bromelain activity and to standardize the composition and proteolytic activity. Thus, this work aimed to obtain a pure bromelain extract from the Ananas comosus L. Merril juice by membrane separation process. A 2² experimental planning was used to study the influence of pH and transmembrane pressure on the activity recovery by micro-filtration using a plain membrane. In second step, this enzyme was purified by the ultra-filtration using a 10 kDa millipore kit. The best operation condition to bromelain concentration using the plain membrane was at pH 7.5 and transmembrane pressure of 0.05 bar, while 85% of bromelain activity was recovered. Ultra-filtration retained 100% of proteolytic activity and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ananas comosus; Bromelain; Membrane separation processes; Purification; Medicinal product.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200024
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Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation BABT
Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de.
This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002
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Extração de corantes de milho (Zea mays L.) Ciênc. Tecnol. Aliment.
Gamarra,Felix Martin Cabajal; Leme,Gisele Costa; Tambourgi,Elias Basile; Bittencourt,Edison.
Os corantes naturais foram amplamente utilizados de forma artesanal até antes do surgimento dos corantes sintéticos. O estudo e uso dos corantes naturais voltaram a ter importância nestes últimos anos devido aos questionamentos dos organismos internacionais da saúde e dos consumidores pelo uso indiscriminado dos corantes sintéticos, ligados ao desenvolvimento de doenças degenerativas e ao impacto ambiental. O corante extraído do milho roxo (Zea mays L.) tem sido utilizado ao longo da história pela civilização Inca na preparação de alimentos e no tingimento de fibras têxteis. Neste trabalho, os pigmentos do grupo das antocianinas foram extraídos das variedades de milho roxo e do milho vermelho (Z. mays L.) e depois foram caracterizados. Três métodos de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antocianina; Corante natural; Milhos roxo e vermelho; Métodos de extração; PH.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612009000100010
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Extraction of lipase from Burkholderia cepacia by PEG/Phosphate ATPS and its biochemical characterization BABT
Padilha,Giovana da Silva; Santana,José Carlos Curvelo; Alegre,Ranulfo Monte; Tambourgi,Elias Basile.
This work aimed to study the partitioning of a lipase produced by Burkholderia cepacia in PEG/Phosphate aqueous two phase system (ATPS) and its characterization. Lipase was produced by B. cepacia strains in a fermenter. Enzyme partitioning occurred at pH 6.0 and 8.0, using PEG 1500 and 6000 on two tie lines. Metal ions, pH and temperature effects on enzyme activity were evaluated. Five milliliter of 7.5% olive oil emulsion with 2.5% gumarabic in 0.1M sodium phosphate buffer at pH 8.0 and 37ºC were used for the activity determinations. Results showed that crude stratum from B. cepacia was partitioned by PEG1500/phosphate ATPS at pH 6.0 or 8.0 for, which the partitioning coefficients were 108-and 209-folds. Lipase presented optimal activity conditions at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Burkholderia cepacia; Aqueous two phase system; Partitioning; Characterization.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100002
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Imobilização de enzimas de milho maltado em gel Ciênc. Tecnol. Aliment.
Silva,Rosa Luísa de Farias Oliveira Bezerra e; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
Este trabalho objetivou a otimização da imobilização das enzimas amilases extraídas do malte do milho, usando alginato de sódio. A concentração do malte no extrato, a porcentagem de alginato de sódio e o pH foram usados como fatores que influenciam na imobilização das enzimas. Os resultados mostraram que as melhores condições de imobilização foram obtidas quando se usou as soluções de malte de milho em duas faixas de concentrações, uma entre 3,75 a 5 g.L-1 e outra entre 15 a 16,25 g.L-1, em pH entre 4,83 a 6,6 e 4% (m/v) de alginato de sódio, condições nas quais se conseguiu imobilizar 100% das enzimas com baixa perda de atividade. Este trabalho mostrou como se obter amilases de malte de milho imobilizadas por oclusão em alginato de sódio e que podem ser...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Imobilização; Amilases; Alginato de sódio; Malte de milho; Oclusão; Biocatalisador.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612008000300021
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Isolation and purification of bromelain from waste peel of pineapple for therapeutic application BABT
Bresolin,Iara Rocha Antunes Pereira; Bresolin,Igor Tadeu Lazzarotto; Silveira,Edgar; Tambourgi,Elias Basile; Mazzola,Priscila Gava.
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromelain; Pineapple peel; Downstream processing; Waste; Therapeutic applications; Enzymatic activity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012
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Microalgae: Cultivation Aspects and Bioactive Compounds BABT
Coêlho,Diego de Freitas; Tundisi,Louise Lacalendola; Cerqueira,Keilla Santos; Rodrigues,Jacqueline Rego da Silva; Mazzola,Priscila Gava; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de.
Abstract Microalgae are aquatic unicellular microorganisms that can be found both in freshwater and marine systems; are capable of photosynthesis; and can grow as individual cells or associated in chains or small colonies. Microalgae cultivation has gained large momentum among researchers in the past decades due to their ability to produce value metabolites, remarkable photosynthetic efficiency, and versatile nature. The wide technological potential, and thus increasing amount of scattered knowledge, may become the very first barrier that a post graduating student, or any non-specialist reader, will face when introduced to the subject. In this review paper, we access the core aspects of microalgae technology, covering their main characteristics, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microalgae; Microalgae Selection and Isolation; Green Products; Microalgae Cultivation; Bioactive Compounds.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000101000
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Neural modeling of bromelain extraction by reversed micelles BABT
Fileti,Ana Maria Frattini; Fischer,Gilvan Anderson; Tambourgi,Elias Basile.
A pulsed-cap microcolumn was used for bromelain extraction from pineapple juice by reversed micelles. The cationic micellar solution used BDBAC as the surfactant, isooctane as the solvent and hexanol as the co-solvent. In order to capture the dynamic behavior and the nonlinearities of the column, the operating conditions were modified in accordance with the central composite design for the experiment, using the ratio between the light phase flow rate and the total flow rate, and the time interval between pulses. The effects on the purification factor and on total protein yield were modeled via neural networks. The best topology was defined as 16-9-2, and the input layer was a moving window of the independent variables. The neural model successfully...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromelain; Reversed micelles; Extraction; Neural networks; Pineapple.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200026
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Otimização da produção de álcool de mandioca Ciênc. Tecnol. Aliment.
Curvelo-Santana,José Carlos; Ehrhardt,Daniela Diniz; Tambourgi,Elias Basile.
Este trabalho objetivou otimizar o processo de hidrólise do amido de mandioca com α-amilase de A. niger e obter o álcool deste xarope. Os ensaios foram realizados a pH 4,8; em que variaram-se a concentração do amido (entre 7-22 g.L-1) e a temperatura (entre 30-59,1 ºC). Durante a fermentação, usaram-se nos mostos 2,2 e 5% de amido de mandioca. Os resultados da hidrólise mostraram que o tempo ficou entre 20-200 minutos; a análise RSM mostrou que o rendimento diminuiu nas concentrações médias; e as condições ótimas foram encontradas entre 55-59,1 ºC e com a concentração entre 7,9-10 ou 20-22 g.L-1, em que se hidrolisou 80% do amido. A melhor condição de fermentação foi obtida para o mosto contendo 5% de amido. Sua composição final foi de 0,668 g.L-1 de ART,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amido; Hidrólise; Manihot sp.; Amilases; Álcool; Otimização RSM.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000300007
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Otimização da secagem do malte de Zea mays Ciênc. Tecnol. Aliment.
Biazus,Joana Paula Menezes; Souza,Roberto Rodrigues de; Curvelo-Santana,José Carlos; Tambourgi,Elias Basile.
Neste trabalho foi feita a otimização do processo de secagem do malte de milho (Zea mays) com o objetivo de gerar um derivado de alto valor comercial sem reduzir a atividade das enzimas presentes neste, alfa e beta - amilases, e tentar agregar valor à cultura do milho. Modelos de curvas de secagem foram usados para simular as isotermas de secagem. Um planejamento fatorial completo do tipo 2² foi feito, usando como fatores o tempo (5,18; 6; 8; 10 e 10,8 h) em torno do tempo de secagem para determinação de umidade em alimento e a temperatura (54, 65 e 76 °C) próxima do ótimo das enzimas e a atividade enzimática no malte como resposta. O modelo que mais se ajustou aos dados de cinética de secagem foi o de Henderson e Henderson, e a umidade média retirada do...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzimas alfa e beta-amilases; Malte de milho (Zea mays); Secagem; Otimização.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612006000400012
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PARTIAL CHARACTERIZATION OF PROTEASES FROM STREPTOMYCES CLAVULIGERUS USING AN INEXPENSIVE MEDIUM BJM
Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Duarte,Helena Simões; Tambourgi,Elias Basile; Melo,Eduardo Henrique Magalhães de; Silva,Valdinete Lins; Porto,Ana Lúcia Figueiredo; Lima Filho,José Luiz de.
The partial characterization of extracellular proteases from Streptomyces clavuligerus NRRL 3585 and 644 mutant was investigated. The enzyme production was carried out in batch fermentation using soy bean filtrate as nitrogen source. Maximum activity was obtained after 96h of fermentation with an initial pH of 7.0. The enzyme was partially purified by ammonium sulphate precipitation. Enzymes from the two strains retained 37% of their initial activities at pH 8.0 after 2 h incubation at 25ºC. Enzyme half-life at pH 8.0 and 60ºC was 40.30 and 53.32 min, respectively for both strains (partially purified extract). The optimum pH was obtained at pH 7.0-8.0 and 8.4 for enzymes produced for 3585 and 644 strains (crude extract), respectively, and 8.4 and 8.0 for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces clavuligerus; Extracellular proteases; Thermal stability; PH.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300010
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Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol) BABT
Sarubbo,Leonie Asfora; Oliveira,Luciana Alves de; Porto,Ana Lúcia Figueiredo; Campos-Takaki,Galba Maria de; Tambourgi,Elias Basile.
The partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several parameters including concentrations of polymers, pH, salt addition and temperature on the partitioning of these proteins were investigated.. The results of this research demonstrated the importance of the protein characteristics for partitioning in aqueous biphasic system.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aqueous two-phase systems; Ine serum albumin; Cashew-nut tree gum; Protein partition.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500003
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Partitioning optimization of proteins from Zea mays malt in ATPS PEG 6000/CaCl2 BABT
Ferreira,Graziela Batista; Evangelista,Alex Ferreira; Severo Junior,João Baptista; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile; Jordão,Elizabete.
This work aimed to establish the relationship between the compositions and pH of ATPS PEG 6000/CaCl2 and the proteins partition from maize malt and also to simplify the process optimization in ATPS for a statistical model, established by response surface methodology (RSM). Results showed that these were no influence of pH on the phase diagrams and on the composition of tie line length of PEG 6000/CaCl2 ATPS. SRM analyses showed that elevated pH and larger tie line length were the best conditions for recovering of maize malt proteins. The maximum partition coefficient by PEG 6000/CaCl2 ATPS was about 4.2 and was achieved in ATPS in a single purification step. The theoretical maximum partition coefficient was between 4.1-4.3. The process was very suitable...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Partitioning; Optimization; Aqueous two-phase systems; Maize malt; PEG 6000; CaCl2.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300020
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Production and characterization of amylases from Zea mays malt BABT
Biazus,Joana Paula Menezes; Souza,Roberto Rodrigues de; Márquez,Jesus Espinoza; Franco,Telma Teixeira; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
In this work the α and β-amylase enzymes were obtained from maize (Zea mays) malt and were biochemistry characterized. A germination study to obtain the maize malt with good amylase activity was made. The maize seeds were germinated in laboratory and the enzymatic activity was measured daily. Activity dependence to germination time were fitted to an exponential model (A=A0eµt), which showed that the behaviour of enzymatic activity in the germination process was similar to the growth of the microorganism. Its model could be applied to describe the mechanism of α-amylase production for each maize varieties and others cereals. Maize malt characterization showed that α and β-amylase had optimal pH between 4-6.5, optimal temperature 50 and 90ºC, and molecular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Maize seed; Malting; Α and β-amylase; Biochemistry characterization; Germination model.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400024
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Purificação de amilases de malte de Zea mays Ciênc. Tecnol. Aliment.
Biazus,Joana Paula Menezes; Santana,José Carlos Curvelo; Souza,Roberto Rodrigues de; Tambourgi,Elias Basile.
Neste trabalho realizou-se a purificação das enzimas α e β-amilases de malte de milho combinando os processos de adsorção em leito expandido com o fracionamento por etanol. Alimentaram-se 5 mL de uma solução a 2% de malte de milho em tampão fosfato 0,05 M a pH 5 em uma coluna contendo as resinas de troca iônica Amberlite IRA 410, em leito fixo de 4 cm fluidizado para 8 cm. A eluição foi promovida com NaCl 0,25M com 14 mL/minuto de vazão descendente e o material eluído foi fracionado com álcool etílico nas concentrações de 40-70%, 50-80% e 60-90% (v/v) a 20 e 30 ºC. A recuperação da atividade e o fator de purificação foram de 95% e 23 vezes, respectivamente, na primeira etapa. A melhor condição de fracionamento das enzimas foi apresentada a 50-80% de álcool...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adsorção em leito expandido; Precipitação; Amilases; Malte de milho; Álcool etílico; Resina de troca iônica.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000100032
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Recovery of ascorbic oxidoreductase from crude extract with an aqueous two-phase system in a perforated rotating disc contactor BABT
Porto,Ana Lúcia Figueiredo; Sarubbo,Leonie Asfora; Moreira,Keila Aparecida; Melo,Homero José Farias de; Lima-Filho,José Luís; Campos-Takaki,Galba Maria; Tambourgi,Elias Basile.
A continuous perforated rotating disc contactor was used to extract the enzyme ascorbic oxidoreductase (E.C.1.10.3.3) from crude extract of Curcubita maxima with an aqueous two-phase system of poly (ethylene glycol) and phosphate salts. The effect of dispersed phase velocity on either protein mass transfer coefficients or separation efficiency at 1, 2 and 3 mL/min was studied. An increase of the mass transfer coefficients was observed with the dispersed phase velocity, while the separation efficiency showed a small decrease with the increase of this parameter. The experimental results obtained during continuous extraction showed that the ascorbic oxidoreductase activity was partitioned preferentially into the salt-rich phase in all conditions studied. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Efficiency; Mass transfer coefficient; Perforated Rotating Disc contactor; Aqueous two-phase system; Ascorbic oxidoreductase.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500019
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Response surface methodology to evaluation the recovery of amylases by hollow fiber membrane BABT
Severo Júnior,João Baptista; Oliveira,Laura Sampaio de Sá; Sardeiro,Fernanda Silva; Souza,Roberto Rodrigues de; Lopes,Francisco Luiz Gumes; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
This work aimed to study the pH and the transmembrane pressure effects during the recovery of alpha and beta amylases enzymes from corn malt (Zea mays) by hollow fiber membrane. The optimal condition was obtained for a statistical model, established by response surface methodology (RSM). The response surface analysis showed that the best operation condition for amylolitics enzymes recovery by hollow fiber membrane was 0.05 bar and pH 5.00, while the enzymes were purified about of 26 times.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hollow fiber membrane; Corn malt; Alpha and beta amylase; RSM.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400017
Registros recuperados: 22
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