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Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae) Genet. Mol. Biol.
Lopes,Denilce Meneses; Campos,Lúcio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melipona; Stingless bees; Microsatellite; Transferability.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200030
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Cytogenetic characterization of Melipona rufiventris Lepeletier 1836 and Melipona mondury Smith 1863 (Hymenoptera, Apidae) by C banding and fluorochromes staining Genet. Mol. Biol.
Lopes,Denilce Meneses; Pompolo,Sílvia das Graças; Campos,Lúcio Antônio de Oliveira; Tavares,Mara Garcia.
The stingless bees Melipona rufiventris and M. mondury were analyzed cytogenetically by conventional staining with Giemsa, C-banding and sequential staining with the fluorochromes CMA3/DA/DAPI. Both species presented 2n = 18 and n = 9, except for one colony of M. rufiventris, in which some individuals had 2n = 19 due to the presence of a B chromosome. After Giemsa staining and C-banding the chromosomes appeared very condensed and presented a high heterochromatic content, making it difficult to localize the centromere and therefore to visualize the chromosomes morphology. The constitutive heterochromatin was located in interstitial chromosome regions covering most of the chromosomes extension and consisted mainly of AT, as shown by DAPI staining. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melipona; Stingless bees; B chromosomes; Fluorochromes; C-banding.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100010
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Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes Genet. Mol. Biol.
Marthe,Jefferson de Brito; Pompolo,Silvia das Graças; Campos,Lucio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Four colonies of the stingless bee Partamona cupira (Hymenoptera: Apidae) were cytogenetically analyzed using conventional staining and the fluorochromes CMA3 e DAPI. The females have 2n = 34 chromosomes (2K=32<img border=0 src="../../../../../../../img/revistas/gmb/2010nahead/ah06a04CarM.gif" align=absmiddle>+2<img border=0 src="../../../../../../../img/revistas/gmb/2010nahead/ah06a04CarA.gif" align=absmiddle>). Some females, however, presented an additional large B acrocentric chromosome, to a total of 2n = 35. Chromosome B and the chromosomal pairs 2, 9 and 10 showed CMA3+ bands, indicating an excess of CG base-pairs. A clear association was verified between the P. helleri B chromosome SCAR marker and the presence of a B chromosome in P....
Tipo: Info:eu-repo/semantics/article Palavras-chave: B chromosome; SCAR; Stingless bees.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200009
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Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae) Genet. Mol. Biol.
Tavares,Mara Garcia; Carvalho,Carlos Roberto; Soares,Fernanda Aparecida Ferrari; Fernandes,Anderson.
When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetic; Flow cytometry; Genome size; Karyotype; Stingless bee.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300019
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Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations Genet. Mol. Biol.
Tavares,Mara Garcia; Pietrani,Nathalia Teixeira; Durvale,Maxwell de Castro; Resende,Helder Canto; Campos,Lucio Antonio de Oliveira.
Melipona quadrifasciata is a stingless bee widely found throughout the Brazilian territory, with two recognized subspecies, M. quadrifasciata anthidioides, that exhibits interrupted metasomal stripes, and M. quadrifasciata quadrifasciata, with continuous metasomal stripes. This study aimed to estimate the genetic variability of these subspecies. For this purpose, 127 colonies from 15 Brazilian localities were analyzed, using nine species-specific microsatellite primers. At these loci, the number of alleles ranged from three to 15 (mean: 7.2), and the observed heterozygosity (Ho) ranged from 0.03-0.21, while the expected heterozygosity (He) ranged from 0.23-0.47. The genetic distances among populations ranged from 0.03-0.45. The F ST multilocus value (0.23)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic differentiation; Microsatellites; Population genetics; Stingless bees.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016
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Genetic divergence between populations of the stingless bee uruçu amarela (Melipona rufiventris group, Hymenoptera, Meliponini): is there a new Melipona species in the Brazilian state of Minas Gerais? Genet. Mol. Biol.
Tavares,Mara Garcia; Dias,Luiz Antonio dos Santos; Borges,Andréia Arantes; Lopes,Denilce Meneses; Busse,Angélica Helene Pereira; Costa,Ronaldo Guimarães; Salomão,Tânia Maria Fernandes; Campos,Lucio Antonio de Oliveira.
Allozyme, microsatellite and random amplified polymorphic DNA (RAPD) molecular markers were used to investigate the within and between population genetic variability and between population genetic differentiation of the Brazilian stingless bee uruçu amarela (nominally Melipona rufiventris Lepeletier, 1836) present in savanna and Atlantic forest habitats of the Brazilian state of Minas Gerais (MG). We found low levels of within population variability, although there were a large number of private alleles that specifically characterized these populations. The F ST values indicated a high level of genetic diversity between populations. Analysis of molecular variance (AMOVA) showed a high degree of population differentiation between the savanna and Atlantic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endangered species; Genetic divergence; Molecular markers; Stingless bees; Uruçu amarela.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400027
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Genetic diversity and structure of Atta robusta (Hymenoptera, Formicidae, Attini), an endangered species endemic to the restinga ecoregion Genet. Mol. Biol.
Reis,Evelyze Pinheiro dos; Salomão,Tânia Maria Fernandes; Campos,Lucio Antonio de Oliveira; Tavares,Mara Garcia.
The genetic diversity and structure of the ant Atta robusta were assessed by ISSR (inter-simple sequence repeats) in 72 colonies collected from 10 localities in the Brazilian states of Espírito Santo (48 colonies) and Rio de Janeiro (24 colonies). The ISSR pattern included 67 bands, 51 of them (76.1%) polymorphic. Analysis of molecular variance (AMOVA) revealed a high level (57.4%) of inter-population variation, which suggested a high degree of genetic structure that was confirmed by UPGMA (unweighted pair-group method using an arithmetic average) cluster analysis. The significant correlation between genetic and geographic distances (r = 0.64, p < 0.05) indicated isolation that reflected the distance between locations. Overall, the populations were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endemism; Endangered; ISSR; Population genetics; Restinga.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400015
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Genetic variability in five populations of Partamona helleri (Hymenoptera, Apidae) from Minas Gerais State, Brazil Genet. Mol. Biol.
Borges,Andreia Arantes; Campos,Lucio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Partamona is a Neotropical genus of stingless bees that comprises 33 species distributed from Mexico to southern Brazil. These bees are well-adapted to anthropic environments and build their nests in several substrates. In this study, 66 colonies of Partamona helleri from five localities in the Brazilian state of Minas Gerais (São Miguel do Anta, Teixeiras, Porto Firme, Viçosa and Rio Vermelho) were analyzed using nine microsatellite loci in order to assess their genetic variability. Low levels of observed (Ho = 0.099-0.137) and expected (He = 0.128-0.145) heterozygosity were encountered and revealed discrete genetic differentiation among the populations (F ST =0.025). AMOVA further showed that most of the total genetic variation (94.24%) in P. helleri was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Partamona helleri; Population genetics; Stingless bees.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400028
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Prediction of social structure and genetic relatedness in colonies of the facultative polygynous stingless bee Melipona bicolor (Hymenoptera, Apidae) Genet. Mol. Biol.
Reis,Evelyze Pinheiro dos; Campos,Lucio Antonio de Oliveira; Tavares,Mara Garcia.
Stingless bee colonies typically consist of one single-mated mother queen and her worker offspring. The stingless bee Melipona bicolor (Hymenoptera: Apidae) shows facultative polygyny, which makes this species particularly suitable for testing theoretical expectations concerning social behavior. In this study, we investigated the social structure and genetic relatedness among workers from eight natural and six manipulated colonies of M. bicolor over a period of one year. The populations of M. bicolor contained monogynous and polygynous colonies. The estimated genetic relatedness among workers from monogynous and polygynous colonies was 0.75 ± 0.12 and 0.53 ± 0.16 (mean ± SEM), respectively. Although the parental genotypes had significant effects on genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melipona; Microsatellites; Polygyny; Queen number; Social structure.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200028
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Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera) Genet. Mol. Biol.
Tavares,Mara Garcia; Azeredo-Oliveira,Maria Tercilia Vilela de; Ceron,Carlos Roberto.
We examined the esterases present in the hemolymph and Malpighian tubules of "Kissing bug", Triatoma infestans (Triatominae, Heteroptera) by polyacrylamide gel electrophoresis. Six esterase bands were observed and were designated EST 1 to EST 6. EST 1, 4, 5 and 6 were exclusive to hemolymph, whereas EST 2 and 3 were found only in Malpighian tubules. Each tissue had a characteristic esterase pattern, which may be related to its functional role. The four hemolymph esterases hydrolyzed a-naphthyl acetate. One of these enzymes was classified as a carboxylesterase (EST 4), and another was an acetylesterase (EST 6). The other two enzymes (EST 1 and 5) could be either carboxylesterases or serino-proteases with an esterolytic function, as they were selectively...
Tipo: Info:eu-repo/semantics/article
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400009
Registros recuperados: 10
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