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Aspects on the catalysis of lipase from porcine pancreas (type VI-s) in aqueous media: development of ion-pairs BABT
Kokkinou,Marina; Theodorou,Leonidas G.; Papamichael,Emmanuel M..
This article reports a first contribution for the elucidation of catalytic mechanism of Lipase from porcine pancreas, type VI-s (PPL), in hydrolyzing an ester substrate in aqueous media. The conclusions were based on the pH-profiles of Michaelis-Menten parameters k cat/Km, k cat and Km, as well as on the absolute temperature profile of k cat/Km, obtained during the hydrolysis of p-nitrophenyl laurate by PPL. It was found that (a) PPL performs catalysis by means of ion pairs formed either as Ser152-Ο-/His263-Im+H and/or Carbonyl-Ο-/His263-Im+H, (b) the parameter k cat/Km equals to k1 and thus ES is formed and destroyed in the course of a series of consecutive reactions governed by the dynamic constant K S = k2/k1, and (c) the hydrolysis of substrate is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Porcine Pancreas Lipase; Mechanism of hydrolysis; P-nitrophenyl laurate.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200007
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Necessary auxiliary background for efficient use of an existing computer program of non-parametric fitting of nonlinear equations BABT
Papamichael,Emmanuel M.; Theodorou,Leonidas G..
This work constituted a significant contribution for more efficient use of a valuable computer program of non-parametric fitting of nonlinear multiparametric equations to experimental data. However, prerequisite in this context was the transformation of nonlinear multiparametric equations into linear hyperplane forms before their incorporation within the computer program; this latter was decisive and a matter of proper programming practice. Herein, a series of widely used equations useful in different fields of chemical processes, in biochemistry and/or in biotechnology, along with their suitable transformations as well as the appropriate programming support are being reported.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Non-parametric methods; Computer program; Fitting of nonlinear equations.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200022
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