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Azole susceptibility in clinical and environmental isolates of Aspergillus fumigatus from eastern Hokkaido, Japan OAK
Toyotome, Takahito; Fujiwara, Takayuki; Kida, Hideyuki; Matsumoto, Munehiro; Wada, Terumi; Komatsu, Ryoichi.
Azole antifungals are used not only clinically for fungal infections but also used as agricultural fungicides. Recently, azole-resistant Aspergillus fumigatus containing a tandem repeat in the promoter region of cyp51A combined with amino acid substitution(s) appears in the environment in Eurasia, especially in several European countries. Although azole fungicides have been used in Japan, especially in Hokkaido, surveillance and characterization of A. fumigatus in Hokkaido have not been reported. In this study, we collected soil samples from farms that used an azole fungicide in the Tokachi area of eastern Hokkaido, isolated 91 A. fumigatus strains, and determined the minimal inhibitory concentrations of medical azoles required for these strains. Moreover,...
Palavras-chave: Azole; Aspergillus fumigatus; Cyp51A.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4469
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Azole-resistant aspergillus fumigatus containing a 34-bp tandem repeat in cyp51A promoter is isolated from the environment in Japan OAK
Onishi, Kenji; Sarumoh, Bolu Muhammad; Hagiwara, Daisuke; Watanabe, Akira; Kamei, Katsuhiko; Toyotome, Takahito.
Azole-resistant strains of Aspergillus fumigatus containing a tandem repeat in the cyp51A promoter and amino acid substitution(s) have been isolated in the environment worldwide; however, this type of resistant strain had never been isolated from the environment in Japan. Our previous study indicated that an azole-resistant A. fumigatus strain OKH50 containing a 34-bp tandem repeat in cyp51A promoter with L98H substitution in Cyp51A was isolated from a patient in Obihiro of Hokkaido, Japan. In this study, we collected azole-resistant Aspergillus spp. by air sampling from the environment in Japan. One Aspergillus-like colony was isolated from one of 10 sampling sites surveyed. The strain Env1 was confirmed as A. fumigatus by nucleotide sequencing and...
Palavras-chave: Aspergillus fumigatus; Azole; Cyp51A; TR34/L98H.
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4565
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MEIS3 is repressed in A549 lung epithelial cells by deoxynivalenol and the repression contributes to the deleterious effect OAK
Toyotome, Takahito; Takahashi, Hiroki; Kamei, Katsuhiko.
Deoxynivalenol (DON) is an important Fusarium toxin of concern for food safety. The inhalation of powder contaminated with DON is possible and may cause lung toxicity. In this study, we analyzed the gene expression profile of A549 cells treated for 24 hr with 0.2 µg/mL DON by microarray analysis. In total, 16 genes and 5 noncoding RNAs were significantly affected by DON treatment. The repression of B3GALT4, MEIS3, AK7, SEMA3A, KCNMB4, and SCARA5 was confirmed by quantitative PCR. We investigated the DON toxicity on A549 cells that exogenously expressed these 6 genes. The result indicated that A549 cells that transiently expressed MEIS3 were tolerant to the deleterious effects of DON. Our data show that DON affected the expression of genes with various...
Palavras-chave: Fusarium toxin; Mycotoxin; Deoxynivalenol; Trichothecene; Lung epithelial cell.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4301
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