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A naturally occurring mutation K220T in the pleiotropic activator PrfA of Listeria monocytogenes results in a loss of virulence due to decreasing DNA-binding affinity Inra
Velge, P.; Herler, M.; Johansson, J.; Roche, S.; Témoin, S.; Fedorov, A. A.; Gracieux, P.; Almo, S. C.; Goebel, W.; Cossart, P..
The sequencing of prfA, encoding the transcriptional regulator of virulence genes, in 26 low virulence field Listeria monocytogenes strains showed that eight strains exhibited the same single amino-acid substitution: PrfAK220T. These strains exhibited no expression of PrfA-regulated proteins and thus no virulence. This substitution inactivated PrfA, since expression of the PrfAK220T mutant gene in an EGDΔprfA strain did not restore the haemolytic and phosphatidylcholine phospholipase C activities, in contrast to the wild-type prfA gene. The substitution of the lysine at position 220 occurred in the helix αH. However, the data showed that the PrfAK220T protein is dimerized just as well as its wild-type counterpart, but does not bind to PrfA boxes....
Tipo: Journal Article Palavras-chave: LISTERIA MONOCYTOGENES; PROTEINE; FACTEUR DE VIRULENCE; BACTERIE PATHOGENE; ADN; SEQUENCE NUCLEOTIDIQUE; REGULATION; TRANSCRIPTION; VIRULENCE; EXPRESSION DES GENES; ACIDE AMINE; MUTATION; RT-PCR; ELECTROPHORESE; ENZYME; ARN POLYMERASE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009c1895b3c&uri=/notices/prodinra1/2009/11/
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Avirulence of viable but non-culturable Listeria monocytogenes cells demonstrated by in vitro and in vivo models Inra
Cappelier, J.M.; Besnard, V.; Roche, S.; Garrec, N.; Zundel, E.; Velge, P..
The virulence of Viable But Non-Culturable (VBNC) cells of 4 strains of Listeria monocytogenes was investigated in both a human adenocarcinoma cell line (HT-29) and a mouse model. LO 28, ATCC 19115 and CNL 895807 strains of Listeria monocytogenes became VBNC when incubated in microcosm water at 20 °C and Scott A strain at 4 °C. No culturable bacteria were detected in the VBNC state, although 104 active cells/mL were found by the Direct Viable Count (DVC) and CTC-DAPI double staining methods. A comparison of virulence in both human adenocarcinoma cell line HT-29 and the mouse model showed that culturable controls were more virulent than VBNC cells, which appeared to be avirulent regardless of the virulence methods applied. Pathogenicity was tested in each...
Tipo: Journal Article Palavras-chave: VBNC; VIABLE BUT NON CULTURABLE; PLAQUE FORMING ASSAY; VIRULENCE.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007e324a9ea&uri=/notices/prodinra1/2009/12/
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Emergence of Salmonella epidemics: the problems related to Salmonella enterica serotype Enteritidis and multiple antibiotic resistance in other major serotypes Inra
Velge, P.; Cloeckaert, A.; Barrow, P..
Tipo: Journal Article Palavras-chave: EMERGENCE; VIRULENCE; EVOLUTION; SALMONELLA ENTERICA.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0500018099108652&uri=/notices/prodinra1/2009/12/
Registros recuperados: 3
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