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การโคลนนิ่งและหาลำดับเบสของยีนที่ตอบสนองต่อสภาวะแล้งและความสัมพันธ์ระหว่างลักษณะโครงสร้างทางกายวิภาคกับการทนทานต่อสภาพแล้งในข้าวโพด (Zea mays L.) Thai Agricultural
Niran Juntawong; Prasart Kuermanee.
Drought stress could induce expressions of various genes that are involved in stress tolerance and responses. Recent studies were emphasized on a gene encoded for the 9-cis epoxycarotenoid dioxygenase (NCED) enzyme which catalyzes the biosynthesis of abscisic acid (ABA) in plants. However, little is known about drought tolerance genes in maize. We designed primers from the VP14 sequence in the NCBI gene bank for the study of expression of drought tolerance gene in SW 2301 drought tolerant and KSX 4605 drought sensitive lines. The RT-PCR showed an expression of drought responsible gene in SW 2301 drought tolerant line. Under drought condition, the expression of the responsible gene was higher in stem apical meristem than in root and leaf tissues. The cDNA...
Palavras-chave: Zea mays; Cloning; Sequencing; Drought responsible gene; Anatomical structure; Drought tolerance; 9-cis epoxycarotenoid dioxygenase; VP14; NCED; ข้าวโพด; พันธุ์; ยีนทนแล้ง; การโคลนนิ่ง; การหาลำดับเบส; โครงสร้างทางกายวิภาค; การทนแล้ง; กรดแอบไซซิก.
Ano: 2010 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4662
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