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Registros recuperados: 23
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A broad pH range and processive chitinase from a metagenome library BJMBR
Thimoteo,S.S.; Glogauer,A.; Faoro,H.; de Souza,E.M.; Huergo,L.F.; Moerschbacher,B.M.; Pedrosa,F.O..
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene named metachi18A. The metachi18A gene was subcloned and overexpressed in Escherichia coli BL21 and the MetaChi18A chitinase was purified by affinity chromatography as a 6xHis-tagged fusion protein. The MetaChi18A enzyme is a 92-kDa protein with a conserved active site domain of glycosyl hydrolases family 18. It hydrolyses colloidal chitin with an optimum pH of 5 and temperature of 50°C. Moreover, the enzyme...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Metagenomic; Aeromonas; Kinetics.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100602
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A prospective study on Aeromonas in outpatients with diarrhea in the central region of Rio Grande do Sul State BJM
Prediger,Karoline de Campos; Pereira,Renata da Silva; Winckler Neto,Carlos Hugo Del Priore; Santos,Roberto Christ Vianna; Fadel-Picheth,Cyntia Maria Telles; Vizzotto,Bruno Stefanello.
Aeromonas spp. were identified in five (2,7%) of 182 diarrheal stool cultures, A. caviae was predominant, resistant mainly to ampicillin and cephalotin. This is the first study showing the presence of Aeromonas spp. in diarrheal stools of outpatients in the central region of Rio Grande do Sul State, Brazil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Stools; Outpatients; Multi-resistance.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300017
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Aeromonas associated diarrhoeal disease in south Brazil: prevalence, virulence factors and antimicrobial resistance BJM
Guerra,Ivani M.F.; Fadanelli,Raquel; Figueiró,Manuela; Schreiner,Fernando; Delamare,Ana Paula L.; Wollheim,Claudia; Costa,Sérgio Olavo P.; Echeverrigaray,Sergio.
Aeromonas were isolated from 27 (6.6%) of 408 patients admitted with acute gastroenteritis in two hospitals at Rio Grande do Sul, Brazil. Isolates were classified as A. hydrophila (51.8%), A. caviae (40.8%), and A. veronii biotype sobria (7.4%). The highest prevalence of Aeromonas associated infections occurred in lactants and children. Virulence genes (aerA -aerolysin/hemolysin, ahpA -serine-protease, satA - glycerophospholipid-cholesterol acyltransferase, lipA -lipase, and ahyB -elastase) and virulence factors (hemolytic, proteolitic, lipolitic activities, and biofilm formation) were identified in most A. hydrophila and A. veronii biotype sobria isolates, with lower frequencies on A. caviae. All Aeromonas isolates were resistant to ampicillin,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Diarrhea disease; Virulence factors; Antimicrobial resistance.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400011
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Aeromonas no processamento de queijos tipos Minas Frescal e Colonial. Repositório Alice
CERESER, N. D.; ROSSI JÚNIOR, O. D.; MARTINELI, T. M.; SOUZA, V. de; RODRIGUES, L. B.; CARDOZO, M. V..
Resumo: Com o objetivo de estabelecer, durante o processamento do queijo Minas Frescal e Colonial os possíveis pontos de contaminação e a forma de disseminação de bactérias do gênero Aeromonas, foram analisados, quanto à presença do micro-organismo, diferentes produtos e pontos do fluxograma de produção. Para o Queijo Minas Frescal, Aeromonas spp. foram isoladas no leite cru, leite pasteurizado, ambiente de produção e nas mãos dos manipuladores. A. caviae foi a espécie mais frequentemente identificada, sendo também isoladas A. sobria e A. schubertii. Durante o processamento do queijo Colonial, as espécies A. hydrophila, A. caviae, A. sobria, A. veronii e A. jandaei foram isoladas a partir da água, mãos dos manipuladores, utensílios, leite cru e após...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Queijo Minas Frescal; Queijo Colonial; Produção artesanal; Bacteria; Microrganismo; Contaminação bacteriana; Higiene de alimento; Microbiologia de alimento; Consumidor; Análise de risco; Aeromonas; Microbiology; Food contamination; Cheese; Risk analysis.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/973437
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Aeromonas presence in drinking water from collective reservoirs and wells in peri-urban area in Brazil BJM
Razzolini,Maria Tereza Pepe; Günther,Wanda Maria Risso; Martone-Rocha,Solange; Luca,Heloísa Duarte de; Cardoso,Maria Regina Alves.
Aeromonas genus is considered an emerging pathogen and its presence in drinking water supplies is a reason to public health concern. This study investigated the occurrence of Aeromonas in samples from collective reservoirs and wells used as drinking water sources in a peri-urban area. A total of 35 water samples were collected from collective reservoirs and 32 from wells bimonthly, from September 2007 to September 2008. Aeromonas spp determination was carried out using a Multiple-Tube Technique. Samples were inoculated into alkaline peptone water and the superficial film formed was transferred to blood agar plates amended with ampicillin. Typical Aeromonas colonies were submitted to a biochemical screening and then to biochemical tests for species...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Peri-urban area; Water quality.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300020
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Aeromonas spp. and microbial indicators in raw drinking water source BJM
Di Bari,Marisa; Hachich,Elayse M.; Melo,Adalgisa M.J.; Sato,Maria I. Z..
Aeromonas species are autochtonous in the aquatic environment and some of them have been associated with health effects like wound infections, septicemia and diarrhoeal illness. In this study, the occurrence of Aeromonas spp. and microbial indicators in raw drinking water from wells, springs, fountains and mineral waters was evaluated. A total of 126 water samples was analyzed for Aeromonas spp. by the membrane filtration technique using ADA media and by P/A test. Typical colonies of Aeromonas spp. were submitted to biochemical tests for species differentiation. Toxin production was tested using Y-1 mouse adrenal cells. Coliforms and heterotrophic bacteria were enumerated by membrane filtration and pour plate techniques, respectively. P. aeruginosa, C....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Raw drinking water; Microbial indicators; Heat labile toxin.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300025
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An in-house multiplex pcr method to detect of putative virulence factors in Aeromonas species BJM
Aguilera-Arreola,Ma. Guadalupe; Martínez,Alma Aidee Carmona; Castro-Escarpulli,Graciela.
A pentaplex PCR was developed and optimised to detect the genes that encode the five most important putative virulence factors in Aeromonas isolates. It seems to be more efficient than previously reported techniques and promises to be a powerful tool for more accurate risk assessments and for monitoring pathogenic strains.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pentaplex-PCR; Virulence potential; Pathogenesis; Aeromonas.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400011
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Antibacterianos de actinomicetos marinhos contra o patógeno oportunista Aeromonas spp. Repositório Alice
SCHINKE, C.; MARTINS, T.; SILVA, F. S. P. da; SOUZA, D. T.; MELO, I. S. de; REYES, F. G. R..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Antimicrobiano; Actinomiceto marinho; Actinomiceto; Aeromonas.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1036513
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Antimicrobial susceptibility, hemolysis, and hemagglutination among Aeromonas spp. isolated from water of a bovine abattoir BJM
Bizani,Delmar; Brandelli,Adriano.
The presence of Aeromonas spp. in food has been demonstrated. They are often introduced from water, which is the natural habitat of many species and is thought to be the main source of contamination. The occurrence of Aeromonas spp. was investigated in 70 water samples of a bovine abattoir. Aeromonas spp. were present in 21.4% of water samples. A. hydrophila was isolated from 11.4% of supplying water samples and from 25.7% of the water drained from carcasses, whereas A. sobria was sole isolated from 5.7% of supplying water samples. Although greater number of positive samples were from water drained from carcasses, only A. hydrophila isolates were present. This suggests that the supplying water is the source of contamination. The antibiotic susceptibility...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Antibiotic activity; Aquatic environment; Hemolysis; Water.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400016
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Bacterial Community Associated with Fish and Water from Congonhas River, Sertaneja, Paraná, Brazil BABT
Sousa,José Américo de; Silva-Souza,Ângela Teresa.
A bacteriological study was conducted on fish and water from Congonhas River, Sertaneja (22º58’ S; 50º58’ W), Paraná State, Brazil. From 44% of the analysed fish, bacteria belonging to Pseudomonas, Acinetobacter, Aeromonas, Enterobacteriaceae, Micrococcus, Bacillus and Lactobacillus were isolated. The group most frequently isolated from fish was Aeromonas. In the water, the bacterial groups detected were Pseudomonas, Acinetobacter, Aeromonas, Enterobacteriaceae, Bacillus and Flavobacterium, from which Flavobacterium and Acinetobacter were the most abundant. The numbers of Colony Forming Units per millilitre of water varied from 3.1x10² to 1.0 x 10³. Although a clear pattern was not detected in the susceptibilities/resistances of the isolated strains to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial community; Fish; Congonhas River; Aeromonas; Pseudomonas; Resistance.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400007
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Bacteries associees aux production de Brachionus plicatilis ArchiMer
Nicolas, Jean-louis; Joubert, M.
Bacteria associated with semi-continuous culture of Brachionus plicatilis , which is used as the first food of marine fish larvae, were counted on Zobell 2216 E medium and identified. The numbers of bacteria were very high in both Brachionus (about 10 super(11) bact. per ml of digestive content) and in water (from 1.6.10 super(6) to 4.7.10 super(7) bact. per ml). The cultures were studied at driving a time when mortalities occurred frequently. The introduction of a Pseudomonas) phenon at a proportion of about 50% to the dominant microflora persisted in time and was little influenced by diet. It has been the cause of the repression of the Vibrio, Aeromonas group which was found in an unusually small proportion. It induced a lack of microflora diversity and...
Tipo: Text Palavras-chave: Brachionus plicatilis; Rotifera; Pseudomonas; Aeromonas; Vibrio; Nutrition; Pathology; Fish culture; Fish larvae; Food organisms; Bacteria; Continuous culture; Ecological associations; Zooplankton culture.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-994.pdf
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Bacterioses. Repositório Alice
NOGUEIRA, M. F.; CRUZ, T. F. da..
2007
Tipo: Capítulo em livro científico (ALICE) Palavras-chave: Brucelose; Leptospirose; Febre maculosa brasileira; Tuberculose bovina; Coto ou papeira; Bacterioses causadas por Enterobacteriaceae; Aeromonas; Campylobacter.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/812596
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Characterization of Aeromonas species isolated from an estuarine environment BJM
Evangelista-Barreto,Norma Suely; Carvalho,Fatima Cristiane Teles de; Vieira,Regine Helena S. dos Fernandes; dos Reis,Cristhiane Moura Falavina; Macrae,Andrew; Rodrigues,Dália dos Prazeres.
Thirty water samples were collected, at two week intervals, from the estuary of the River Cocó. The aim was to characterize the presence, distribution and types of Aeromonas spp, in the estuary of the River Cocó, Ceara, Brazil (03º46'28.83''S e 38º26'36.52''S). Aeromonas were identified in 19 (63%) samples analyzed by plating and CFU counts. Presence/absence tests were positive for 11 (37%) of the samples resulting in the detection of Aeromonas in a total of 23 (77%) of samples. CFU counts varied from < 10 to 1.4 x 10(4) CFU mL-1 . From the isolated strains seven species of Aeromonas were identified: A. caviae (29/69), A. veronii bv. sobria (13/69), A. veronii bv. veronii (8/69), A. trota (6/69), A. media (5/69), A. sobria (4/69) and A. hydrophila and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Estuary; Antibiotics.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200027
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Characterization of mercury-resistant clinical Aeromonas species BJM
Pérez-Valdespino,Abigail; Celestino-Mancera,Martin; Villegas-Rodríguez,Viridiana Lorena; Curiel-Quesada,Everardo.
Mercury-resistant Aeromonas strains isolated from diarrhea were studied. Resistance occurs via mercuric ion reduction but merA and merR genes were only detected in some strains using PCR and dot hybridization. Results indicate a high variability in mer operons in Aeromonas. To our knowledge, this is the first report of mercury-resistant clinical Aeromonas strains.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Mercury resistance; Clinical strains.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400036
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Emerging carbapenem-resistant Aeromonas spp. infections in Cali, Colombia BJID
Rosso,Fernando; Cedano,Jorge Andrés; Parra-Lara,Luis Gabriel; Sanz,Ana María; Toala,Alejandra; Velez,Jolian Fernando; Hormaza,María Paula; Moncada,Pablo Andrés; Correa,Adriana.
ABSTRACT Introduction: Aeromonas species are renowned enteric pathogens with virulence determinants linked to human diseases, such as gastroenteritis, skin, soft-tissue and muscle infections, and septicemia. A recent concern of resistance in this organism has emerged, especially the presence carbapenemases. Herein we describe a case series of emerging carbapenem-resistant Aeromonas species infection in our hospital in Cali, Colombia. Materials and methods: Cases from 2012 to 2018 are reported. Clinical data was abstracted from the clinical charts and laboratory information. Phenotypic detection of resistance was identified using the VITEK®2 system (BioMérieux) and broth microdilution MicroScan WalkAway plus System (Beckman Coulter). CARBA NP-test and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Aeromonas hydrophila; Carbapenems; CphA protein; Real-time polymerase chain.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500336
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Microbiological analysis of critical points in the chicken industry BABT
Cansian,Rogério Luis; Floriani,Salete Teresa Radeski; Valduga,Eunice.
This work is focused on identifying microbial contamination in the scalding asepsis and cooling processes as well as in fresh sausages obtained. Salmonella was identified in two scald water samples but was absent in the water from chiller and in the final product, which might be explained in terms of chlorine addition and temperature reduction. The analysis revealed that MPN of Escherichia coli was in the range of <1-11/mL in the scald water and <1-64/mL in the chiller. Aeromonas sp. count showed 5 to 3.5x10¹ CFU/mL in the scald water and 9 to 3.7x10² CFU/mL in the chiller, probably due to the psycrophile characteristics of Aeromonas and evisceration process. The analysis of fresh sausages chicken revealed a sharp increase in the Aeromonas count...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Bacteria; Chicken; Microbiology; Food quality control.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300011
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Ocorrência de bactérias do gênero Aeromonas em queijo-de-minas frescal artesanal Arq. Bras. Med. Vet. Zootec.
Bulhões,C.C.C.; Rossi Júnior,O.D..
Foram analisadas 160 amostras de queijo-de-minas frescal artesanal, adquiridas no comércio varejista dos municípios de Poços de Caldas - MG e Jaboticabal - SP, a fim de verificar a ocorrência de bactérias do gênero Aeromonas no produto. Oitenta e duas (51,2%) encontravam-se contaminadas pelos microrganismos, com populações que variavam de 5,0×10³ a 4,0×10(5) UFC/grama. Foram identificadas as espécies Aeromonas hydrophila, Aeromonas caviae, Aeromonas schubertii, além de cepas consideradas atípicas. Os resultados evidenciam que bactérias do gênero Aeromonas podem ser veiculadas através do queijo tipo minas frescal artesanal e devem servir de alerta aos serviços de saúde pública.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Queijo-de-minas frescal; Artesanal.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000300017
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Perfil de resistência de Aeromonas spp. isolada no fluxograma de produção do queijo minas frescal industrial e artesanal. Repositório Alice
CERESER, N. D.; ROSSI JÚNIOR, O. D.; MARTINELI, T. M.; SOUZA, V. de; RODRIGUES, L. B.; KERKOFF, J..
Resumo: Bactérias do gênero Aeromonas podem ser isoladas de amostras clínicas, alimentares e ambientais. Para os derivados lácteos a contaminação decorre da presença do agente na matéria prima utilizada para produção, ou devido a condições higiênicas insatisfatórias durante o processamento. Considerando a importância da Aeromonas spp. como patógeno emergente de doenças de origem alimentar, o uso indiscriminado de antimicrobianos nas patologias humanas, além do papel fundamental que o leite e seus derivados representam na alimentação humana, o presente estudo teve por objetivo verificar o comportamento dos isolados frente à ação de antimicrobianos de uso comum. Foram pesquisados produtos e pontos do fluxograma do processamento de derivados lácteos, que...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Queijo Colonial; Queijo Minas Frescal; Resistência antimicrobiana; Antimicrobiano; Queijo; Bactéria; Microrganismo; Resistência a produtos químicos; Antibiótico; Resistência química; Aeromonas; Resistance to antibiotics; Cheese; Drought resistance; Resistance to chemicals.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/973442
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Presence of blaTEM-116 gene in environmental isolates of Aeromonas hydrophila and Aeromonas jandaei from Brazil BJM
Balsalobre,Livia Carminato; Dropa,Milena; de Oliveira,Danielle Escudeiro; Lincopan,Nilton; Mamizuka,Elsa Masae; Matté,Glavur Rogério; Matté,Maria Helena.
It is known that Aeromonas spp. possess different chromosomal β-lactamase genes. Presence and phenotypic expression of blaTEM, blaSHV, and blaCTX-M ESBL-encoding genes were investigated in environmental water isolates of Aeromonas hydrophila and Aeromonas jandaei. Presence of blaSHV and blaCTX-M genes was not observed, and blaTEM gene was verified in 91% of the isolates. Sequencing of 10 fragments showed the occurrence of blaTEM-116.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Environmental isolates.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300023
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The effect of organic osmoprotectors on Aeromonas trota and A. hydrophila grown under high sodium chloride concentrations BJM
Delamare,Ana Paula L.; Dalcin,Thais; Müller,Gabriela; Costa,Sergio Olavo Pinto da; Echeverrigaray,Sergio.
The effect of organic solutes on the growth of Aeromonas trota and A. hydrophila was evaluated. Proline and glutamic acid were not effective as osmoprotectors, but betaine exerts osmoprotection allowing the growth of both strains in inhibitory concentrations of NaCl. Growth kinetics suggests that the halotolerance difference between the strains is associated with the synthesis of osmolytes rather than betaine uptake.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Halotolerance; Betaine; Aeromonas.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500044
Registros recuperados: 23
Primeira ... 12 ... Última
 

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