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Registros recuperados: 9
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Actinobactérias como agentes de controle biológico de fitopatógenos de trigo. Repositório Alice
VENTURA, J. P.; CASTELIANI, A. G. B.; VARGAS HOYOS, H. A.; SANTOS, S. N.; MELO, I. S. de.
Resumo: O trigo (Triticum aestivum L) constitui uma das principais culturas de importância agrícola para o Brasil. Contudo, a ocorrência de doenças como a brusone, tem limitado a produtividade dessa cultura nas regiões produtoras. Desse modo, o objetivo desse trabalho foi avaliar o potencial de uma linhagem Streptomyces sp. CMAA 1647, uma actinobacteria, no controle biológico de fungos fitopatogênicos, principalmente, Pyricularia grisea, agente causal da brusone. A linhagem modelo deste trabalho, Streptomyces sp. CMAA 1647, foi isolada a partir de solos supressivos à brusone e previamente selecionada por inibir o crescimento do agente causal. O potencial de antagonismo da linhagem CMAA 1647 foi avaliado pela técnica de pareamento direto contra os seguintes...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Solos supressivos; Atividade antifúngica; Tratamento de sementes; Antifungal activity; Germination potential; Brusone; Doença de planta; Controle biológico; Trigo; Tratamento de semente; Streptomyces; Suppressive soils; Antifungal agents; Seed treatment; Suppressive soils.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105031
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Antifungal activity of bacterial strains from the rhizosphere of Stachytarpheta crassifolia. Repositório Alice
SANTOS, S. N.; OLIVEIRA, L. K. X.; MELO, I. S. de; VELOSO, E. da S.; ROQUE, M. R. de A..
This study evaluated the antifungal action of biomolecules produced from the secondary metabolism of bacterial strains found in the rhizosphere of semi arid plants against human pathogenic Candida albicans. Crude extracts were obtained using ethyl acetate as an organic solvent and the bioactivity was assessed with a bioautography technique. The results showed that bacterial strains, Alcaligenes faecalis MRbS12 and Bacillus cereus MRbS26, had compounds with antifungal bioactivity. The largest inhibition zones for both compounds were located on spots with Rf values below 0.500, indicating that the molecules possibly had polar characteristics. These results suggested that microorganisms found in the environment could have bioprospecting potential.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biomolecules; Bioautoghaphy; Antifungal activity; Alcaligenes faecalis; Bacillus cereus; Candida albicans; Bactéria; Rizosfera; Controle biológico; Rhizobiales; Antifungal agents; Biological control.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/898255
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Antifungal activity of Pseudomonas frederiksbergensis CMAA 1323 isolated from the Antarctic hair grass Deschampsia antarctica. Repositório Alice
MELO, I. S. de; SOUZA, W. R.; SILVA, L. J.; SANTOS, S. N.; ASSALIN, M. R.; ZUCCHI, T. D.; QUEIROZ, S. C. do N. de.
Abstract: Aims: Epiphytic bacteria, isolated from Deschampsia antarctica, were screened for their potential to inhibit the plant pathogen Botrytis cinerea, the causal agent of gray mold disease of strawberry pseudofruits. This phytopathogenic fungus is more active and the disease is more serious in temperate climate where the temperatures are lower.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Psychrophilic bacteria; Pseudomonas frederiksbergensis; Bioactive compounds; Diketopiperazines; Deschampsia antarctica; Compostos bioativos; Botrytis cinerea; Bactéria; Controle biológico; Diketopiperazines; Deschampsia antarctica; Pseudomonas frederiksbergensis; Psychrophilic bacteria; Antifungal agents.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1054794
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Avaliação da atividade antagonista de bactérias ácido-láticas contra o fungo Botrytis cinerea. Repositório Alice
SMANIOTTO, F.; SANTOS, S. N.; MELO, I. S. de; QUEIROZ, S. C. do N. de.
Resumo: O presente trabalho teve como objetivo avaliar a ação antagonista de bactérias ácido - láticas (BAL), provenientes de formulações simbióticas comerciais e isoladas de alimentos pertencentes à Coleç ão de Microrganismos de Importância Agrícola e Ambiental (CMAA) da Embrapa Meio Ambiente contra o fungo Botrytis cinerea, causador da doença chamada ?podridão cinzenta? nos vegetais. Para isso foram realizadas avaliações antagônicas pela técnica de pareamento direto em placa de Petri. Os isolados crescidos foram repicados em meio BDA e MRS com apenas duas estrias no extremo da placa, foram adicionados discos do fungo B. cinerea no outro extremo e as placas foram incubadas à 28 °C. Nas placas controle foram crescidos somente a linhagem do fungo modelo...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Bactérias ácido?láticas; Fitopatógeno; Probiótico; Acid-lactic bacteria; Phytopathogen; Probiotic; Mofo Cinzento; Botrytis Cinerea; Controle Biológico; Bactéria; Antifungal agents; Gray mold; Biological control; Gray mold.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105232
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Changes in hyphal morphology due to chitosan treatment in some fungal species. Repositório Alice
OLIVEIRA JUNIOR, E. N.; MELO, I. S. de; FRANCO, T. T..
In this work, changes in the hyphal morphology due to chitosan treatment in some fungal species were studied. Scanning electron microscope (SEM) observations revealed that chitosans with molar fraction of acetyl groups (FA 0.16 and 0.18) and degree of polymerization (DP 1,089 and 1,242) had a direct effect on the morphology of the chitosan-treated fungi, reflecting its potential for causing a delay in the growth of Alternaria alternata (500 µg × mL-1), Botrytis cinerea (1,000 µg × mL-1), Penicillium expansum (1,000 µg × mL-1) and Rhizopus stolonifer (500 µg × mL-1). Mycelial aggregation and structural changes such as excessive branching, swelling of the cell wall and hyphae size reduction were observed in the micrographs.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Alternative control of fungi; Antifungal activity; Fungal morphology; Fungo; Doença fúngica; Chitosan; Antifungal agents; Plant pathogenic fungi.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/941619
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Development of mutants of Coniothyrium minitans with improved efficiency for control of Sclerotinia sclerotiorum. Repositório Alice
MELO, I. S. de; MORETINI, A.; CASSIOLATO, A. M. R.; FAULL, J. L..
Coniothurium minitans (CM) is hyperparasitic to Sclerotinia sderotiorum (55), a palhogen of many economically important crops. In this paper, we describe lhe isolation of improved mutants of CM, using a UV - irradiation regime, with altered chitinase production and tolerance to high concentration of iprodione, which are effect:iveagainst Ss. Three out of the 59 mutants obtained inhibited the mycelial growth of CM. Infectivity of sclerotia by the new mutants was assayed by the plant-tissue-based system using carrot segments. More than 80%of sclerotia were colonized by lhe mutants and the wild-type CM. The mutant strains retained ability to produce significant amounts of chitinase. The mutants differed frorn their wild-type strain in appearance, morphology...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Coniothvrium minitans; Iprodione; Resistance; Antibiotic production; Sclerotinia sclerotiorum; Doença fúngica; Controle biológico; Fungo para controle biológico; Biological control; Antifungal agents.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/898276
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In vitro activity of antifungal and antiseptic agents against dermatophyte isolates from patients with tinea pedis BJM
Soares,Maria Magali Stelato Rocha; Cury,Arlete Emily.
The in vitro activity of antifungal and antiseptic agents were evaluated against dermatophytes isolated from patients with tinea pedis. The antifungals studied were: ciclopirox olamine, cetoconazole, tolciclate and terbinafine, and the antiseptics were: povidine iodine (PVPI), propolis, Fungol®, Andriodermol®, and boric acid. The minimum inhibitory concentration (MIC) or the minimal dilution concentration (MDC) was determined by an agar dilution method using modified yeast nitrogen agar base, and the minimum fungicidal concentration (MFC) or minimum fungicidal dilution (MFD) was determined with subcultures on Sabouraud dextrose agar. All drugs studied were active against the dermatophytes at lower concentrations than those used in products and/or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dermatophytes; Antifungal agents; Antiseptic agents; Susceptibility testing; Tinea pedis.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200012
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Mining of the endophytic microbiome for novel biosynthetic genes and antifungal metabolites. Repositório Alice
CARRION, V. J.; PÉREZ-JARAMILLO, J.; CORDOVEZ, V.; HOLLANDER, M. de; TRACANNA, V.; MENDES, L. W.; MENDES, R.; MEDEMA, M. H.; RAAIJMAKERS, J. M..
Endophytic microbes have been proposed to contribute to a range of plant support functions, including nutrient acquisition and disease protection. To date, the molecular mechanisms underlying disease protection in the endosphere have only been studied for individual culturable organisms. Here, we conducted a systematic microbiome-wide perspective on the taxonomic diversity and functional potential of the endophytic microbiome of plants grown in an agricultural soil that is naturally suppressive to damping-off disease caused by the fungal root pathogen Rhizoctonia solani. Metagenomic analyses showed an enrichment of Burkholderiaceae, Chitinophagaceae and Xanthomonadaceae in the endosphere of plants grown in disease-suppressive soil challenged with the...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Microbiome; Antifungal agents; Metagenomics.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103500
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The importance of chorismate mutase in the biocontrol potential of Trichoderma parareesei. Repositório Alice
PÉREZ, E.; RUBIO, M. B.; CARDOZA, R. E.; GUTIÉRREZ, S.; BETTIOL, W.; MONTE, E.; HERMOSA, R..
Species of Trichoderma exert direct biocontrol activity against soil-borne plant pathogens due to their ability to compete for nutrients and to inhibit or kill their targets through the production of antibiotics and/or hydrolytic enzymes. In addition to these abilities, Trichoderma spp. have beneficial effects for plants, including the stimulation of defenses and the promotion of growth. Here we study the role in biocontrol of the T. parareesei Tparo7 gene, encoding a chorismate mutase (CM), a shikimate pathway branch point leading to the production of aromatic amino acids, which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites. We isolated T. parareesei transformants with the Tparo7...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Tparo7 gene; 2-phenylethanol; Tyrosol; Trichoderma; Controle biológico; Shikimate pathway; Gene silencing; Antifungal agents; Phenylethyl alcohol; Salicylic acid.
Ano: 2015 URL: http://dx.doi.org/10.3389/fmicb.2015.01181
Registros recuperados: 9
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