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Registros recuperados: 39
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Action mechanisms involved in the bioprotective effect of Lactobacillus harbinensis K.V9.3.1.Np against Yarrowia lipolytica in fermented milk ArchiMer
Mieszkin, Sophie; Hymery, Nolwenn; Debaets, Stella; Coton, Emmanuel; Le Blay, Gwenaelle; Valence, Florence; Mounier, Jerome.
The use of lactic acid bacteria (LAB) as bioprotective cultures can be an alternative to chemical preservatives or antibiotic to prevent fungal spoilage in dairy products. Among antifungal LAB, Lactobacillus harbinensis K.V9.3.1Np showed a remarkable antifungal activity for the bioprotection of fermented milk without modifying their organoleptic properties (Delavenne et al., 2015). The aim of the present study was to elucidate the action mechanism of this bioprotective strain against the spoilage yeast Yarrowia lipolytica. To do so, yeast viability, membrane potential, intracellular pH (pHi) and reactive oxygen species (ROS) production were assessed using flow cytometry analyses after 3, 6 and 10 days incubation in cell-free supernatants. The tested...
Tipo: Text Palavras-chave: Lactobacillus harbinensis; Yarrowia lipolytica; Bioprotection; Antifungal; Action mechanisms; Dairy products.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00373/48390/48563.pdf
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Acute disseminated paracoccidioidomycosis in a 3 year-old child BJID
Grossklaus,Daciene de Arruda; Tadano,Tomoko; Breder,Sandra Assis; Hahn,Rosane Christine.
Clinical cases involving paracoccidioidomycosis in children, diagnosed in Mato Grosso State, in the central western region of Brazil, are rare despite the state being classified with a moderate to high incidence. We describe a clinical case of infant acute disseminated Paracoccidioidomycosis in Mato Grosso State, Brazil, highlighting the importance of early differential diagnosis from other severe pathologies, thus contributing to the survival of compromised patients. A 3 year-old male child, weighing 12.8 Kg, originating from Vila Rica, MT, Brazil. The patient presented intermittent 40ºC fever evolving over 40 days, dry cough and painless bilateral cervical adenomegaly, showing no signs of inflammation. This was associated with diarrhea, distension and...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Acute paracoccidioidomycosis; Children; Antifungal.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300018
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Antifungal activity against Cryptococcus neoformans strains and genotoxicity assessment in human leukocyte cells of Euphorbia tirucalli L. BJM
Oliveira,Luís Flávio Souza de; Fuentefria,Alexandre Meneghello; Klein,Fernanda da Silva; Machado,Michel Mansur.
In the last times, focus on plant research has increased all over the world. Euphorbia tirucalli L., a plant known popularly as Aveloz, and originally used in Africa, has been drawing attention for its use in the United States and Latin America, both for use as an ornamental plant and as a medicinal plant. E. tirucalli L. is a member of the family Euphorbiaceae and contains many diterpenoids and triterpenoids, in particular phorbol esters, apparently the main constituent of this plant, which are assumed to be responsible for their activities in vivo and in vitro. The in vitro antifungal activities of Euphorbia tirucalli (L.) against opportunistic yeasts were studied using microbroth dilution assay. The results showed that aqueous extract and latex...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Euphorbia tirucalli L.; Aveloz; Antifungal; Cryptococcus neoformans.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400027
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Antifungal activity of acidic electrolyzed water against strawberry postharvest molds (Fragaria x ananassa Duch cv. Camarosa) Acta Agron. (Palmira)
Guerra-Sierra,Beatriz Elena; Sandoval-Meza,Adriana Ximena; García-Sanchéz,Liliana Torcoroma.
Abstract The aim of this word was to assess the in vitro antifungal activity of acidic electrolyzed water (AEW) and the required contact time to inhibit the development of post-harvest molds common in strawberry fruit. Ninety- four strawberry samples were used. A five-fold assay in a randomized design was carried out using standard inoculum of fungal conidia, which were subjected to an array of contact times and AEW concentrations, using a controlled agitation (120 r.p.m) at 23°C. The conidia treated with AEW were grown on potato dextrose agar and their germination was evaluated. Mycelial growth of the treated samples was measured and compared against conidia control samples treated with distilled water instead of AEW. Botrytis cinerea and Rhizopus...
Tipo: Journal article Palavras-chave: Acidic electrolyzed water; Antifungal; Botrytis cinerea; Post-harvest molds; Rhizopus stolonifer.
Ano: 2019 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122019000200126
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Antifungal activity of Cymbopogon winterianus jowitt ex bor against Candida albicans BJM
Oliveira,Wylly Araújo de; Pereira,Fillipe de Oliveira; Luna,Giliara Carol Diniz Gomes de; Lima,Igara Oliveira; Wanderley,Paulo Alves; Lima,Rita Baltazar de; Lima,Edeltrudes de Oliveira.
Candida albicans is an opportunistic yeast and a member of the normal human flora that commonly causes infections in patients with any type of deficiency of the immune system. The essential oils have been tested for antimycotic activity and pose much potential as antifungal agents. This work investigated the activity of the essential oil of Cymbopogon winterianus against C. albicans by MIC, MFC and time-kill methods. The essential oil (EO) was obtained by hydrodistillation using a Clevenger-type apparatus. It was tested fifteen strains of C. albicans. The MIC was determined by the microdilution method and the MFC was determined when an aliquot of the broth microdilution was cultivated in SDA medium. The phytochemical analysis of EO showed presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Antifungal; Cymbopogon winterianus.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200004
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ANTIFUNGAL ACTIVITY SCREENING OF ANTARCTIC ACTINOBACTERIA AGAINST PHYTOPATHOGENIC FUNGI Acta biol.Colomb.
SANTOS,Andrés; NÚÑEZ-MONTERO,Kattia; LAMILLA,Claudio; PAVEZ,Mónica; QUEZADA-SOLÍS,Damián; BARRIENTOS,Leticia.
ABSTRACT The extreme weather conditions in the Antarctic have exerted selective pressures favoring differential features in bacteria to survive this untapped environment (i.e., antibiotic molecules). Notably, higher chances of antibiotic discovery from extremophiles have been proposed recently. Although new organic and environmentally friendly sources for helping in the control of plant pathogenic fungi are necessary, the information about anti-phytopathogenic applications of extremophile microorganisms from untapped environments is limited. In this study, we determined the antifungal effect of actinobacterial strains isolated from Antarctic soils and sediments. Co-culture inhibition assays and Minimum Inhibitory Concentration (MIC) determination revealed...
Tipo: Journal article Palavras-chave: Antagonism; Antarctic bacteria; Antifungal; Extremophile; Plant pathogen.
Ano: 2020 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000200353
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Antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils Anais da ABC (AABC)
PINTO,MARIA E.A.; ARAÚJO,STHÉFANE G.; MORAIS,MARCELA I.; SÁ,NÍVEA P.; LIMA,CAROLINE M.; ROSA,CARLOS A.; SIQUEIRA,EZEQUIAS P.; JOHANN,SUSANA; LIMA,LUCIANA A.R.S..
ABSTRACT Fatty acid methyl esters (FAMEs) were obtained from vegetable oils of soybean, corn and sunflower. The current study was focused on evaluating the antifungal activity of FAMEs mainly against Paracoccidioides spp., as well as testing the interaction of these compounds with commercial antifungal drugs and also their antioxidant potential. FAMEs presented small IC50 values (1.86-9.42 μg/mL). All three FAMEs tested showed antifungal activity against isolates of Paracoccidioides spp. with MIC values ranging from 15.6-500 µg/mL. Sunflower FAMEs exhibited antifungal activity that extended also to other genera, with an MIC of 15.6 μg/mL against Candida glabrata and C. krusei and 31.2 μg/mL against C. parapsilosis. FAMEs exhibited a synergetic effect with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Antioxidant; Fatty acid methyl esters; Vegetable oils.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401671
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Antifungal defensins and their role in plant defense. Repositório Alice
LACERDA, A. F.; VASCONCELOS, E. A. R.; PELEGRINI, P. B.; SA, M. F. G. de.
bitstream/item/108397/1/fmicb-05-00116.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Plant defensins; Antifungal; Phytopathogens; Peptide structure; Peptide function; Transgeny.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/994966
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Antifungal effect of lavender honey against Candida albicans, Candida krusei and Cryptococcus neoformans IPB - Escola Superior Agrária
Estevinho, Leticia M.; Afonso, Sílvia Marlene Esteves; Feás, Xesús.
Monofloral lavender honey samples (n=30), were analyzed to test antifungal effect against Candida albicans, Candida krusei, and Cryptococcus neoformans. The specific growth rates (μ) showed that all the yeast growths were reduced in the presence of honey. The honey concentration (% w/v) that inhibited 10% of the yeasts growth (Xmin) ranged from 31.0% (C. albicans), 16.8% (C. krusei) and 23.0% (C. neoformans). A synthetic honey solution was also tested to determine antifungal activity attributable to sugars. The presence of synthetic honey in the C. krusei culture medium at concentrations above 58.0% (w/v) was established as Xmin, while C. albicans and C. neoformans were more resistant, since Xmin values were not reached over the ranged tested (10–60%,...
Tipo: Article Palavras-chave: Antifungal; Lavender.
Ano: 2011 URL: http://hdl.handle.net/10198/5840
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Antifungal susceptibility of yeasts isolated from anthropogenic watershed Anais da ABC (AABC)
MILANEZI,ANA C.M.; WITUSK,JOÃO P.D.; VAN DER SAND,SUELI T..
Abstract Yeasts are unicellular fungi widespread in the environment, and studies suggest that there is a positive correlation between yeast and polluted aquatic environments. The aim of this study was to analyze the diversity and resistance of yeasts isolated from water samples collected along the Arroio Dilúvio in Porto Alegre to antifungals. Yeast isolates from the Arroio Dilúvio were subjected to susceptibility assays against antifungals using the minimum inhibitory concentration (MIC) test, and amplification of the ITS1-5.8S-IT2 region; in addition, the ITS-5.8S region was sequenced for some of the isolates. The amplification product was subjected to PCR-RFLP, and the restriction profile allowed the construction of a dendrogram. Susceptibility tests...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Antifungal; Watershed; Resistance.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100601
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Antimicrobial activity of bergenin from Endopleura uchi (Huber) Cuatrec Acta Amazonica
Silva,Saulo L. da; Oliveira,Viviane Guedes de; Yano,Tomomasa; Nunomura,Rita de Cássia Saraiva.
Endopleura uchi (Huber) Cuatrec. is an Amazon species traditionally used as treatment for inflammations and female disorders. Bergenin was isolated from ethyl acetate fraction of bark of E. uchi by using column chromatography over sephadex LH-20 and then silica gel 60 flash. Its structure was identified on the basis of its NMR spectra. The antimicrobial activity of bergenin and fractions of methanol extract of E. uchi were evaluated against ATCC microorganisms (Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Candida albicans, C. guilliermondii, Aspergillus flavus, A. nidulans). Clinically isolated strains of all of these microorganisms, along with C. tropicalis, A. niger, Shigella sonnei,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial; Antifungal; Candida; Humiriaceae; Uxi-amarelo.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672009000100019
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Antimicrobial activity of honeys from two stingless honeybee species and Apis mellifera (Hymenoptera: Apidae) against pathogenic microorganisms Acta Amazonica
Cruz,Carolinie Batista Nobre da; Pieri,Fabio Alessandro; Carvalho-Zilse,Gislene Almeida; Orlandi,Patrícia Puccinelli; Nunes-Silva,Carlos Gustavo; Leomil,Luciana.
Honeys are described possessing different properties including antimicrobial. Many studies have presented this activity of honeys produced by Apis mellifera bees, however studies including activities of stingless bees honeys are scarce. The aim of this study was to compare the antimicrobial activity of honeys collected in the Amazonas State from Melipona compressipes, Melipona seminigra and Apis mellifera against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Chromobacterium violaceum, and Candida albicans. Minimum inhibitory concentrations were determined using the agar dilution method with Müller-Hinton agar (for bacteria) or Saboraud agar (for yeast). Staphylococcus aureus and E. faecalis were inhibited by all honeys at concentrations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melipona; Antibacterial; Antifungal; Natural products; Functional food.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672014000200015
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Antimicrobial activity of some medicinal plants from the cerrado of the central-western region of Brazil BJM
Violante,Ivana Maria Póvoa; Hamerski,Lidilhone; Garcez,Walmir Silva; Batista,Ana Lucia; Chang,Marilene Rodrigues; Pott,Vali Joana; Garcez,Fernanda Rodrigues.
Ethanol extracts from six selected species from the Cerrado of the Central-Western region of Brazil, which are used in traditional medicine for the treatment of infectious diseases and other medical conditions, namely Erythroxylum suberosum St. Hil. (Erythroxylaceae), Hyptis crenata Pohl. ex Benth. (Lamiaceae), Roupala brasiliensis Klotz. (Proteaceae), Simarouba versicolor St. Hil. (Simaroubaceae), Guazuma ulmifolia Lam. (Sterculiaceae) and Protium heptaphyllum (Aubl.) March. (Burseraceae), as well as fractions resulting from partition of these crude extracts, were screened in vitro for their antifungal and antibacterial properties. The antimicrobial activities were assessed by the broth microdilution assay against six control fungal strains, Candida...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Antibacterial; Antimicrobial; Artemia salina; Cerrado.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400009
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Antimicrobial Compounds from Eukaryotic Microalgae against Human Pathogens and Diseases in Aquaculture ArchiMer
Falaise, Charlotte; Francois, Cyrille; Travers, Marie-agnes; Morga, Benjamin; Haure, Joel; Tremblay, Rejean; Turcotte, Francois; Pasetto, Pamela; Gastineau, Romain; Hardivillier, Yann; Leignel, Vincent; Mouget, Jean-luc.
The search for novel compounds of marine origin has increased in the last decades for their application in various areas such as pharmaceutical, human or animal nutrition, cosmetics or bioenergy. In this context of blue technology development, microalgae are of particular interest due to their immense biodiversity and their relatively simple growth needs. In this review, we discuss about the promising use of microalgae and microalgal compounds as sources of natural antibiotics against human pathogens but also about their potential to limit microbial infections in aquaculture. An alternative to conventional antibiotics is needed as the microbial resistance to these drugs is increasing in humans and animals. Furthermore, using natural antibiotics for...
Tipo: Text Palavras-chave: Biological activity; Antimicrobial; Antibacterial; Antifungal; Antiviral; Biological activity; Haslea; Microalgae; Sustainable aquaculture.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00349/46052/45719.pdf
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Applications of Plackett–Burman and Central Composite Design for the Optimization of Novel Brevundimonas diminuta KT277492 Chitinase Production, Investigation of its Antifungal Activity BABT
Warda,E. Ashour; Abeer,A. Abd El Aty; Eman,R. Hamed; Mahmoud,A. Swelim; Ahmed,I. El-Diwany.
ABSTRACT Biological control strategy which can damage chitin, a vital component of pathogenic fungi and arthropods promises a safe solution for many fungal problems. And it’s more favorable than chemicals which increase health risks and environmental problems. Thus, the chitinase producers appear potential candidates of biological control of pathogenic fungi. Brevundimonus diminuta KT277492 is a new isolate that has been isolated recently from Egyptian soil. Significant factors that affecting the chitinase enzyme production were studied and optimized using Plackett-Burman and Response Surface Methodology (RSM). As a result, maximum production of chitinase enzyme was 832.87 IUL-1, this result presented about 8.767-fold increase in the enzyme production. In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Brevundimonas diminuta KT277492; Optimization; Plackett–Burman; Central composite design; Antifungal.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100429
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BIOEFFICACY OF A COMMON WEED Datura metel AGAINST Colletotrichum gloeosporioides Planta Daninha
KARIM,M.; JABEEN,K.; IQBAL,S.; JAVAID,A..
ABSTRACT Mango (Mangifera indica) is one of the most important trees grown mainly in the tropical and subtropical countries. Anthracnose disease caused by Colletotrichum gloeosporioides, is the most damaging disease causing reduction of flower set and yield losses in mango. The present study was designed to evaluate antifungal activity of Datura metel extracts against C. gloeosporioides. Extracts (1% to 3%) of different parts of D. metel viz. leaves, seeds and roots were assessed for their antifungal potential. Methanolic seed extract of D. metel depicted the highest antifungal potential against the test fungus. All the applied concentrations effectively reduced growth of the test fungus. However, 1.5% conc. of methanolic seeds extract showed maximum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; GC-MS; Methanolic extracts; Phytochemical analysis.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100244
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Characterization of a chitinase with antifungal activity from a native Serratia marcescens B4A BJM
Zarei,Mandana; Aminzadeh,Saeed; Zolgharnein,Hossein; Safahieh,Alireza; Daliri,Morteza; Noghabi,Kambiz Akbari; Ghoroghi,Ahmad; Motallebi,Abbasali.
Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indicated an optimum activity in pH 5 at 45ºC. Enzyme was stable in 55ºC for 20 min and at a pH range of 3-9 for 90 min at 25ºC. When the temperature was raised to 60ºC, it might affect the structure of enzymes lead to reduction of chitinase activity. Moreover, the Km and Vmax values for chitin were 8.3 mg/ml and 2.4 mmol/min, respectively. Additionally, the effect of some cations and chemical compounds were found to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Characterization; Chitinase; Serratia marcescens B4A.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300022
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Characterization of bacterial endophytes from Myanmar medicinal plants for antimicrobial activity against human and plant pathogens BJPS
Myo,Ei Mon; Maung,Chaw Ei Htwe; Mya,Khin Mar; Khai,Aye Aye.
This research aimed to investigate the antagonistic activity of the bacterial endophytes from Myanmar medicinal plants. Thirty-one bacterial isolates were isolated from Myanmar medicinal plants: Tinospora cordifolia (Wild.) Miers., Catharanthus roseus G. Don., Tectona hamiltoniana Wall. and Boscia variabilis Collett & Hemsl. (Capparaceae). Dual culture and agar well diffusion methods were used for antimicrobial assay. One endophyte from Catharanthus roseus and two bacterial isolates from Boscia variabilis Collett & Hemsl. (Capparaceae) had not only the antibacterial activity towards the human pathogenic bacteria but also the antifungal activity against the plant pathogenic fungi. From 16S rRNA sequencing, one strain from Catharanthus roseus G. Don....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial; Antifungal; Culture conditions; Endophytes; Medicinal plants.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100513
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Chemical, biochemical, and microbiological aspects of chitosan quaternary salt as active coating on sliced apples Ciênc. Tecnol. Aliment.
Britto,Douglas de; Assis,Odilio Benedito Garrido.
The biocompatibility of chitosan and chitosan quaternary salt coatings was evaluated for use as edible coatings for sliced apple. Measurement of water loss, color change, and fungal growth appearance were monitored as a function of time. A significant brownish effect was observed on chitosan coated slices, varying greatly from L* = 76.5 and Hue angle = 95.9° (t = 0) to L* = 45.3 and Hue angle = 69.8° (t = 3 days), whilst for TMC coated samples the variation was considerable lower (L* = 74.1; Hue angle = 95.0°) to (L* = 67.0; Hue angle = 83.8°) within the same period. The hydrosoluble derivative N,N,N-trimethylchitosan demonstrated good antifungal activity against P. expansum although highly dependent on the polymer properties such as degree of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trimethyl chitosan; Quaternary salts; Antifungal; Minimally processed food; Edible coating.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000300026
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Chemical characterization of Lippia alba essential oil: an alternative to control green molds BJM
Glamočlija,Jasmina; Soković,Marina; Tešević,Vele; Linde,Giani Andrea; Colauto,Nelson Barros.
The essential oil of Lippia alba is reported as an antifungal against human pathogenic microorganisms but few articles report its use as an alternative to synthetic fungicides on green mould control. The objective of this study was to determine chemical characteristics of L. alba essential oil and its antifungal activity against green molds as an alternative to synthetic fungicides. Essential oil was extracted by Clevenger hydrodistillation, characterized by GC-MS analysis, and the structure of the main compounds confirmed by ¹H and 13C-NMR spectroscopy. Microdilution assays evaluated the essential oil minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Commercial fungicides Ketoconazole and Bifonazole were used as control....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Essential oil; Neral; Geranial; Lippia alba.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400041
Registros recuperados: 39
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