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A single strain of Mycobacterium bovis bacillus Calmette-Guérin (BCG) grown in two different media evokes distinct humoral immune responses in mice BJMBR
Petricevich,V.L.; Ueda,C.; Alves,R.C.B.; da Silva,M.A.; Moreno,C.; Melo,A.R.; Dias da Silva,W..
Two attenuated bacillus Calmette-Guérin (BCG) preparations derived from the same Moreau strain, Copenhagen but grown in Sauton medium containing starch and bacto-peptone (onco BCG, O-BCG), or asparagine (intradermal BCG, ID-BCG), exhibited indistinguishable DNA sequences and bacterial morphology. The number of viable bacilli recovered from spleen, liver and lungs was approximately the same in mice inoculated with the vaccines and was similarly reduced (over 90%) in mice previously immunized with either BCG vaccine. The humoral immune response evoked by the vaccines was, however, distinct. Spleen cell proliferation accompanying the growth of bacilli in tissue was significantly higher in mice inoculated with O-BCG. These cells proliferated in vitro upon...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus Calmette-Guérin; BCG; Tuberculosis; Mycobacterium bovis; Vaccine.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100010
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Bacillus Calmette-Guérin immunization in infants born to HIV-1-seropositive mothers IRD
Lallemant-Le-Coeur, S.; Lallemant, Marc; Cheynier, D.; Nzingoula, S.; Drucker, J.; Larouze, B..
Tipo: Text Palavras-chave: SANTE PUBLIQUE; ENQUETE; NOUVEAU NE; SIDA; VACCINATION; BCG; TUBERCULOSE; EFFET SECONDAIRE; HIV-1.
Ano: 1991 URL: http://www.documentation.ird.fr/hor/fdi:31746
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Histopathological, immunohistochemical and ultraestructural evaluation of inflammatory response in Arius genus fish under experimental inoculation of BCG BABT
Sado,Ricardo Yuji; Matushima,Eliana Reiko.
The aim of this study was to evaluate the inflammatory response kinetics after experimental inoculation with BCG in the primitive Arius sp. fish. The BCG was applied through the intramuscular injection in the caudal peduncular region, and the samples were collected for the analyses at days 1, 3, 7, 14, 21, and 33 post-injection. Acute phase inflammatory infiltrate was characterized by the predominant mononuclear cells, intersticial edema, and muscular tissue necrosis. As the inflammatory response evolved, a large number of multinuclear giant cells were formed containing the BCG. These giant cells were positive for the S100 protein at the histochemical analysis, which demonstrate the macrofage activity, confirmed by the ultra-structural analysis showing the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Arius; Phylogeny; BCG; Inflammation; Giant cells.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500009
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Influence of initial L-asparagine and glycerol concentrations on the batch growth kinetics of Mycobacterium bovis BCG BJM
Leal,Maria Betania Batista; Baruque-Ramos,Júlia; Hiss,Haroldo; Paz,Marcelo Fossa da; Sakai,Maria Cristina; Vassoler,Umbelina Macedo; Arauz,Luciana Juncioni de; Raw,Isaías.
The influences of the L-asparagine and glycerol initial concentrations in Sauton medium on the productivities of biomass and colony forming units were studied. The submerged batch cultivations of Mycobacterium bovis were carried out in a 20 L bioreactor. The L-asparagine and glycerol initial concentrations of 4.54 g/L and 25 mL/L, respectively, corresponded to the best biomass productivity, namely 2.5 g/L.day. On the other hand, the concentrations of 2.27 g/L and 25 mL/L, respectively, led to the highest productivity in terms of colony forming units, namely 2.7·10(6) colonies/mg.day. In addition, by means of the relative consumption analysis of L-asparagine and glycerol (50 and 26% respectively), it was concluded that the concentrations of such components...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BCG; Mycobacterium bovis; Culture medium; Vaccine; Submerged batch cultivation.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300013
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Preservation of cytotoxic granule production in response to mycobacterial antigens by T-lymphocytes from vertically HIV-infected Brazilian youth on effective combined antiretroviral therapy BJID
Arrym,Mauro Pedromonico; Alves,Paulo César Martins; Castelhano,Mariana Virginello; Mazzola,Taís Nitsch; Lemos,Renata Muller Banzato Pinto de; Zaccariotto,Tânia Regina; Levy,Carlos Emilio; Guimarães,Fernando; Silva,Marcos Tadeu Nolasco da.
ABSTRACT Background: HIV infection harms adaptive cellular immunity mechanisms. Long-term virological control by combined antiretroviral therapy (cART) reduces the risk of mycobacterial infections. Thus, we aimed to study cellular responses to mycobacterial antigens in 20 HIV-infected adolescents with at least one year of virological control (HIV-RNA <40 copies/mL) and 20 healthy adolescents. Methods: We evaluated CD8 and γδ T-cell degranulation by measurement of CD107a membrane expression after stimulation with lysates from BCG (10 µg/mL) and H37RA Mycobacterium tuberculosis (Mtb, 10 µg/mL). Immune activation and antigen-presenting ability were also assessed by determination of HLA-DR, CD80, and CD86 markers. Results: TCR γδ T-cell CD107a expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV/Aids; BCG; Youth; Tuberculosis; Citotoxicity; Immunologic.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300151
Registros recuperados: 5
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