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Registros recuperados: 18
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A PRELIMINARY STUDY FOR ELIMINATION OF CONTAMINATED BABESIA MICROTI FROM THE MODEL OF HUMAN PLASMA FRACTION OAK
Nishisaka, M.; Igarashi, Ikuo; Fujisaki, Kozo; Miyahara, T.; Ogata, Y.; Nagasawa, Hideyuki; Mikami, Takeshi.
Palavras-chave: Babesia microti; Human plasma fraction; Filtration.
Ano: 2002 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/629
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Anil-Babesia rodhaini Monoclonal Antibodies: Effect against Babesia rodhaini and Cross-reactivity with Human Starin of Babesia microti OAK
Claveria, Florencia G.; Omata, Yoshitaka; Takehara, Tokuma; Saito, Atsushi; Maki, Yoshiyuki; Igarashi, Ikuo; Suzuki, Naoyoshi.
Anti-Babesia rodhaini monoclonal antibodies (mAb), namely: 1-E7, 2-H2 and 3-B8, significantly suppressed the development of high parasitemia in BALB/c mice infected with B. rodhaini and all mAb-treated mice survived the infection. While, only monoclonal antibody 3-B8 showed some inhibitory effect against Babesia microti, with mice showing high parasitemia of 18.04 ± 2.69 %, at 9 days post-exposure. Westernblot analysis of B. rodhaini and B. microti parasite extract reacted with anti-B. rodhaini monoclonal antibodies showed cross-reactive bands of molecular weights 62 and 55 kilodaltons. Comparison of the antigenic components of B. rodhaini and B. microti using polyspecific sera revealed several shared or common parasite antigens of molecular weights 62,...
Palavras-chave: Monoclonal antibodies; Cross-reactivity; Babesia rodhaini; Babesia microti.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/171
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Artesunate, a potential drug for treatment of Babesia infection OAK
Goo, Youn-Kyoung; Terkawi, M. Alaa; Jia, Honglin; Aboge, G. Oluga; Ooka, Hideo; Kim, Suk; Igarashi, Ikuo; Nishikawa, Yoshifumi; Xuan, Xuenan.
The effect of artesunate, a water-soluble artemisinin derivative, against Babesia species, such as Babesia bovis, Babesia gibsoni, and Babesia microti was studied. Cultures of B. bovis and B. gibsoni were treated with 0.26 μM, 2.6 μM, 26 μM, and 260 μM artesunate, and the growth-inhibitory effect was shown in over 2.6 μM artesunate in day 4 and day 3 post-subculture for B. bovis and B. gibsoni, respectively, in dose-dependent manner. In vivo experiment for B. microti, strong inhibition effects were observed in mouse groups treated with over 1.0 mg/kg body weight of artesunate on day 9 and 10 post-infection. These results suggest that artesunate could be a potential drug for Babesia infection.
Palavras-chave: Artesunate; Babesia bovis; Babesia gibsoni; Babesia microti.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2820
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Changes of splenic interleukin-12p70 concentration in mice infected with Babesia microti and Babesia rodhaini OAK
Ohmori, Takashi; Tamahara, Satoshi; Thi, Pham Ngoc; Tomihari, Mizuki; Shimada, Terumasa; Matsuki, Naoaki; Ono, Kenichiro.
Interleukin-12 (IL-12) is one of the most important factors for the host’s protective immune response against protozoan infection. To evaluate the effect of bioactive form of IL-12(IL-12p70), expression of IFN-AmRNA and IL-4 mRNA in splenic CD4 positive T cells, anti-parasite delayed type hypersensitivity (DTH), and serum and splenic IL-12p70 concentration were examined in Babesia microti and B. rodhaini infected mice. The expression of IFN-AmRNA as a marker of Th1 cell differentiation was detected in splenic CD4 positive T cells in B. microti infected mice. Activation of DTH response as a marker of cell-mediated immune response was also observed from 2 days after the infection with B. microti, while no response was detected in B. rodhaini infected mice....
Palavras-chave: Babesia microti; Babesia rodhaini; Cell-mediated immunity; Early phase of infection; IL-12p70; Th1 cell differentiation.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/150
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Comparison of Damage to Kidneys and Liver Caused by Lethal Babesia rodhaini Infection and Non-lethal Babesia microti Infection in Mice OAK
Igarashi, Ikuo; Hosomi, Takushi; Kaidoh, Toshiyuki; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Aikawa, Masamichi.
Palavras-chave: Babesia rodhaini; Babesia microti; Kidneys; Liver; Mice infection.
Ano: 1993 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/198
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Different Activation of Lymphocytes in Reaction to Babesia microti Infection in iNOS-/- and Wildtype Mice OAK
Remer, Katharina; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行.
Palavras-chave: Lymphocytes; Babesia microti; Spleen; INOS-/- mice; CD4+ cells; CD8+ cells; IFN-γ; Antibody-titer.
Ano: 1997 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/263
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Effect of Oligomannose-Coated Liposome-Based Vaccine on Rodent Babesiosis OAK
Ishigame, T; Takabatake, N; Iseki, H; Ota, N; Koyama, A; Igarashi, I; Nishikawa, Y; Ikehara, Y; Kojima, N; Yokoyama, N; 五十嵐, 郁男; 西川, 義文; 横山, 直明.
Oligomannose-coated liposome (OML)-based vaccines have been reported to induce Th1-based immunity against entrapped antigens in immunized animals and to show protective effects against several protozoal diseases. In the present study, we produced a recombinant Babesia rodhaini ribosomal phosphoprotein P0 (rBrP0) that showed immunological cross reactivity with another rodent Babesia parasite, B. microti. We evaluated the efficacy of vaccination with OML-entrapped rBrP0 on B. rodhaini and B. microti infections in mice. Prior immunization with the OML-based rBrP0 vaccine, or with a Freund’s adjuvant-based rBrP0 vaccine, failed to demonstrate any protective effect against lethal infection with B. rodhaini, but the OML-based vaccine did induce protective...
Palavras-chave: Oligomannose-coated liposomes (OML); Vaccine; Babesiosis; Babesia rodhaini; Babesia microti; P0.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2383
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Experimental infection of dogs with Babesia microti OAK
Ohmori, T.; Uetsuka, K.; Nunoya, T.
Palavras-chave: Babesia microti; Dog; Experimental infection; Polymerase chain reaction; Indirect immunofluorescence test.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3132
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Expressions of Gamma-interferon and Interleukin-4 Messenger RNA in Splenic L3T4+ T Cells from Babesia microti- and B. rodhaini- Infected Mice OAK
Shimada, Terumasa; Shikano, Sojin; Hashiguchi, Rie; Ohashi, Fumihito; Matsuki, Naoaki; Ono, Kenichiro.
Palavras-chave: Babesia microti; Babesia rodhaini; Splenic helper T cell subset.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/242
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Identification, Cloning and Characterization of BmP41, a Common Antigenic Protein of Babesia microti OAK
MASATANI, Tatsunori; OOKA, Hideo; TERKAWI, Mohamad A.; CAO, Shinuo; LUO, Yuzi; ASADA, Masahito; HAYASHI, Kei; NISHIKAWA, Yoshifumi; XUAN, Xuenan; 西川, 義文; 玄, 学南.
Babesia microti is a rodent tick-borne blood parasite and the major causative agent of emerging human babesiosis. Here, we identified a candidate of common antigenic protein BmP41 of B. microti by serological screening of cDNA library of human-pathogenic Gray strain with antisera against rodent Munich strain. Immunofluorescent antibody test using mouse anti-recombinant BmP41 (rBmP41) serum revealed that native BmP41 was expressed in each of the developmental stages of B. microti merozoites. An enzyme-linked immunosorbent assay (ELISA) using rBmP41 detected specific antibodies in sera from hamsters infected with B. microti Gray strain and mice infected with B. microti Munich strain. Taken together, BmP41 could be a promising universal serological marker for...
Palavras-chave: Babesia microti; BmP41; Common antigen; ELISA; Immunoscreening.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3917
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NITRIC OXIDE IS INVOLVED IN EARLY HOST DEFENSE AGAINST A PRIMARY INFECTION WITH BABESIA MICROTI IN MICE OAK
Remer, Katharina; Igarashi, Ikuo; Toyoda, Yutaka; Suzuki, Naoyoshi.
Palavras-chave: Babesia microti; Nitric oxide; INOS-/- mice; Aminoguanidine; Parasitemia; Hematocrit; Interferon-γ.
Ano: 2003 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/633
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PCR detection of Babesia microti and Babesia divergens in ticks OAK
Skotarczak, Bogumila; ACichocka, Gnieszka.
We investigated the presence of Babesia microti and B. divergens in Ixodes ricinus ticks collected in a Lyme borreliosis endemic area of Northern Poland. We used as the PCR target the fragment from a gene encoding the nuclear small-subunit ribosomal RNA (SS-rDNA). We have made a thorough analysis of the quantitative and rate per cent occurrence Ixodes ricinus from two areas of forest in province of Szczecin, known as highly recreative and frequented of many people resort. A total of 533 specimens collected in 13.3% was detected DNA of B. microti and in 3.0% of B. divergens.
Palavras-chave: Babesia microti; Babesia divergens; Ixodes ricinus; PCR.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/135
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Role of CD4⁺ T Cells in the Control of Primary Infection with Babesia microti in Mice OAK
Igarashi, Ikuo; Waki, Seiji; Ito, Mamoru; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; 五十嵐, 郁男.
Palavras-chave: Babesia microti; Nude mice; CD4⁺ T Cells; Primary infection; Gamma interferon.
Ano: 1994 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/218
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Studies of Liver of Mice in the Course of an Experimental Infection with Babesia microti. Ⅱ : Histopathological Studies on Oxidative and Antioxidative Enzymes OAK
Kuzna-Grygiel, Wanda; Kolodziejczyk, Lidia.
Palavras-chave: Babesia microti; Liver; Histopathology.
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/334
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Studies of Liver of Mice in the Course of an Experimental Infection with Babesia microti. I : Histopathological Changes and Changes in the Glycogen Content OAK
Kuzna-Grygiel, Wanda; Kolodziejczyk, Lidia.
Palavras-chave: Babesia microti; Liver; Histopathology; Glycogen.
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/333
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The Number of MHC class Ⅱ Positive Splenic Antigen Presenting Cell in Babesia microti and Babesia rodhaini infected mice OAK
Hashiguchi-Kato, Rie; Shikano, Sojin; Shimada, Terumasa; Ono, Kenichiro.
Palavras-chave: Babesia microti; Babesia rodhaini; MHC class II; Th1; Th2.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/315
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The vector potential of haemaphysalis longicornis ticks for babesia microti parasites under experimental condition OAK
Kusakisako, K.; Maeda, H.; Galay, R.L.; Matsuo, T.; Tsujio, M.; Umemiya-Shirafuji, R.; Mochizuki, M.; Fujisaki, K.; Tanaka, T..
Palavras-chave: Babesia microti; Haemaphysalis longicornis; Transmission test; Vector.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4245
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国際獣医寄生虫学会(The 21st International Conference of the World Association for the Advancement of Veterinary Parasitology)での発表 OAK
井関, 博.
Palavras-chave: 人獣共通感染症; Loop-Mediated Isothermal Amplifi cation (LAMP); 診断; Babesia microti.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1822
Registros recuperados: 18
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