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A multivariate approach to differentiate yerba mate (Ilex paraguariensis) commercialized in the southern Brazil on the basis of phenolics, methylxanthines and in vitro antioxidant activity Ciênc. Tecnol. Aliment.
ZIELINSKI,Acácio Antonio Ferreira; ALBERTI,Aline; BONA,Evandro; BORTOLINI,Débora Gonçalves; BENVENUTTI,Laís; BACH,Fabiane; Demiate,Ivo Mottin; NOGUEIRA,Alessandro.
Abstract The consumption of yerba mate beverages is related to several health benefits. These desirable properties are mainly due to the bioactive compounds. However, the levels of those compounds are directly affected by factors such as geographical origin. This study aimed to use chemometrics to evaluate the antioxidant compounds of yerba mate (consumed as chimarrão) marketed in southern Brazil. Neochlorogenic acid and caffeine were the main bioactive compounds found in this type of yerba mate beverage (chimarrão). The dataset was analyzed by chemometrics, and principal component analysis using the first three principal components explained 61.30% of the total variance. Hierarchical cluster analysis suggested three clusters, with cluster 1 containing the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioactive compounds; Geographical origin; Chimarrão; HPLC; Pattern recognition.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020005006204
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Actiflagelin, a new sperm activator isolated from Walterinnesia aegyptia venom using phenotypic screening J. Venom. Anim. Toxins incl. Trop. Dis.
El-Aziz,Tarek Mohamed Abd; Khoury,Sawsan Al; Jaquillard,Lucie; Triquigneaux,Mathilde; Martinez,Guillaume; Bourgoin-Voillard,Sandrine; Sève,Michel; Arnoult,Christophe; Beroud,Rémy; Waard,Michel De.
Abstract Background Sperm contains a wealth of cell surface receptors and ion channels that are required for most of its basic functions such as motility and acrosome reaction. Conversely, animal venoms are enriched in bioactive compounds that primarily target those ion channels and cell surface receptors. We hypothesized, therefore, that animal venoms should be rich enough in sperm-modulating compounds for a drug discovery program. Our objective was to demonstrate this fact by using a sperm-based phenotypic screening to identify positive modulators from the venom of Walterinnesia aegyptia. Methods Herein, as proof of concept that venoms contain interesting compounds for sperm physiology, we fractionated Walterinnesia aegyptia snake venom by RP-HPLC and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Snake venom; Walterinnesia aegyptia; Bioactive compounds; Fertility; Sperm motility; Venomics; Tandem mass spectrometry; De novo sequencing; Edman degradation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100301
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Agronomic performance of 'Valência' orange combined with 13 rootstocks in the state of Rio Grande do Sul, Brazil. Repositório Alice
KIRINUS, M. B. M.; OLIVEIRA, R. P. de; SILVA, P. S. da; BARRETO, C. F.; MALGARIM, M. B.; SOARES FILHO, W. dos S..
bitstream/item/197746/1/Roberto-Pedroso-1678-3921-pab-54-e00461.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Citrus Sinensis; Bioactive compounds; Fruit quality.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109329
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Agronomic performance of 'Valência' orange combined with 13 rootstocks in the state of Rio Grande do Sul, Brazil PAB
Kirinus,Marines Batalha Moreno; Oliveira,Roberto Pedroso de; Silva,Pricila Santos da; Barreto,Caroline Farias; Malgarim,Marcelo Barbosa; Soares Filho,Walter dos Santos.
Abstract: The objective of this work was to evaluate the agronomic performance of 'Valência' sweet orange (Citrus sinensis) tree, in combination with 13 rootstocks cultivated in soil and climate conditions of the Brazilian state of Rio Grande do Sul. The following agronomic variables were evaluated: scion height and volume, plant production, production efficiency, productivity, fruit diameter, fruit mass, peel mass, juice yield, soluble solids content, titratable acidity, soluble solids:acidity ratio, ascorbic acid, peel color, antioxidant capacity, and phenolic compounds. A randomized complete block experimental design was used, with three replicates and nine plants per plot, in a two-factor arrangement (13 rootstocks and the 2015 and 2016 harvests). The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Bioactive compounds; Fruit quality.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102700
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An in vitro analysis of the total phenolic content, antioxidant power, physical, physicochemical, and chemical composition of Terminalia Catappa Linn fruits Ciênc. Tecnol. Aliment.
Marques,Marcelo Rodrigues; Paz,Diego Damasceno; Batista,Lívia Patrícia Rodrigues; Barbosa,Celma de Oliveira; Araújo,Marcos Antônio Mota; Moreira-Araújo,Regilda Saraiva dos Reis.
This study assessed the antioxidant, total phenolic, and physicochemical properties of in vitro Terminalia Catappa Linn (locally called castanhola) using the DPPH assay. The castanhola fruits had an average weight of 19.60 ± 0.00 g, combining shell, pulp, and seed weight, and a soluble solids content of 8 °Brix. The chemical composition was determined with predominance of carbohydrates (76,88 ± 0,58%).The titration method was used to determine Vitamin C content using 2,6-dichlorophenolindophenol (DCFI), known as reactive Tillmans resulting in no significant levels. Aqueous extracts of castanhola pulp showed a higher concentration of phenolics, 244.33 ± 18.86 GAE.g-1 of fruit, and alcoholic extracts, 142.84 ± 2.09 GAE.g-1 of fruit. EC50 values of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tropical fruit; Functional properties; Bioactive compounds.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100031
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Análise térmica de amidos de cará-moela incorporados com extratos aquosos de erva-mate (Ilex paraguariensis). Repositório Alice
WOLFF, S. M.; SILVEIRA, A. C. da; LAZZAROTTO, M..
O amido de Dioscorea bulbifera Linn, espécie conhecida também como cará moela, é um carboidrato pouco estudado. No entanto, a incorporação de extratos de erva-mate (Ilex paraguariensis) podem adicionar propriedades que agreguem valor ao produto. Este trabalho buscou adicionar extratos aquosos de erva-mate ao amido de D. bulbifera e avaliar os efeitos nas propriedades térmicas e reológicas. Assim, o amido nativo de D. bulbifera foi obtido e tratados com extrato aquosos de erva-mate em concentrações de 1,5%, 3% e 6%. Nas curvas TG, em atmosfera de ar sintético, dois eventos foram observados, no entanto a estabilidade e as propriedades térmicas do amido não foram alteradas significativamente. As temperaturas iniciais de gelatinização dos amidos incorporados...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Erva mate; Composto bioativo; TG-DSC; RVA; Ilex Paraguariensis; Composto Fenólico; Antioxidante; Phenolic compounds; Bioactive compounds; Antioxidant activity.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115122
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Antagonistic properties of some microorganisms isolated from Brazilian tropical savannah plants against Staphylococcus coagulase-positive strain J. Venom. Anim. Toxins incl. Trop. Dis.
Ratti,R. P.; Serrano,N. F. G.; Hokka,C. O.; Sousa,C. P..
Endophytic microorganisms are relatively unstudied as potential sources of novel natural products for medical and commercial exploitation. The aim of this work was to investigate some Brazilian tropical savannah trees Cassia leptophylla and Prunus spp. in order to isolate the endophytic microorganisms associated with these plants. The samples were disinfected to eliminate the epiphytic population. Colonies were diluted and displayed as drops in media and growing colonies were inactivated. Staphylococcus coagulase-positive strain was used as indicator microorganism and subjected to the antibioses test. Data showed that the microorganisms isolated from Cassia leptophylla had no inhibition against Staphylococcus. On the other hand, microorganisms isolated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic microorganisms; Plants; Antibacterial activity; Bioactive compounds.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200007
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Anthelmintic activity of nanoencapsulated carvacryl acetate against gastrointestinal nematodes of sheep and its toxicity in rodents. Repositório Alice
ANDRÉ, W. P. P.; PAIVA JUNIOR, J. R. de; CAVALCANTE, G. S.; RIBEIRO, W. L. C.; ARAÚJO FILHO, J. V. de; SANTOS, J. M. L. dos; ALVES, A. P. N. N.; MONTEIRO, J. P.; MORAIS, S. M. de; SILVA, I. N. G. da; OLIVEIRA, L. M. B. de; ABREU, F. O. M. da S.; BEVILAQUA, C. M. L..
Abstract: The objective of this study was to evaluate the efficacy of carvacryl acetate (CVA) and nanoencapsulated CVA (nCVA) on gastrointestinal nematodes of sheep. The CVA was nanoencapsulated with chitosan/gum arabic and the efficacy of nanoencapsulation (EE), yield, zeta potential, nanoparticle morphology and release kinetics at pH 3 and 8 were analyzed. Acute and subchronic toxicity were evaluated in rodents and reduction of egg counts in the faeces (FECRT) of sheep. The sheep were divided into four groups (n = 10): G1, 250 mg/kg CVA; G2, 250 mg/kg nCVA; G3, polymer matrix and G4: 2.5 mg/kg monepantel. EE and nCVA yield were 65% and 57%, respectively. The morphology of the nanoparticles was spherical, size (810.6±286.7 nm), zeta potential in pH 3.2...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Quitosana; Biocompostos; Acetilação; Nematoide gastrintestinal; Acetato de carvacrila; Segurança toxicológica; Biocompounds; Toxicological safety; Gastrointestinal parasitic nematodes; Ovino; Haemonchus Contortus; Helminto Gastrintestinal; Goma Arábica; Anti-Helmíntico; Parasitose; Chitosan; Gum arabic; Acetylation; Nematode control; Essential oils; Bioactive compounds.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125192
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Anthocyanin accumulation and color development of 'Benitaka' table grape subjected to exogenous abscisic acid application at different timings of ripening. Repositório Alice
SHAHAB, M.; ROBERTO, S. R.; AHMED, S.; COLOMBO, R. C.; SILVESTRE, J. P.; KOYAMA, R.; SOUZA, R. T. de.
In colored table grapes, the anthocyanin contents are inhibited by the high temperature during ripening and berries suffer a lack of skin color, thus affecting their market value. In order to overcome this issue, a research study was planned to evaluate the influence of (S)-cis-abscisic acid (S-ABA) on rates of anthocyanin accumulation in table grapes when applied at different timings of ripening, and to quantify the gradual increase of berry color. The study was conducted in a commercial vineyard of ?Benitaka? table grapes (Vitis vinifera L.), grown under double annual cropping system in a subtropical area. The trials were carried out during two consecutive seasons (i.e., summer season of 2015 and off-season of 2016). The treatments used for the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Vitis vinifera L; Production system; S-ABA; Rate of anthocyanin accumulation; CIRG; Bioactive compounds.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118223
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Antibacterial activity of Falkenbergia hillebrandii (Born) from the Indian coast against human pathogens Phyton
Manilal,A; Sujith,S; Selvin,J; Shakir,C; Seghal Kiran,G.
The antibacterial property of the red algae, Falkenbergia hillebrandii (Born) collected from the southwest coast of India (Indian Ocean) was evaluated against three multidrug resistant human pathogens. Four different solvents: ethyl acetate, dichloromethane, methanol and phosphate buffer saline (PBS) were used with this purpose. Dried samples extracted with methanol showed broadest and highest antimicrobial activity when compared to other solvents. However, PBS extract showed no antibacterial activity. The highly active compounds red alga, F. hillebrandii were fractionated and purified using different chromatographic systems, including reverse phase HPLC and GCMS. The analysis revealed that the most abundant metabolite was the oleic acid (51.33%) followed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; F. hillebrandii; Multidrug resistant human pathogens; Methanolic extract; Bioactive compounds.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000200013
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Antifungal activity of Pseudomonas frederiksbergensis CMAA 1323 isolated from the Antarctic hair grass Deschampsia antarctica. Repositório Alice
MELO, I. S. de; SOUZA, W. R.; SILVA, L. J.; SANTOS, S. N.; ASSALIN, M. R.; ZUCCHI, T. D.; QUEIROZ, S. C. do N. de.
Abstract: Aims: Epiphytic bacteria, isolated from Deschampsia antarctica, were screened for their potential to inhibit the plant pathogen Botrytis cinerea, the causal agent of gray mold disease of strawberry pseudofruits. This phytopathogenic fungus is more active and the disease is more serious in temperate climate where the temperatures are lower.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Psychrophilic bacteria; Pseudomonas frederiksbergensis; Bioactive compounds; Diketopiperazines; Deschampsia antarctica; Compostos bioativos; Botrytis cinerea; Bactéria; Controle biológico; Diketopiperazines; Deschampsia antarctica; Pseudomonas frederiksbergensis; Psychrophilic bacteria; Antifungal agents.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1054794
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Antimicrobial activity and bioactive compounds of portuguese wild edible mushrooms methanolic extracts IPB - Escola Superior Agrária
Barros, Lillian; Calhelha, Ricardo C.; Vaz, Josiana A.; Ferreira, Isabel C.F.R.; Baptista, Paula; Estevinho, Leticia M..
The antimicrobial properties of phenolic extracts of Portuguese wild edible mushroom species (Lactarius deliciosus, Sarcodon imbricatus and Tricholoma portentosum) against pathogens were investigated. The minimal inhibitory concentrations (MICs) were evaluated for the entire mushroom, the cap and the stipe, separately; the portion of the mushroom used proved to be influenced in the results obtained, which are directly correlated with the content of total phenols and flavonoids in the extracts. The growth of Grampositive bacteria (Bacillus cereus, B. subtilis,) was well inhibited by these mushrooms, while Escherichia coli (Gramnegative bacteria) was resistant. The study on the antifungal effect of these mushrooms revealed that Candida albicans and...
Tipo: Article Palavras-chave: Wild edible mushrooms; Bioactive compounds; Antimicrobial activity.
Ano: 2007 URL: http://hdl.handle.net/10198/829
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Antimicrobial activity and chemical characterization of the bark decoction of cumaru stem Ciência Rural
Ferreira,Maria Jaiana Gomes; Nogueira,Patrícia Coelho do Nascimento; Dias,Flayanna Gouveia Braga; Silva,Larissa Morais Ribeiro da; Silveira,Edilberto Rocha; Figueiredo,Evânia Altina Teixeira de.
ABSTRACT: The plant, Amburana cearensis A. C. Smith (Fabaceae), commonly called cumaru, is widespread in the Caatinga cearense, a less known ecosystem in Brazil. A. cearensis is rich in several compounds like protocatechuic acid, tannins, coumarin, flavonoids and phenolic heterosides, such as amburosides A and B, that have been isolated. The aim of this study was to determine the antimicrobial potential and draw the chemical profile of the distinct characteristics of A. cearensis stem bark decoction, for its possible potential as a food conservation agent. The chemical compounds were characterized by one- and two-dimensional 1H and 13C NMR analyses and Liquid Chromatography-Mass Spectrometry (LCMS). The compounds of coumarin, amburosides A and B, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioactive compounds; Microorganisms; Natural antimicrobials.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000300452
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Antimicrobial, Antioxidant and Cytotoxic Activity of MarineStreptomyces parvulus VITJS11 Crude Extract BABT
Naine,S. Jemimah; Devi,C. Subathra; Mohanasrinivasan,V.; B,Vaishnavi.
The main aim of the study was to evaluate the bioactive properties of ethyl acetate crude extract of Streptomyces parvulus VITJS11 with a view to assess their therapeutic potential. The biological activity of ethyl acetate extract was tested against fungal and bacterial pathogens. The free radical scavenging potential of the crude extract was determined by DPPH assay. The chemo preventive properties of S. parvulus VITJS11 ethyl acetate extract was examined by MTT assay on HepG2 cells. The morphological, physiological and the biochemical properties of the strain S. parvulus VITJS11 was confirmed by conventional methods. Genotypic characterization was done using 16S r-DNA partial gene amplification and sequencing. The authenticity of the crude chemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Marine actinomycetes; Streptomyces parvulusVITJS11; Antimicrobial activity; Antioxidants; Cytotoxicity; Bioactive compounds.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200198
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Antioxidant potential and cytotoxic activity of two red seaweed species, Amansia multifida and Meristiella echinocarpa, from the coast of Northeastern Brazil Anais da ABC (AABC)
ALENCAR,DANIEL B. DE; SILVA,SUZETE R. DA; PIRES-CAVALCANTE,KELMA M.S.; LIMA,REBECA L. DE; PEREIRA JÚNIOR,FRANCISCO N.; SOUSA,MÁRCIA B. DE; VIANA,FRANCISCO A.; NAGANO,CELSO S.; NASCIMENTO,KYRIA S. DO; CAVADA,BENILDO S.; SAMPAIO,ALEXANDRE H.; SAKER-SAMPAIO,SILVANA.
Natural antioxidants found in marine macroalgae are bioactive compounds known to play an important role in the prevention of diseases associated with aging cells protecting them against the oxidative damage. The purpose of this study was to evaluate the antioxidant and cytotoxic activity of ethanolic extracts of two species of red seaweeds, Amansia multifida and Meristiella echinocarpa. In vitro antioxidant activity was determined by DPPH radical scavenging assay, ferric-reducing antioxidant power (FRAP) assay, ferrous ion chelating (FIC) assay, β-carotene bleaching (BCB) assay and total phenolic content (TPC) quantification. Cytotoxicity was evaluated with the brine shrimp Artemia sp. lethality test. The TPC values observed in the present study indicated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Bioactive compounds; Cytotoxic; Phenolic compounds; Rhodophyta.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000100251
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Apple extract: cytotoxicity and dna protection. Repositório Alice
VIZZOTTO, M.; FERRI, N. M. L.; BIALVES, T. S.; ARAUJO, V. F.; PEREIRA, E. dos S.; KROLOW, A. C. R..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Bioactive compounds; Crystal violet; Comet assay.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/983037
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Application of enzymatic preparations to produce araçá pulp and juice Ciênc. Tecnol. Aliment.
Sandri,Ivana Greice; Piemolini-Barreto,Luciani Tastch; Fontana,Roselei Claudete; Silveira,Mauricio Moura da.
In the present study, the effect of pectinases on the production of pulp and juice of araçá and the presence of bioactive compounds were evaluated. An enzyme extract (EE) produced by Aspergillus niger LB-02-SF in solid state fermentation and the commercial enzyme Ultrazym®AFP-L were used in this study. After enzyme treatment with the EE preparation, the extraction yield increased by 23.1% and viscosity decreased by 42.8%, during pulp maceration. During juice processing, there was an increase of 70.6% in clarification and a decrease of 72.87% in turbidity. Higher values of these parameters, 47.7, 69.0, 80.7, and 79.7%, respectively, were obtained using the Ultrazym®AFP-L, which also led to a significant increase in the polyphenol content, both in the pulp...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Yield; Clarification; Bioactive compounds.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000400002
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Avocado: characteristics, health benefits and uses Ciência Rural
Duarte,Patrícia Fonseca; Chaves,Marcia Alves; Borges,Caroline Dellinghausen; Mendonça,Carla Rosane Barboza.
ABSTRACT: This study aimed to present a literature review about the characteristics, applications, and potential of avocado (Persea americana). Avocado is considered one of the main tropical fruits, as it contains fat-soluble vitamins which are less common in other fruits, besides high levels of protein, potassium and unsaturated fatty acids. Avocado pulp contains variable oil content, and is widely used in the pharmaceutical and cosmetics industry, and in the production of commercial oils similar to olive oil. This fruit has been recognized for its health benefits, especially due to the compounds present in the lipidic fraction, such as omega fatty acids, phytosterols, tocopherols and squalene. Studies have shown the benefits of avocado associated to a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avocado tree; Avocado oil; Unsaturated fatty acid; Bioactive compounds.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400747
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Bioactive compounds and antioxidant activity analysis during orange vinegar production Ciênc. Tecnol. Aliment.
DAVIES,Cristina Verónica; GERARD,Liliana Mabel; FERREYRA,María Mercedes; SCHVAB,María del Carmen; SOLDA,Carina Alejandra.
Abstract Citrus fruits are significant sources of bioactive compounds, such as ascorbic acid, polyphenols and carotenoids, due to their antioxidant properties important for human nutrition. In addition, since oranges possess high sugar content (8-15%), making vinegars from alcoholic orange substrates, with functional characteristics is a possible development of novel products. The aim of this research work was to analyze changes in ascorbic acid, total phenolics, total carotenoids and antioxidant activity during orange vinegar processing. In order to analyze the influence of acetification and aging in these characteristics, samples were taken in three stages: orange alcoholic substrate for acetification (SNA), young orange vinegar or recently obtained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Orange vinegar; Antioxidant activity; Bioactive compounds.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000300449
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Bioactive compounds and chemical composition of Brazilian Cerrado fruits’ wastes: pequi almonds, murici, and sweet passionfruit seeds Ciênc. Tecnol. Aliment.
ARAÚJO,Ana Cristina Moreira Andrade; MENEZES,Evandro Galvão Tavares; TERRA,André William Costa; DIAS,Bruna Oliveira; OLIVEIRA,Érica Resende de; QUEIROZ,Fabiana.
Abstract Pequi, murici and sweet passionfruit are typical fruits from Brazilian Cerrado, which stand out for their sensory attributes such as color, flavor and aroma, in addition to their high nutritional value. Their seeds are by-products from the industrial processing of juices, pulps, jellies, and others, and have great exploitation potential due to their high oil content and the presence of bioactive compounds. The present work aimed to evaluate the chemical composition of pequi almonds, and of murici and sweet passionfruit seeds, and also to quantify the total phenolic compounds, carotenoids, anthocyanins and antioxidant activity by the scavenging activity of •DPPH method. Concerning the chemical composition, pequi almonds presented the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioactive compounds; Antioxidants; Polyphenols; Anthocyanins; Nutritional composition.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000500203
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